1.1.1.304: diacetyl reductase [(S)-acetoin forming]

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For detailed information about diacetyl reductase [(S)-acetoin forming], go to the full flat file.

Word Map on EC 1.1.1.304

1.1.1.304

lactis

alpha-dicarbonyls

2,3-pentanedione

pigeon

l-xylulose

xylitol

alpha-acetolactate

diacetylactis

lager

s-acetoin

synthesis

Reaction

Synonyms

acetoin(diacetyl) reductase, ADH, AdR, ADS1, ardII, BSDR, budC, DAR, diacetyl reductase, EC 1.1.1.5, FQU75_06005, Kp-BudC, Kpdar, NADH-dependent Kp-BudC, Ppdar, ReADR, S-stereospecific diacetyl reductase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.304 diacetyl reductase [(S)-acetoin forming]

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Results	in table
1	Activating Compound
3781	AA Sequence
4	Application
10	Cloned(Commentary)
22	Cofactor
7	General Information
1	General Stability
18	Inhibitors
8	kcat/KM [mM/s]
3	Ki Value [mM]
32	KM Value [mM]
4	Metals/Ions
14	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
23	Organism
5	Pathway
4	PDB ID
11	pH Optimum
5	pH Range
1	pH Stability
4	pI Value
6	Protein Variants
7	Purification (Commentary)
2	Reaction
21	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
1	Storage Stability
51	Substrates and Products (Substrate)
11	Subunits
47	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
7	Turnover Number [1/s]

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Cloned on EC 1.1.1.304 - diacetyl reductase [(S)-acetoin forming]

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expression in Escherichia coli

2 entries

gene adr, DNA and amino acid sequence determination and analysis, sequence comparison

Rhodococcus erythropolis

C0ZPN9

721396

gene adr, DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged enzyme in Escherichia coli

Rhodococcus erythropolis

C0ZPN9

721396

gene budC, functional recombinant expression of NADH-dependent Kp-BudC from plasmid pKM3 in Escherichia coli wild-type strain BW25113 and in mutant strain JCL166 (BW25113/F' [traD36, proABþ, lacIqZ DELTAM15 (Tet)] JCL16 DELTAadhE DELTAldhA DELTAfrdBC)

Klebsiella pneumoniae

Q48436

763422

gene dar, fucntional expression in Escherichia coli strain Rosetta (DE3), resulting in production of S-acetoin with higher than 99.9% optical purity from diacetyl

Paenibacillus polymyxa

KC505218

725854

recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha, coexpression with formate dehydrogenase (FDH) gene fdh from Saccharomyces cerevisiae

3 entries

Klebsiella pneumoniae

D7RP28

763688