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additional information
additional information
-
0.00014
(S)-malate
pH 8.0, 30°C, mutant C246A
0.00015
(S)-malate
pH 7.0, 30°C, wild-type, treated with dithiothreitol
0.00017
(S)-malate
pH 8.0, 30°C, wild-type, treated with diamide
0.00039
(S)-malate
pH 8.0, 30°C, wild-type, treated with dithiothreitol
0.0008
(S)-malate
pH 7.0, 30°C, wild-type, treated with diamide
0.0017
(S)-malate
pH 8.0, 30°C, mutant C231A
0.00296
(S)-malate
-
pH 7.5, NADP-ME1
0.003
(S)-malate
pH 8.0, 30°C, mutant C192A
0.0033
(S)-malate
-
pH 7.5, NADP-ME2
0.0043
(S)-malate
pH 8.0, 30°C, mutant C270A
0.018
(S)-malate
-
60°C, pH 8.0, presence of Mn2+
0.04
(S)-malate
-
pH 7.0, wild-type C4-NADP-ME isozyme
0.05
(S)-malate
-
pH 7.4, 25°C, mutant enzyme D258A
0.065
(S)-malate
-
60°C, pH 8.0, presence of Mg2+
0.071
(S)-malate
-
pH 7.0, 60°C
0.08
(S)-malate
-
pH 7.4, 25°C, Mn2+-activated, wild-type enzyme
0.08
(S)-malate
pH 7.0, 30°C, oxidized isozyme ZmC4-NADP-ME
0.1
(S)-malate
-
pH 7.4, 25°C, mutant enzyme D235A
0.1
(S)-malate
-
pH 7.4, 25°C, mutant enzyme D257A
0.13
(S)-malate
pH 7.3, isozyme NADP-ME1
0.13
(S)-malate
-
isozyme ME1, pH 7.4, temperature not specified in the publication
0.13
(S)-malate
-
pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
0.14
(S)-malate
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME, mutant C246A
0.15
(S)-malate
pH 7.0, 30°C, reduced isozyme ZmC4-NADP-ME
0.17
(S)-malate
pH 8.0, 30°C, oxidized isozyme ZmC4-NADP-ME
0.19
(S)-malate
wild type enzyme, at pH 8.0 and 25°C
0.19
(S)-malate
-
at pH 8.0, temperature not specified in the publication
0.23
(S)-malate
-
pH 4.5, 25°C, wild-type enzyme
0.23
(S)-malate
-
pH 7.5, NADP-ME4
0.23
(S)-malate
-
pH 7.5, 30°C, isozyme NADP-ME4
0.23
(S)-malate
-
wild type isoform C4-NADP-ME, at pH 7.0 and 25°C
0.24
(S)-malate
-
recombinant ME1, pH 7.4, temperature not specified in the publication
0.27
(S)-malate
-
pH 7.4, 25°C, Mg2+-activated, wild-type enzyme
0.27
(S)-malate
-
mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.32
(S)-malate
-
isozyme ME1, pH 7.4, temperature not specified in the publication
0.32
(S)-malate
-
pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
0.36
(S)-malate
-
at pH 8.0, temperature not specified in the publication
0.39
(S)-malate
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME
0.4
(S)-malate
-
mutant E314A, with NADP+, pH 7.4, 30°C
0.4
(S)-malate
-
mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.43
(S)-malate
-
wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.44
(S)-malate
-
at pH 7.4 and 37°C
0.46
(S)-malate
-
mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C
0.47
(S)-malate
-
mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C
0.47
(S)-malate
mutant enzyme S419A, at pH 8.0 and 25°C
0.47
(S)-malate
-
mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C
0.49
(S)-malate
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.49
(S)-malate
-
isozyme ME2, pH 7.4, temperature not specified in the publication
0.49
(S)-malate
-
pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
0.51
(S)-malate
-
pH 7.0, wild-type non-C4-NADP-ME isozyme
0.58
(S)-malate
-
mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C
0.6
(S)-malate
recombinant isozyme Hvme1
