Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.1.1.307: D-xylose reductase [NAD(P)H]

This is an abbreviated version!
For detailed information about D-xylose reductase [NAD(P)H], go to the full flat file.

Word Map on EC 1.1.1.307

Reaction

xylitol
+
NAD(P)+
=
D-xylose
+
NAD(P)H
+
H+

Synonyms

AKR2B5, ALR2, CTHT_0056950, CtXR, dsXR, dual specific xylose reductase, KmXYL1, NAD(P)H-dependent D-xylose reductase, NAD(P)H-dependent D-xylose reductase-like protein, NAD(P)H-dependent XR, NAD(P)H-dependent xylose reductase, NAD(P)H-linked xylose reductase, NADH/NADPH-xylose reductase, NADP-dependent xylose reductase, PsXR, PsXYL1, SaXYL1, SpXYL1.1, SsXR, Texr, XR, XYL1, xylose reductase, XylR, XyrA

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.307 D-xylose reductase [NAD(P)H]

Cloned

Cloned on EC 1.1.1.307 - D-xylose reductase [NAD(P)H]

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3), subloned into the pYES2 vector and transformed into Saccharomyces cerevisiae W303-1A
expression in Escherichia coli
expression in Escherichia coli as a His6-tagged fusion protein in high yield
-
expression of His-tagged enzyme in Escherichia coli
-
expression of histidine-tagged wild type Texr and mutant Texr K271R/N273D in Escherichia coli BL21-Star DE3
expression of single-site mutant xylose reductase from Candida tenuis (CtXR (K274R)) results in recombinant Corynebacterium glutamicum strain CtXR4 that produces 26.5 g/l xylitol at 3.1 g/l*h. To eliminate possible formation of toxic intracellular xylitol phosphate, genes encoding xylulokinase (XylB) and phosphoenolpyruvate-dependent fructose phosphotransferase (PTSfru) are disrupted to yield strain CtXR7
-
functional recombinant co-expression of xylose reductase (XR) from Rhizopus oryzae and glucose dehydrogenase (GDH) from Exiguobacterium sibiricum in Escherichia coli strain BL21(DE3)
gene SpXYL1.1, recombinant expression of the enzyme in Saccharomyces cerevisiae strain CEN.PK2-1C (MATa leu2-3112 ura3-52 trp1-289 his3-DELTA1 MAL2-8c SUC2), subcloning in Escherichia coli strain DH5alpha, co-expression with xylitol dehydrogenase from Spathaspora passalidarum strain UFMG-CM-Y474
-
gene xr, recombinant enzyme expression in Pichia pastoris strain GS115, coexpression with Bacillus subtilis gene gdh
gene xyl1, random multi-copy integration of the mutated KmXYL1 (mKmXYL1) gene is carried out using thermotolerant yeast Kluyveromyces marxianus strain KCTC17555DELTAURA3, in order to enhance xylitol production. After multi-copy integration, the highest xylitol producing strain is isolated and named Kluyveromyces marxianus 17555-JBP2. This strain exhibits 440% higher xylitol production than the parental strain at 30°C. Quantitative real-time PCR and transcriptome analysis demonstrate that, relative to the parent strain, Kluyveromyces marxianus strain 17555-JBP2 exhibits two more copies of mKmXY1 genes and a 9.63fold elevation in transcription of NAD(P)H-dependent XR
gene xyl1, recombinant enzyme expression in Pichia pastoris strain GS115, coexpression with Bacillus subtilis gene gdh
gene xyl1, recombinant enzyme expression in Saccharomyces cerevisiae strain CA11, isolated from Brazilian cachaca fermentation processes, co-expression with XDH, and xylA gene from Clostridium phytofermentans encoding xylose isomerase
gene xyl1, recombinant enzyme expression in Saccharomyces cerevisiae, different NAM34-4C (MATalpha) strain derivatives' genotypes and phenotypes, e.g. transformants of SCB38 with kanMX DNA or XM1, overview. XM1 is the DNA fragment carrying the genetic structure TDH3p-XYL1-TDH3t-TDH3p-XYL2-TDH3t-TDH3p-XKS1-TDH3t, including TDH3 promoter, TDH3 terminator, Scheffersomyces (Pichia) stipitis genes XYL1 (encoding NAD(P)H-dependent xylose reductase) and XYL2 (encoding NAD+-dependent xylitol dehydrogenase), and the endogenous XKS1 (encoding the Saccharomyces cerevisiae xylulokinase)
gene xyl1, recombinant expression of Xyl1 in Saccharomyces cerevisiae strain YPH499, co-expression with Aspergillus aculeatus beta-glucosidase 1 (AaBGL) and endogenous maltose transporter ScMAL11
gene xyl1, the xylose-metabolic gene XYL1 from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae
gene, recombinant expression in Saccharomyces cerevisiae strains D452-2 or EBY.VW4000, coexpression with Bacillus subtilis arabinose:H+ symporter (AraE). AraE and XYL1 expression leads to 18fold higher xylitol production compared to control strain D452-2, AraE takes up xylose efficiently and gives the engineered Saccharomyces cerevisiae strain DXXA high xylitol productivity, while the yeast wild-type shows an inefficient transport of xylose. Method optimization, overview
recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Thermochaetoides thermophila
the cofactor preference of Pichia stipitis xylose reductase is altered by site-directed mutagenesis. When the K270R xylose reductase is combined with a metabolic engineering strategy that ensures high xylose utilization capabilities, a recombinant Saccharomyces cerevisiae strain is created that provides a unique combination of high xylose consumption rate, high ethanol yield and low xylitol yield during ethanolic xylose fermentation
-