1.1.1.227: (-)-borneol dehydrogenase

This is an abbreviated version!
For detailed information about (-)-borneol dehydrogenase, go to the full flat file.

Reaction

Synonyms

BDH, BDH1, borneol dehydrogenase, EC 1.1.1.182, LiBDH, LiSDR-2, TCK1_3449, THL1_4180

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.227 (-)-borneol dehydrogenase

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Results	in table
1	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
4	Cofactor
5	General Information
1	General Stability
2	Inhibitors
1	kcat/KM [mM/s]
4	Ki Value [mM]
11	KM Value [mM]
2	Localization
2	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
12	Organism
3	Pathway
5	pH Optimum
2	pI Value
4	Protein Variants
5	Purification (Commentary)
1	Reaction
3	Reaction Type
8	Reference
1	Renatured (Commentary)
5	Source Tissue
1	Storage Stability
16	Substrates and Products (Substrate)
7	Subunits
14	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
9	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.227 - (-)-borneol dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

native enzyme from strain TCU-HL1 by hydrophobic interaction and anion exchange chromatography, recombinant refolded enzyme from Escherichia coli

Pseudomonas sp.

-

739915

partial

Tanacetum vulgare

-

286162

partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns

Pseudomonas monteilii

-

761009

production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium

Pseudomonas sp. TCU-HL1

A0A1B3EB36

762321

recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLysSby nickel affinity chromatography

Lavandula x intermedia

-

724125