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1.1.1.219: dihydroflavonol 4-reductase

This is an abbreviated version!
For detailed information about dihydroflavonol 4-reductase, go to the full flat file.

Word Map on EC 1.1.1.219

Reaction

a (2R,3S,4S)-leucoanthocyanidin
+
NADP+
=
a (2R,3R)-dihydroflavonol
+
NADPH
+
H+

Synonyms

Bo9g058630, BrDFR1, BrDFR10, BrDFR11, BrDFR12, BrDFR2, BrDFR3, BrDFR4, BrDFR5, BrDFR6, BrDFR7, BrDFR8, BrDFR9, CsDFR, Cyp82C1, cytochrome P450 CYP82C1, DcDFR, DFL1, DFR, DFR1, DFR1a, DFR2, DFR3, DFR4a, DFR4b, DFR5, DgDFR, DHM reductase, DHQ reductase, dihydroflavanol 4-reductase, dihydroflavanol-4-reductase-like protein, dihydroflavonol 4-reductase, dihydroflavonol reductase, dihydroflavonol-4-reductase, dihydrokaempferol 4-reductase, dihydromyricetin reductase, dihydroquercetin reductase, DnDFR, flavan-3-ol NADPH-dihydromycertin reductase, flavonoid 3'-hydroxylase, GbDFR1, GbDFR2, GbDFR3, IbDFR, McDFR, NADPH-dihydromyricetin reductase, NtDFR1, PAS_chr3_0449, PgDFR, PhDFR, reductase, dihydromyricetin, reductase, dihydroquercetin, RrDFR1, TaDFR, TaDFR-A, TaDFR-I, TRANSPARENT TESTA 3 protein

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.219 dihydroflavonol 4-reductase

Crystallization

Crystallization on EC 1.1.1.219 - dihydroflavonol 4-reductase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
molecular docking of substrates. A phenol ring of dihydrokaempferol, a catechol ring of dihydroquercetin, and a pyrogallol ring of dihydromyricetin respectively have two, three, and four hydrogen bonds formed with side chains of residue Asn132
molecular docking of substrates. The number of hydrogen bonds is one, two, and two for the corresponding phenol, catechol, and pyrogallol rings, respectively
DFR-NADP+-flavonol (myricetin and quercetin) complexes, at room temperature, by hanging-drop vapour diffusion method. Complex DFR-NADP+-myricetin belongs to space group P21 (unit-cell parameters a = 47.23, b = 177.96, c = 92.60 A, beta = 104.8°). Complex DFR-NADP+-quercetin belongs to space group P6122 (unit-cell parameters a = b = 174.94, c = 290.18 A)
-
purified recombinant enzyme in complex with NADPH and dihydroquercetin, X-ray diffraction structure determination and analysis at 1.8 A resolution
-