0.65
(S)-malate
-
at pH 7.4 and 37°C
0.67
(S)-malate
pH 7.6, 25°C
0.68
(S)-malate
-
mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.8
(S)-malate
mutant enzyme S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G, at pH 7.4 and 30°C
0.8
(S)-malate
mutant enzyme S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/K106S/Q121S/L125H, at pH 7.4 and 30°C
0.8
(S)-malate
pH 7.4, 30°C, recombinant mutant 57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G
0.8
(S)-malate
pH 7.4, 30°C, recombinant mutant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/K106S/Q121S/L125H
0.8
(S)-malate
pH 7.4, 30°C, recombinant mutant [51-105]_c-NADP-ME
0.81
(S)-malate
-
at pH 8.0, temperature not specified in the publication
0.83
(S)-malate
-
pH 7.5, NADP-ME3
0.83
(S)-malate
-
pH 7.5, 30°C, isozyme NADP-ME3
0.84
(S)-malate
-
mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.85
(S)-malate
-
mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.89
(S)-malate
-
mutant enzyme H142A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.9
(S)-malate
-
mutant S346K, with NADP+, pH 7.4, 30°C
0.9
(S)-malate
mutant enzyme N59E, at pH 7.4 and 30°C
0.9
(S)-malate
pH 7.4, 30°C, recombinant mutant N59E
0.92
(S)-malate
-
mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.95
(S)-malate
-
mutant enzyme D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.95
(S)-malate
-
mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.97
(S)-malate
-
mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.98
(S)-malate
-
mutant enzyme W572A, in 50 mM Tris-HCl, pH 7.4, at 30°C
1
(S)-malate
-
mutant E314A/S346K/K347Y/K362H, with NAD+, pH 7.4, 30°C
1
(S)-malate
-
mutant S346K/K347Y, with NADP+, pH 7.4, 30°C
1
(S)-malate
-
recombinant isozyme PtNADP-ME1, pH 7.5, 30°C
1
(S)-malate
-
with NADP+, wild-type enzyme, pH 7.4, 30°C
1
(S)-malate
mutant enzyme S57K/N59E/E73K, at pH 7.4 and 30°C
1
(S)-malate
mutant enzyme S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/D90E/K106S/Q121S/L125H, at pH 7.4 and 30°C
1
(S)-malate
pH 7.4, 30°C, recombinant mutant S57K/N59E/E73K
1
(S)-malate
pH 7.4, 30°C, recombinant mutant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/D90E/K106S/Q121S/L125H
1.03
(S)-malate
-
mutant enzyme H51A/D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C
1.05
(S)-malate
-
mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
1.06
(S)-malate
-
activation by Mn2+
1.06
(S)-malate
-
in the presence of 10 mM Mn2+, at pH 7.4 and 25°C
1.1
(S)-malate
-
mutant enzyme H51A, in 50 mM Tris-HCl, pH 7.4, at 30°C
1.1
(S)-malate
-
recombinant isozyme PtNADP-ME5, pH 7.5, 30°C
1.12
(S)-malate
-
mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
1.2
(S)-malate
-
mutant E314A/S346K, with NADP+, pH 7.4, 30°C
1.2
(S)-malate
wild type enzyme, at pH 7.4 and 30°C
1.2
(S)-malate
mutant enzyme N59E/E73K, at pH 7.4 and 30°C
1.2
(S)-malate
mutant enzyme S57K/N59E/E73K/S102D, at pH 7.4 and 30°C
1.2
(S)-malate
pH 7.4, 30°C, recombinant mutant N59E/E73K
1.2
(S)-malate
pH 7.4, 30°C, recombinant wild-type enzyme
1.3
(S)-malate
mutant enzyme E73K, at pH 7.4 and 30°C
1.3
(S)-malate
pH 7.4, 30°C, recombinant mutant E73K
1.3
(S)-malate
pH 7.4, 30°C, recombinant mutant S57K/N59E/E73K/S102D
1.4
(S)-malate
-
recombinant isozyme PtNADP-ME4, pH 7.5, 30°C
