1.1.1.11: D-arabinitol 4-dehydrogenase

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For detailed information about D-arabinitol 4-dehydrogenase, go to the full flat file.

Reaction

Synonyms

aArDH, arabitol dehydrogenase, ArDH, D-arabitol dehydrogenase, D-xylulose-forming D-arabitol dehydrogenase, dehydrogenase, D-arabinitol, NAD-dependent D-arabitol dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.11 D-arabinitol 4-dehydrogenase

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Results	in table
1	Activating Compound
105	AA Sequence
5	Application
1	CAS Registry Number
9	Cloned(Commentary)
24	Cofactor
2	Expression
2	General Information
2	General Stability
6	Inhibitors
6	kcat/KM [mM/s]
29	KM Value [mM]
5	Metals/Ions
15	Molecular Weight [Da]
17	Natural Substrates/ Products (Substrates)
43	Organism
6	Pathway
9	pH Optimum
5	pH Range
2	pH Stability
2	pI Value
1	Protein Variants
7	Purification (Commentary)
1	Reaction
3	Reaction Type
18	Reference
33	Specific Activity [micromol/min/mg]
5	Storage Stability
49	Substrates and Products (Substrate)
10	Subunits
12	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
12	Turnover Number [1/s]

Expression

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Expression on EC 1.1.1.11 - D-arabinitol 4-dehydrogenase

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

ArDH enzyme expression in tobacco chloroplasts confers tolerance to NaCl (up to 400 mM). Transgenic plants compared to wild-type survived for only 4-5 weeks on 400 mM NaCl whereas plants remain green and grow normal on concentrations up to 350 mM NaCl. A-week-old seedlings are also challenged with PEG up to 6% in the liquid medium, considering that membranes and proteins are protected under stress conditions due to accumulation of arabitol in chloroplasts. Seedlings are tolerant to 6% PEG, suggesting that ARDH enzyme maintains integrity of membranes in chloroplasts under drought conditions via metabolic engineering

Saccharomyces cerevisiae

-

740490

induced by D-arabinitol

Gluconobacter oxydans

Q308C1

701082