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1.1.1.272: D-2-hydroxyacid dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about D-2-hydroxyacid dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.272

Reaction

an (R)-2-hydroxycarboxylate
+
NADP+
=
a 2-oxocarboxylate
 +
NADPH
 +
H+

Synonyms

(R)-2-hydroxyacid dehydrogenase, (R)-sulfolactate dehydrogenase, (R)-sulfolactate:NAD(P)+ oxidoreductase, 2-D-hydroxyacid dehydrogenase, 2-HDH, 2HADH, D-2-hydroxyacid dehydrogenase, D-isomer specific 2-hydroxyacid dehydrogenase, D2-HDH, DDH, DDH_HALMT, HDH, L-2-hydroxyacid dehydrogenase, L-sulfolactate dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.272 D-2-hydroxyacid dehydrogenase (NADP+)

Crystallization

Crystallization on EC 1.1.1.272 - D-2-hydroxyacid dehydrogenase (NADP+)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
free enzyme, to 3.0 A resolution, and the nonproductive ternary complex with alpha-ketohexanoic acid and NAD+ to 2.0 A resolution, by hanging-drop vapour-diffusion method
three crystal structures of DDH_HALMT are solved in complex with combinations of NAD+, NADP+, NADPH, 2-ketohexanoic acid, and 2-hydroxyhexanoic acid (PDB IDs are 5mha, 5mh5, 5mh6)
purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 30 mg/ml protein in 25 mM Tris/HCl, pH 8.0, 0.3 M NaCl, and 1 mM dithiothreitol, with 0.001 ml of reservoir solution containing 23% w/v PEG 3350, 0.2 M MgCl2, and 0.1 M HEPES, pH 7.0, and equilibration against 0.1 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 1.64 A resolution, molecular replacement and modeling
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