1.4
(S)-malate
mutant enzyme N59E/E73K/S102D, at pH 7.4 and 30°C
1.4
(S)-malate
pH 7.4, 30°C, recombinant mutant N59E/E73K/S102D
1.46
(S)-malate
pH 7.0, isozyme NADP-ME2
1.5
(S)-malate
-
recombinant isozyme PtNADP-ME3, pH 7.5, 30°C
1.6
(S)-malate
mutant enzyme S102D, at pH 7.4 and 30°C
1.6
(S)-malate
pH 7.4, 30°C, recombinant mutant S102D
1.7
(S)-malate
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME, mutant C231A
1.7
(S)-malate
mutant enzyme S57K, at pH 7.4 and 30°C
1.7
(S)-malate
pH 7.4, 30°C, recombinant mutant S57K
1.8
(S)-malate
-
mutants K347Y and K362Q, with NADP+, pH 7.4, 30°C
2
(S)-malate
-
mutant E314A/S346K/K347Y/K362Q, with NAD+, pH 7.4, 30°C
2.19
(S)-malate
-
at pH 7.5 and 33°C
2.3
(S)-malate
-
isozyme ME2, pH 7.4, temperature not specified in the publication
2.3
(S)-malate
-
pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
2.6
(S)-malate
recombinant NADP-ME2
2.68
(S)-malate
mutant enzyme S419A, at pH 8.0 and 25°C
2.7
(S)-malate
-
at pH 7.0 and 30°C
2.9
(S)-malate
-
recombinant isozyme PtNADP-ME2, pH 7.5, 30°C
2.9
(S)-malate
-
in the presence of 1 mM NADP+, at pH 8.0 and 30°C
2.96
(S)-malate
-
pH 7.5, 30°C, isozyme NADP-ME1
3
(S)-malate
-
mutant E314A/S346I/K347D/K362H, with NAD+, pH 7.4, 30°C
3
(S)-malate
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME, mutant C192A
3.1
(S)-malate
recombinant NADP-ME2
3.33
(S)-malate
-
pH 7.5, 30°C, isozyme NADP-ME2
3.41
(S)-malate
-
pH 7.5, recombinant MaeB
3.63
(S)-malate
-
activation by Mg2+
3.63
(S)-malate
-
in the presence of 10 mM Mg2+, at pH 7.4 and 25°C
3.7
(S)-malate
-
mutant S346K/K362Q, with NADP+, pH 7.4, 30°C
4
(S)-malate
-
mutants E314A, S346K, E314A/S346K, S346K/K362Q, and S346K/K347Y/K362Q, with NAD+, pH 7.4, 30°C
4.3
(S)-malate
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME, mutant C270A
4.9
(S)-malate
-
mutant K362H, with NADP+, pH 7.4, 30°C
5
(S)-malate
-
mutants K347Y, K362Q, and S346K/K347Y, with NAD+, pH 7.4, 30°C
5
(S)-malate
-
with NAD+, wild-type enzyme, pH 7.4, 30°C
5.5
(S)-malate
at pH 8.0, temperature not specified in the publication
5.5
(S)-malate
-
mutant S347Y/K362Q, with NADP+, pH 7.4, 30°C
5.6
(S)-malate
-
mutant E314A/S346K/K347Y/K362Q, with NADP+, pH 7.4, 30°C
6
(S)-malate
-
mutants S346K/K347Y/K362H, with NAD+, pH 7.4, 30°C
6.4
(S)-malate
pH 8.0, 30°C, recombinant enzyme
7
(S)-malate
-
mutant S346I/K347D/K362H, with NAD+, pH 7.4, 30°C
7.3
(S)-malate
-
mutant E314A/S346K/K347Y/K362H, with NADP+, pH 7.4, 30°C
8
(S)-malate
-
mutants K362H and S347Y/K362Q, with NAD+, pH 7.4, 30°C
10
(S)-malate
-
mutant S346K/K347Y/K362Q, with NADP+, pH 7.4, 30°C
11
(S)-malate
-
mutant S346K/K347Y/K362H, with NADP+, pH 7.4, 30°C
13.33
(S)-malate
-
pH 7.4, 25°C, mutant enzyme E234A
24
(S)-malate
-
mutant E314A/S346I/K347D/K362H, with NADP+, pH 7.4, 30°C
36
(S)-malate
-
mutant S346I/K347D/K362H, with NADP+, pH 7.4, 30°C
13.3
CO2
-
-
0.04
L-malate
-
-
0.041
L-malate
Pigeon
-
pH 6.5
0.12
L-malate
-
with NADP+ as cosubstrate
0.14
L-malate
-
cytosolic enzyme
0.19
L-malate
recombinant enzyme, pH 7.0
0.22
L-malate
-
pH 7.6-7.7, 25°C
0.23
L-malate
wild-type enzyme, pH 7.0
0.23
L-malate
-
wild-type enzyme, pH 8.0, 30°C
0.26
L-malate
-
pH 8.0, 30°C, light
0.28
L-malate
-
pH 8.0, 30°C, dark
0.31
L-malate
-
