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1.1.1.315: 11-cis-retinol dehydrogenase

This is an abbreviated version!
For detailed information about 11-cis-retinol dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.315

Reaction

11-cis-retinol-[retinal-binding-protein]
+
NAD+
=
11-cis-retinal-[retinol-binding-protein]
 +
NADH
 +
H+

Synonyms

11-cis RD, 11-cis-RDH, 11-cis-retinol dehydrogenase, 11-cis-Ro-DH, atRDH, cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase, CRAD, CRAD2, cRDH, EC 5.2.1.3, RDH10, RDH5, retinol dehydrogenase 10

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.315 11-cis-retinol dehydrogenase

Engineering

Engineering on EC 1.1.1.315 - 11-cis-retinol dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA289-318
results show that the N-terminal hydrophobic domain is a membrane-anchoring domain
A294P
naturally occuring mutation, 52% of wild-type activity in cell-reporter assay, active in vitro
D128N 
naturally occuring mutation, less than 1% of wild-type activity in cell-reporter assay
D169A
mutant enzyme completely loses enzymatic activity
D169N
mutant enzyme completely loses enzymatic activity
G238W
G35S
naturally occuring mutation, 1.7% of wild-type activity in cell-reporter assay
G43A/G47A/G49A
mutant enzyme completely loses enzymatic activity
K214A
mutant enzyme completely loses enzymatic activity
K214R
mutant enzyme completely loses enzymatic activity
L105I
naturally occuring mutation, 1% of wild-type activity in cell-reporter assay
L310EV
naturally occuring mutation, 4% of wild-type activity in cell-reporter assay, no activity in vitro
R157W
naturally occuring mutation, less than 1% of wild-type activity in cell-reporter assay
R280H
naturally occuring mutation, 4% of wild-type activity in cell-reporter assay, no activity in vitro
S197A
mutation does not abolish activity
S197C
mutant enzyme completely loses enzymatic activity
S197G
mutation does not abolish activity
S197T
mutant enzyme completely loses enzymatic activity
S197V
mutant enzyme completely loses enzymatic activity
V212
naturally occuring mutation with 4bp deletion, frame shift mutant with premature stop codon at position 246
V264G
naturally occuring mutation, 4% of wild-type activity in cell-reporter assay
Y210A
mutant enzyme completely loses enzymatic activity
Y210F
mutant enzyme completely loses enzymatic activity
additional information