1.1.1.286: isocitrate-homoisocitrate dehydrogenase
This is an abbreviated version!
For detailed information about isocitrate-homoisocitrate dehydrogenase, go to the full flat file.
Reaction
Synonyms
3-carboxy-2-hydroxyadipate dehydrogenase, HICDH, homoisocitrate dehydrogenase, isocitrate-homoisocitrate dehydrogenase, More, Saci_2375, TK0280
ECTree
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Engineering
Engineering on EC 1.1.1.286 - isocitrate-homoisocitrate dehydrogenase
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R87T
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uses substrate homoisocitrate, but not substrates isocitrate or 3-isopropylmalate
R87V
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uses substrate homoisocitrate, but not substrates isocitrate or 3-isopropylmalate
I82N
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
L81P
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
L83R
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
S80A
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
T71V
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
I82N
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site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
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L81P
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site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
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S80A
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site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
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T71V
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site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme
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R85V
additional information
complete loss of activity with citrate, retains activity with isocitrate
R85V
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
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complete loss of activity with citrate, retains activity with isocitrate
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deletion of A80, inability of mutant to use 3-isopropylmalate, isocitrate is 4fold preferred over homoisocitrate
additional information
generation of a gene-disruption TK0280 deletion mutan strain, functional complementation by expression of the gene icdh from Thermus thermophilus strain Hb27, but not by genes hicdh and leuB from this organism
additional information
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generation of a gene-disruption TK0280 deletion mutan strain, functional complementation by expression of the gene icdh from Thermus thermophilus strain Hb27, but not by genes hicdh and leuB from this organism
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