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1.1.1.280: (S)-3-hydroxyacid-ester dehydrogenase

This is an abbreviated version!
For detailed information about (S)-3-hydroxyacid-ester dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.280

Reaction

ethyl (S)-3-hydroxyhexanoate
+
NADP+
=
ethyl 3-oxohexanoate
+
NADPH
+
H+

Synonyms

2-methyl-3-oxobutanoate reductase, 3-oxo ester (S)-reductase, EC 1.2.1.56, reductase, 3-oxo ester (S)-

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.280 (S)-3-hydroxyacid-ester dehydrogenase

Purification

Purification on EC 1.1.1.280 - (S)-3-hydroxyacid-ester dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography, and gel filtration
-
using streptomycin sulfate treatment, Sephadex G-25 filtration, DEAE-Sepharose CL-6B chromatography, Sephadex G-150 filtration, Sepharose 6B filtration and hydroxyapatite chromatography
-