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1.1.1.176: 12alpha-hydroxysteroid dehydrogenase

This is an abbreviated version!
For detailed information about 12alpha-hydroxysteroid dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.176

Reaction

cholate
+
NADP+
=
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate
 +
NADPH
 +
H+

Synonyms

12alpha-HSDH, 12alpha-hydroxy steroid dehydrogenase, CDD59475, dehydrogenase, 12alpha-hydroxy steroid, Elen_2515, NAD+-dependent 12alpha-HSDH, NAD-dependent 12alpha-hydroxysteroid dehydrogenase, NADP+-dependent 12alpha-HSDH, NADP-12alpha-hydroxysteroid dehydrogenase, NADP-dependent 12alpha-hydroxysteroid dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.176 12alpha-hydroxysteroid dehydrogenase

Substrates Products

Substrates Products on EC 1.1.1.176 - 12alpha-hydroxysteroid dehydrogenase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12-ketochenodeoxycholic acid + NADH
cholic acid + NAD+
show the reaction diagram
-
-
-
r
12-oxo-cholic acid + NAD(P)H + H+
cholic acid + NAD(P)+
show the reaction diagram
4.1% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
12-oxo-deoxycholic acid + NAD(P)H + H+
deoxycholic acid + NAD(P)+
show the reaction diagram
-
-
-
r
12-oxochenodeoxycholic acid + NADP+
(3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxycholan-24-oic acid + NADPH + H+
show the reaction diagram
-
-
-
-
?
12-oxochenodeoxycholic acid + NADPH + H+
7-oxodeoxycholic acid + NADP+
show the reaction diagram
12-oxolithocholic acid + NADH + H+
deoxycholic acid + NAD+
show the reaction diagram
12-oxolithocholic acid + NADPH + H+
deoxycholic acid + NADP+
show the reaction diagram
3-oxocholic acid + NADPH + H+
cholic acid + NADP+
show the reaction diagram
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl methyl ester + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl methyl ester + NADPH + H+
show the reaction diagram
-
-
-
-
r
5beta-cholanic acid-3,12-dione + NADPH + H+
3-oxodeoxycholic acid + NADP+
show the reaction diagram
5beta-cholanic acid-3,7,12-trione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
show the reaction diagram
5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
show the reaction diagram
7-oxocholic acid + NADPH + H+
deoxycholic acid + NADP+
show the reaction diagram
7alpha,12alpha-dihydroxy-5beta-cholanoate + NAD+
7alpha-hydroxy-12-oxo-5beta-cholanoate + NADH
show the reaction diagram
-
-
-
-
?
allocholate + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH
show the reaction diagram
-
-
-
-
?
allodeoxycholate + NAD+
3alpha-hydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH
show the reaction diagram
-
-
-
-
?
cholate + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
show the reaction diagram
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
show the reaction diagram
cholic acid + NAD(P)+
12-oxocholic acid + NAD(P)H + H+
show the reaction diagram
55.7% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
show the reaction diagram
cholic acid + NAD+
3-oxocholic acid + NADH + H+
show the reaction diagram
cholic acid + NADP+
12-ketochenodeoxycholic acid + NADPH + H+
show the reaction diagram
-
the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview
-
-
r
cholic acid + NADP+
3-oxocholic acid + NADPH + H+
show the reaction diagram
deoxycholate + NADP+
12-oxo-lithocholic acid + NADPH + H+
show the reaction diagram
deoxycholic acid + NAD(P)+
12-oxo-deoxycholic acid + NAD(P)H + H+
show the reaction diagram
51.9% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
deoxycholic acid + NAD(P)+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
show the reaction diagram
deoxycholic acid + NAD+
12-oxolithocholic acid + NADH + H+
show the reaction diagram
-
100% activity
-
-
r
deoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
deoxycholic acid + NADP+
12-oxolithocholic acid + NADH + H+
show the reaction diagram
-
3.14% activity compared to NAD+
-
-
r
deoxycholic acid + NADP+
7-oxocholic acid + NADPH + H+
show the reaction diagram
glycocholic acid + NAD(P)+
? + NAD(P)H + H+
show the reaction diagram
60.3% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
show the reaction diagram
glycodeoxycholic acid + NAD(P)+
? + NAD(P)H + H+
show the reaction diagram
55.9% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
glycodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
show the reaction diagram
glycodeoxycholic acid + NAD+
? + NADH + H+
show the reaction diagram
-
97.8% activity compared to deoxycholic acid
-
-
r
glycodeoxycholic acid + NADP+
? + NADPH + H+
show the reaction diagram
taurocholic acid + NAD(P)+
2-[(3alpha,7alpha-dihydroxy-12,24-dioxo-5beta-cholan-24-yl)amino]ethane-1-sulfonic acid + NAD(P)H + H+
show the reaction diagram
53.8% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
taurocholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
show the reaction diagram
taurocholic acid + NAD+
? + NADH + H+
show the reaction diagram
-
98.95% activity compared to deoxycholic acid
-
-
r
taurodeoxychloic acid + NAD(P)+
? + NAD(P)H + H+
show the reaction diagram
58.9% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
taurodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH
show the reaction diagram
taurodeoxycholic acid + NAD+
? + NADH + H+
show the reaction diagram
-
90.84% activity compared to deoxycholic acid
-
-
r
taurodeoxycholic acid + NADP+
? + NADPH + H+
show the reaction diagram
additional information
?
-