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1.1.1.236: tropinone reductase II

This is an abbreviated version!
For detailed information about tropinone reductase II, go to the full flat file.

Word Map on EC 1.1.1.236

Reaction

pseudotropine
+
NADP+
=
tropinone
+
NADPH
+
H+

Synonyms

DnTR2, More, pseudotropine forming tropinone reductase, pseudotropine reductase II, pseudotropine-forming tropinone reductase, pseudotropinone forming tropinone reductase, TR-II, TR2, TRII, tropinone (psi-tropine-forming) reductase, tropinone reductase, tropinone reductase I, tropinone reductase II, tropinone reductase-II

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.236 tropinone reductase II

Cloned

Cloned on EC 1.1.1.236 - tropinone reductase II

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA coding for TRI and TRII isolated and expressed in Escherichia coli NM522, P29X-encoding gene detected
-
cDNA encoding tropinone reductase II
-
cDNA homologous to pseudotropine-forming tropinone reductase isolated, recombinant protein functionally expressed in Escherichia coli BL21(DE3), transformed with pET21d vectors
-
expressed in Escherichia coli
full-length sequences determined of 5 clones with high homology to TRs
-
gene tr, DNA and amino acid sequence determination and analysis, sequence comparison
gene TR2, enzyme overexpression via leaves transformation with Agrobacterium tumefaciens AGL0, quantitative PCR enzyme expression analysis
-
gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview
-
gene TRII, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), realtime PCR enzyme expression analysis
overexpression in Atropa belladonna. Transformation with cDNA of tropinone reductases successfully alters the ratio of tropine-derived alkaloids versus pseudotropine-derived alkaloids
P29X-encoding gene detected
-
quantitative real-time PCR enzyme expression analysis
TRII cDNA subcloned from pTR2EN induced in Escherichia coli BL21(DE3)
-
TRII cDNA, in Escherichia coli expression vector pET21d
TRII, DNA and amino acid sequence determiation and analysis, phylogenetic analysis and tree, quantitative real-time PCR expression level analysis, recombinant epression in Escherichia coli strain Rosetta (DE3)