1.1.1.263: 1,5-anhydro-D-fructose reductase

This is an abbreviated version!
For detailed information about 1,5-anhydro-D-fructose reductase, go to the full flat file.

Reaction

Synonyms

1,4-AF-reductase, AF reductase, AFR, AKR1E1, More, NADP(H)-dependent 1,5-anhydro-D-fructose reductase, NADP-1,5-anhydro-D-fructose reductase, NADPH dependent 1,5-anhydro-D-fructose reductase, NADPH-dependent anhydrofructose reductase, NADPH-dependent monomeric reductase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.263 1,5-anhydro-D-fructose reductase

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Results	in table
1	Activating Compound
14	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
8	Cofactor
2	Crystallization (Commentary)
1	General Stability
13	Inhibitors
29	KM Value [mM]
1	Metals/Ions
6	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
12	Organism
6	PDB ID
4	pH Optimum
1	pH Stability
1	pI Value
14	Protein Variants
4	Purification (Commentary)
1	Reaction
7	Reaction Type
10	Reference
4	Source Tissue
15	Specific Activity [micromol/min/mg]
3	Storage Stability
33	Substrates and Products (Substrate)
4	Subunits
16	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
17	Turnover Number [1/s]

Inhibitors

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Inhibitors on EC 1.1.1.263 - 1,5-anhydro-D-fructose reductase

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1,5-anhydro-D-glucitol

Sus scrofa

-

2.3% inhibition at 100 mM

207965

2-mercaptoethanol

Sus scrofa

-

2.3% inhibition at 1 mM

207965

ascorbic acid

Sus scrofa

-

30% inhibition at 1 mM

207965

Barbital

Sus scrofa

-

15% inhibition at 1 mM

207965

D-glucose

Sus scrofa

-

2.6% inhibition at 100 mM

207965

D-glucose-1-phosphate

Sus scrofa

-

25.5% inhibition at 50 mM

207965

D-Glucose-6-phosphate

Sus scrofa

-

2% inhibition at 50 mM

207965

EDTA

Sus scrofa

-

59% inhibition at 10 mM

207965

iodoacetamide

Sus scrofa

-

41% inhibition at 1 mM

207965

iodoacetic acid

Sus scrofa

-

27% inhibition at 1 mM

207965

p-chloromercuribenzoic acid

Sus scrofa

-

79% inhibition at 0.1 mM, partially reversed by 0.1 mM 2-mercaptoethanol

207965

Phenobarbital

Sus scrofa

-

22% inhibition at 1 mM

207965

additional information

Ensifer adhaerens

-

activity of AFR was not influenced by 1 mM EDTA or by any of the given metal salts NaCl, KCl, MgCl2, CaCl2, MnCl2, FeCl3, and ZnCl2, 1 mM each

667264

-