mutant K435L/K436L, pH 8.0, 30°C
0.39
L-malate
Pigeon
-
pH 7.5
0.5
L-malate
mutant A392G, pH 7.0
0.6
L-malate
-
cytosolic enzyme
0.666
L-malate
-
at 1.0 mM Mn2+
0.96
L-malate
-
with NAD+ as cosubstrate
1.08
L-malate
-
at 5.0 mM Mn2+
1.1
L-malate
mutant A387G, pH 7.0
1.1
L-malate
-
pH 7.3, 30°C, isozyme 2
1.25
L-malate
-
mitochondrial enzyme
2.6
L-malate
-
mutant K225I, pH 8.0, 30°C
2.9
L-malate
mutant R237L, pH 7.0
2.9
L-malate
-
mutant R237L, pH 8.0, 30°C
3.3
L-malate
Pigeon
-
pH 8.5
0.25
NAD+
-
60°C, pH 8.0
0.9
NAD+
-
mutants E314A/S346K/K347Y/K362H and E314A/S346I/K347D/K362H, pH 7.4, 30°C
0.96
NAD+
pH 8.0, 70°C, recombinant mutant R221G/K228R
1.28
NAD+
pH 8.0, 70°C, recombinant mutant K228R
1.5
NAD+
-
mutant E314A/S346K/K347Y/K362Q, pH 7.4, 30°C
1.54
NAD+
pH 8.0, 70°C, recombinant mutant R221G/K228R/I310V
1.6
NAD+
-
mutant E314A, pH 7.4, 30°C
1.9
NAD+
-
mutant K435L/K436L, pH 8.0, 30°C
2.06
NAD+
pH 8.0, 70°C, recombinant wild-type enzyme
2.29
NAD+
pH 8.0, 70°C, recombinant mutant R221G
5
NAD+
-
mutant E314A/S346K, pH 7.4, 30°C
6
NAD+
mutant A392G, pH 7.0
6.5
NAD+
-
mutant S346I/K347D/K362H, pH 7.4, 30°C
6.55
NAD+
pH 8.0, 70°C, recombinant mutant I310V
7.1
NAD+
-
mutant S346K/K347Y/K362H, pH 7.4, 30°C
8.1
NAD+
wild-type enzyme, pH 7.0
8.1
NAD+
-
wild-type enzyme, pH 8.0, 30°C
10
NAD+
-
mutant S346K/K347Y/K362Q, pH 7.4, 30°C
11
NAD+
-
mutant K347Y, pH 7.4, 30°C
11.5
NAD+
-
wild type enzyme, in 50 mMTris-HCl (pH 7.4), at 30°C
13
NAD+
-
mutant K362Q, pH 7.4, 30°C
14
NAD+
-
mutant K362H, pH 7.4, 30°C
14
NAD+
-
mutant S346K/K362Q, pH 7.4, 30°C
17
NAD+
-
mutant S346K, pH 7.4, 30°C
18
NAD+
-
mutant S346K/K347Y, pH 7.4, 30°C
18.35
NAD+
-
wild-type enzyme, pH 7.4, 30°C
18.6
NAD+
-
in 50 mM Tris-HCl (pH 7.4)
20
NAD+
-
mutant S347Y/K362Q, pH 7.4, 30°C
0.00118
NADP+
-
cytosolic enzyme
0.00171
NADP+
pH 7.3, isozyme NADP-ME1
0.00179
NADP+
-
pH 7.4, 25°C, mutant enzyme D235A
0.0018
NADP+
-
pH 7.4, 25°C, mutant enzyme E234A
0.0018
NADP+
mutant enzyme S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/D90E/K106S/Q121S/L125H, at pH 7.4 and 30°C
0.00188
NADP+
-
mitochondrial enzyme
0.0019
NADP+
wild type enzyme, at pH 7.4 and 30°C
0.0019
NADP+
mutant enzyme S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G, at pH 7.4 and 30°C
0.002
NADP+
-
mutant E314A, pH 7.4, 30°C
0.00207
NADP+
-
pH 7.4, 25°C, Mn2+-activated, wild-type enzyme
0.0023
NADP+
mutant enzyme S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/K106S/Q121S/L125H, at pH 7.4 and 30°C
0.0026
NADP+
-
wild type enzyme, in 50 mMTris-HCl (pH 7.4), at 30°C
0.0028
NADP+
pH 7.6, 25°C
0.00283
NADP+
-
pH 7.4, 25°C, mutant enzyme D257A
0.00296
NADP+
-
pH 7.4, 25°C, mutant enzyme D258A
0.003
NADP+
-
60°C, pH 8.0
0.003
NADP+
-
mutant E314A/S346K, pH 7.4, 30°C
0.0031
NADP+
mutant enzyme S57K/N59E/E73K/S102D, at pH 7.4 and 30°C
0.0034
NADP+
-
pH 4.5, 25°C, wild-type enzyme
0.0036
NADP+
pH 7.0, 30°C, oxidized isozyme ZmC4-NADP-ME
0.0036
NADP+
pH 7.0, 30°C, wild-type, treated with diamide
0.0037
NADP+
-
mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.0039
NADP+
-
mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.004
NADP+
-
mutant enzyme H142A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.004
NADP+
-
mutant enzyme H51A/D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.0042
