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1.1.1.141: 15-hydroxyprostaglandin dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about 15-hydroxyprostaglandin dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.141

Reaction

(5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprost-5,13-dienoate
+
NAD+
=
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate
+
NADH
+
H+

Synonyms

11alpha,15-dihydroxy-9-oxoprost-13-enoate:NAD+ 15-oxidoreductase, 15-hydroxy prostaglandin dehydrogenase, 15-hydroxy-prostaglandin-dehydrogenase, 15-hydroxyprostaglandin dehydrogenase, 15-hydroxyprostaglandin-dehydrogenase, 15-hydroxyprostanoic dehydrogenase, 15-OH-PGDH, 15-PGDH, 15-prostaglandin dehydrogenase, dehydrogenase, 15-hydroxyprostaglandin, HPGD, NAD+ dependent 15-hydroxyprostaglandin dehydrogenase, NAD+ dependent PGDH, NAD+-15-hydroxy prostanoate oxidoreductase, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (type I), NAD+-dependent 15-PGDH, NAD+-linked 15-hydroxyprostaglandin dehydrogenase, NAD-dependent 15-hydroxyprostaglandin dehydrogenase, NAD-specific 15-hydroxyprostaglandin dehydrogenase, nicotinamide adenine dinucleotide-dependent 15-hydroxyprostaglandin dehydrogenase, PGDH, prostaglandin dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.141 15-hydroxyprostaglandin dehydrogenase (NAD+)

Purification

Purification on EC 1.1.1.141 - 15-hydroxyprostaglandin dehydrogenase (NAD+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CM-Sepharose, DEAE-Sepharose, Blue Sepharose, Mono-Q
-
glutathione Sepharose column chromatography
native enzyme 2600fold from lung cytosol of pregnant rabbits, by cation and anion exchange chromatography, gel filtration, hydrophobic interaction and hydroxylapatite chromatography to homogeneity
-
native enzyme from lung by ammonium sulfate fractionation and acetone precipitation
-
native enzyme partially from heart
-
native enzyme partially from kidney
-
native soluble enzyme partially from heart ventricular tissue by ultracentrifugation, ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
-
native soluble enzyme partially from lung
-
native soluble enzyme partially from lung by ultracentrifugation, ammonium sulfate fractionation and anion exchange chromatography
-
nickel-Sepharose column chromatography
-
partial
recombinant 15-PGDH
-
recombinant His-tagged lung enzyme from Escherichia coli by nickel affinity chromatography