1.1.1.405: ribitol-5-phosphate 2-dehydrogenase (NADP+)

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For detailed information about ribitol-5-phosphate 2-dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

ACS1, Bcs1, bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase, ribulose-5-P reductase, ribulose-5-phosphate reductase, TarIJ, TarJ

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.405 ribitol-5-phosphate 2-dehydrogenase (NADP+)

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Results	in table
2	Activating Compound
777	AA Sequence
4	Cloned(Commentary)
2	Cofactor
1	General Information
1	General Stability
7	Inhibitors
11	kcat/KM [mM/s]
2	Ki Value [mM]
20	KM Value [mM]
6	Molecular Weight [Da]
5	Organism
5	Pathway
2	PDB ID
1	pH Optimum
2	Protein Variants
2	Purification (Commentary)
1	Reaction
5	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
13	Substrates and Products (Substrate)
3	Subunits
12	Synonyms
1	Systematic Name
14	Turnover Number [1/s]

Cloned

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Cloned on EC 1.1.1.405 - ribitol-5-phosphate 2-dehydrogenase (NADP+)

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expression in ERscherichia coli

Haemophilus influenzae

Q48154

645253

expression in Escherichia coli

Haemophilus influenzae

Q48230

645254

overexpression of TarI and TarJ individually from their respective genes using the T7 expression system

Staphylococcus aureus

650236

spr1148 gene amplified, inserted into plasmid pJFK118EH and transformed into competent cells of Escherichia coli DH5alpha. Plasmid pJFK118EH1148 isolated and insert with the spr1148 gene cut off by restriction enzymes NdeI and SacI. Fragment ligated into the overexpression plasmid pET28a(+) to generate plasmid pET1148. The plasmid transformed into Escherichia coli BL21(DE3) for overexpression of spr1148

Streptococcus pneumoniae

Q8DPI3

698603