NADP+
-
mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.00429
NADP+
pH 7.0, isozyme NADP-ME2
0.0045
NADP+
mutant enzyme N59E/E73K/S102D, at pH 7.4 and 30°C
0.0045
NADP+
mutant enzyme S57K, at pH 7.4 and 30°C
0.0046
NADP+
mutant enzyme N59E/E73K, at pH 7.4 and 30°C
0.0048
NADP+
-
mutant enzyme D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.0049
NADP+
mutant enzyme S102D, at pH 7.4 and 30°C
0.005
NADP+
-
mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.005
NADP+
-
mutant enzyme W572A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.005
NADP+
-
wild-type enzyme, pH 7.4, 30°C
0.0053
NADP+
-
in 50 mM Tris-HCl (pH 7.4)
0.0053
NADP+
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME, mutant C246A
0.0053
NADP+
pH 8.0, 30°C, mutant C246A
0.0065
NADP+
-
pH 7.5, NADP-ME3
0.0065
NADP+
-
pH 7.5, 30°C, isozyme NADP-ME3
0.0069
NADP+
mutant enzyme S57K/N59E/E73K, at pH 7.4 and 30°C
0.0072
NADP+
-
mutant enzyme H51A, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.008
NADP+
wild-type enzyme, pH 7.0
0.008
NADP+
-
wild-type enzyme, pH 8.0, 30°C
0.008
NADP+
-
at pH 8.0, temperature not specified in the publication
0.008
NADP+
pH 8.0, 70°C, recombinant wild-type enzyme
0.0082
NADP+
-
mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C
0.0086
NADP+
recombinant enzyme, pH 7.0
0.0088
NADP+
pH 8.0, 30°C, oxidized isozyme ZmC4-NADP-ME
0.0088
NADP+
pH 8.0, 30°C, wild-type, treated with diamide
0.0089
NADP+
mutant enzyme N59E, at pH 7.4 and 30°C
0.009
NADP+
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.4, at 30°C
0.0102
NADP+
-
pH 7.5, NADP-ME4
0.0102
NADP+
-
pH 7.5, 30°C, isozyme NADP-ME4
0.0103
NADP+
-
pH 7.6-7.7, 25°C
0.011
NADP+
-
at pH 8.0, temperature not specified in the publication
0.0123
NADP+
wild type enzyme, at pH 8.0 and 25°C
0.0125
NADP+
-
pH 7.0, 60°C
0.0125
NADP+
mutant enzyme E73K, at pH 7.4 and 30°C
0.0128
NADP+
-
mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C
0.013
NADP+
-
pH 7.3, 30°C, isozyme 2
0.013
NADP+
-
mutant S346K, pH 7.4, 30°C
0.0131
NADP+
-
mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.0135
NADP+
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME, mutant C270A
0.0135
NADP+
pH 8.0, 30°C, mutant C270A
0.014
NADP+
-
activation by Mn2+
0.0147
NADP+
pH 7.0, 30°C, reduced isozyme ZmC4-NADP-ME
0.0147
NADP+
pH 7.0, 30°C, wild-type, treated with dithiothreitol
0.0149
NADP+
-
wild type isoform C4-NADP-ME, at pH 7.0 and 25°C
0.015
NADP+
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME, mutant C231A
0.015
NADP+
pH 8.0, 30°C, mutant C231A
0.015
NADP+
-
mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.0154
NADP+
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME, mutant C192A
0.0154
NADP+
pH 8.0, 30°C, mutant C192A
0.0159
NADP+
-
mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C
0.0159
NADP+
-
mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C
0.016
NADP+
Pigeon
-
pH 7.5
0.016
NADP+
-
isozyme ME2, pH 7.4, temperature not specified in the publication
0.016
NADP+
-
pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
0.019
NADP+
pH 8.0, 70°C, recombinant mutant K228R
0.021
NADP+
pH 8.0, 70°C, recombinant mutant I310V
0.023
NADP+
-
at pH 7.4 and 37°C
0.023
NADP+
-
mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.023
