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1.1.1.274: 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming), go to the full flat file.

Word Map on EC 1.1.1.274

Reaction

2-dehydro-D-gluconate
+
NADP+
=
2,5-didehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-gluconate reductase, 2,5-DKG, 2,5-DKG reductase, 2,5-DKGR, 2,5DKGR, beta-keto ester reductase, CTATCC11996_22452, Dkr, YafB, YqhE, YqhE reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.274 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

Storage Stability

Storage Stability on EC 1.1.1.274 - 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, 20 mM Tris-HCl, pH 7.5, over 6 months, no loss of activity
-
4°C, pH 6.5-7.5, at least 2 months, no loss of activity
-
4°C, purified enzyme, 400 mM potassium phoshate buffer, pH 7.0, several weeks without appreciable loss of activity
-