NADP+
-
wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.025
NADP+
-
activation by Mg2+
0.025
NADP+
pH 8.0, 70°C, recombinant mutant R221G
0.027
NADP+
-
isozyme ME1, pH 7.4, temperature not specified in the publication
0.027
NADP+
-
pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
0.0276
NADP+
pH 8.0, 30°C, reduced isozyme ZmC4-NADP-ME
0.0276
NADP+
pH 8.0, 30°C, wild-type, treated with dithiothreitol
0.03
NADP+
mutant A392G, pH 7.0
0.03
NADP+
-
mutant K347Y, pH 7.4, 30°C
0.03
NADP+
-
recombinant isozyme PtNADP-ME5, pH 7.5, 30°C
0.032
NADP+
-
isozyme ME2, pH 7.4, temperature not specified in the publication
0.032
NADP+
-
pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
0.035
NADP+
-
at pH 8.0, temperature not specified in the publication
0.036
NADP+
-
recombinant isozyme PtNADP-ME1, pH 7.5, 30°C
0.037
NADP+
mutant A387G, pH 7.0
0.038
NADP+
-
mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.04
NADP+
-
pH 7.0, wild-type C4-NADP-ME isozyme
0.04
NADP+
-
at pH 7.4 and 37°C
0.0412
NADP+
-
mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.0415
NADP+
-
pH 7.5, recombinant MaeB
0.048
NADP+
-
isozyme ME1, pH 7.4, temperature not specified in the publication
0.048
NADP+
-
pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
0.0503
NADP+
-
mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C
0.064
NADP+
-
at pH 7.0 and 30°C
0.0721
NADP+
-
pH 7.5, NADP-ME2
0.0721
NADP+
-
pH 7.5, 30°C, isozyme NADP-ME2
0.073
NADP+
-
mutant K435L/K436L, pH 8.0, 30°C
0.075
NADP+
-
recombinant isozyme PtNADP-ME4, pH 7.5, 30°C
0.079
NADP+
recombinant NADP-ME2
0.093
NADP+
recombinant NADP-ME2
0.1
NADP+
-
recombinant isozyme PtNADP-ME3, pH 7.5, 30°C
0.107
NADP+
pH 8.0, 30°C, recombinant enzyme
0.12
NADP+
-
recombinant isozyme PtNADP-ME2, pH 7.5, 30°C
0.123
NADP+
-
mutant K225I, pH 8.0, 30°C
0.14
NADP+
-
mutant S346K/K347Y, pH 7.4, 30°C
0.157
NADP+
-
at pH 8.0 and 30°C
0.1595
NADP+
mutant enzyme S419A, at pH 8.0 and 25°C
0.205
NADP+
-
pH 7.5, NADP-ME1
0.205
NADP+
-
pH 7.5, 30°C, isozyme NADP-ME1
0.244
NADP+
pH 8.0, 70°C, recombinant mutant R221G/K228R
0.29
NADP+
mutant R237L, pH 7.0
0.29
NADP+
-
mutant R237L, pH 8.0, 30°C
0.36
NADP+
-
mutant K362H, pH 7.4, 30°C
0.38
NADP+
-
at pH 7.5 and 33°C
0.384
NADP+
pH 8.0, 70°C, recombinant mutant R221G/K22P8R/I310V
0.51
NADP+
-
pH 7.0, wild-type non-C4-NADP-ME isozyme
0.76
NADP+
-
mutant K362Q, pH 7.4, 30°C
3
NADP+
-
mutant E314A/S346K/K347Y/K362H, pH 7.4, 30°C
5
NADP+
-
mutant E314A/S346K/K347Y/K362Q, pH 7.4, 30°C
6
NADP+
-
mutant S346K/K362Q, pH 7.4, 30°C
12
NADP+
-
mutant S347Y/K362Q, pH 7.4, 30°C
17
NADP+
-
mutant S346K/K347Y/K362Q, pH 7.4, 30°C
29
NADP+
-
mutants S346K/K347Y/K362H and E314A/S346I/K347D/K362H, pH 7.4, 30°C
116
NADP+
-
mutant S346I/K347D/K362H, pH 7.4, 30°C
0.0018
NADPH
pH 7.4, 30°C, recombinant mutant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/D90E/K106S/Q121S/L125H
0.0019
NADPH
pH 7.4, 30°C, recombinant mutant 57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G
0.0019
NADPH
pH 7.4, 30°C, recombinant wild-type enzyme
0.0023
NADPH
pH 7.4, 30°C, recombinant mutant S57K/N59E/E73K/S102D/H74K/D78P/D80E/D87G/K106S/Q121S/L125H
0.0027
NADPH
pH 7.4, 30°C, recombinant mutant [51-105]_c-NADP-ME
0.0031
NADPH
pH 7.4, 30°C, recombinant mutant S57K/N59E/E73K/S102D
0.0045
NADPH
pH 7.4, 30°C, recombinant mutant N59E/E73K/S102D
0.0045
NADPH
pH 7.4, 30°C, recombinant mutant S57K
0.0046
NADPH
pH 7.4, 30°C, recombinant mutant N59E/E73K
0.0049
NADPH
pH 7.4, 30°C, recombinant mutant S102D
0.0069
NADPH
pH 7.4, 30°C, recombinant mutant S57K/N59E/E73K
0.0089
NADPH
pH 7.4, 30°C, recombinant mutant N59E
0.0125
NADPH
pH 7.4, 30°C, recombinant mutant E73K
0.047
NADPH
-
at pH 7.0 and 30°C
0.5
pyruvate
pH 7.0
0.69
pyruvate
pH 7.0, isozyme NADP-ME1
1.3
pyruvate
at pH 8.0, temperature not specified in the publication
2.6
pyruvate
pH 7.0, isozyme NADP-ME2
4.3
pyruvate
-
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication
4.3
pyruvate
-
pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
5.1
pyruvate
-
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication
5.1
pyruvate
-
pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
5.3
pyruvate
-
isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication
5.3
pyruvate
-
pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+
5.8
pyruvate
-
recombinant ME1, pH 7.4, temperature not specified in the publication
6
pyruvate
-
at pH 7.0 and 30°C
7.6
pyruvate
-
isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication
7.6
pyruvate
-
pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+
10
pyruvate
-
cytosolic enzyme
16.9
pyruvate
-
pH 7.0, 30°C, isozyme NADP-ME1
18.1
pyruvate
pH 8.0, 30°C, recombinant enzyme
25
pyruvate
-
mitochondrial enzyme
26.3
pyruvate
-
pH 7.0, 30°C, isozyme NADP-ME4
48.2
pyruvate
-
pH 7.0, 30°C, isozyme NADP-ME3
138.9
pyruvate
-
pH 7.0, 30°C, isozyme NADP-ME2
additional information
additional information
-
kinetics of mutant enzymes, overview
-
additional information
additional information
-
kinetics of isozymes at different pH, overview
-
additional information
additional information
-
kinetics of recombinant chimeric mutant enzymes, overview
-
additional information
additional information
recombinant isozyme Hvme1, kinetics
-
additional information
additional information
recombinant isozyme Hvme1, kinetics
-
additional information
additional information
recombinant isozyme Hvme1, kinetics
-
additional information
additional information
-
recombinant isozyme Hvme1, kinetics
-
additional information
additional information
recombinant isozyme Hvme3, kinetics
-
additional information
additional information
recombinant isozyme Hvme3, kinetics
-
additional information
additional information
recombinant isozyme Hvme3, kinetics
-
additional information
additional information
-
recombinant isozyme Hvme3, kinetics
-
additional information
additional information
-
with malate as the variable substrate the kinetics are nonhyperbolic, exhibiting a sigmoidal response with a positive Hill coefficient
-
additional information
additional information
-
among the five isoforms, PtNADP-ME5 has the highest affinities for NADP while PtNADP-ME2 exhibits the lowest affinities toward both malate and NADP+
-
additional information
additional information
-
human m-NAD(P)-ME is a non-cooperative enzyme for substrate L-malate binding, steady-state kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetics of NADP+ and (S)-malate at different NADP+ and fumarate concentrations, overview
-