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0.019 - 0.202
(2R,3S)-isocitrate
0.017 - 12
2-oxoglutarate
0.0017 - 0.118
D,L-isocitrate
0.028 - 0.239
D-isocitrate
0.00013 - 0.1243
DL-isocitrate
additional information
additional information
-
0.019
(2R,3S)-isocitrate
-
pH 7.6, 25°C, peroxisomes from senecent leaves
0.202
(2R,3S)-isocitrate
-
pH 7.6, 25°C, peroxisomes from young leaves
0.017
2-oxoglutarate
-
mitochondrial isozyme, reverse reaction, pH 7.0
0.026
2-oxoglutarate
-
cytosolic isozyme, reverse reaction, pH 7.0
0.04
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C
0.11
2-oxoglutarate
mutant enzyme R132A, at pH 7.5 and 37°C
0.12
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C
0.14
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 6.8 and 37°C
0.2
2-oxoglutarate
-
isozyme IDP2
0.246
2-oxoglutarate
wild type enzyme, in Tris buffer, at pH 7.5 and 37°C
0.26
2-oxoglutarate
mutant enzyme R132Q, at pH 7.5 and 37°C
0.26
2-oxoglutarate
wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C
0.26
2-oxoglutarate
wild type enzyme, in Tris buffer, at pH 7.2 and 37°C
0.28
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C
0.3
2-oxoglutarate
mutant enzyme R132G, at pH 7.5 and 21°C
0.304
2-oxoglutarate
-
pH 6.5, reverse reaction
0.34
2-oxoglutarate
mutant enzyme R132G, at pH 7.5 and 37°C
0.36
2-oxoglutarate
mutant enzyme R132C, at pH 7.5 and 21°C
0.36
2-oxoglutarate
mutant enzyme R132C, at pH 7.5 and 37°C
0.398
2-oxoglutarate
-
pH 6.5, reverse reaction
0.46
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C
0.5
2-oxoglutarate
wild type enzyme, at pH 7.5 and 37°C
0.6
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C
0.61
2-oxoglutarate
mutant enzyme R132K, at pH 7.5 and 37°C
0.65
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C
0.71
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C
0.82
2-oxoglutarate
mutant enzyme R132W, at pH 7.5 and 37°C
0.87
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C
1
2-oxoglutarate
wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C
1.1
2-oxoglutarate
-
recombinant enyme, pH 7.0
1.1
2-oxoglutarate
mutant enzyme R132H, at pH 7.5 and 37°C
1.5
2-oxoglutarate
mutant enzyme R132H, in potassium phosphate buffer, at pH 7.5 and 37°C
1.8
2-oxoglutarate
mutant enzyme R132H, at pH 7.5 and 21°C
1.92
2-oxoglutarate
-
isozyme IDP1
2.2
2-oxoglutarate
mutant enzyme R153C, at pH 7.5 and 25°C
3.3
2-oxoglutarate
mutant enzyme R153H, at pH 7.5 and 25°C
10
2-oxoglutarate
mutant enzyme R100Q, at pH 7.5 and 21°C
10
2-oxoglutarate
mutant enzyme R132N, at pH 7.5 and 37°C
12
2-oxoglutarate
mutant enzyme R100Q, at pH 7.5 and 37°C
0.2
CO2
-
mitochondrial isozyme, reverse reaction, pH 7.0
1.3
CO2
-
recombinant enyme, pH 7.0
1.6
CO2
-
cytosolic isozyme, reverse reaction, pH 7.0
11.68
CO2
-
pH 6.5, reverse reaction
13.82
CO2
-
pH 6.5, reverse reaction
0.0017
D,L-isocitrate
-
isozyme 2
0.0022
D,L-isocitrate
-
-
0.0026
D,L-isocitrate
-
-
0.0031
D,L-isocitrate
-
isozyme 1
0.0057
D,L-isocitrate
-
Mn2+-dependent reaction
0.0091
D,L-isocitrate
-
-
0.0097
D,L-isocitrate
-
25°C assay temperature
0.0125
D,L-isocitrate
-
-
0.0181
D,L-isocitrate
-
70°C assay temperature
0.0204
D,L-isocitrate
-
in presence of Mg2+
0.022
D,L-isocitrate
-
Mn2+-dependent reaction
0.059
D,L-isocitrate
-
Mg2+-dependent reaction
0.07
D,L-isocitrate
-
Mg2+-dependent reaction
0.08
D,L-isocitrate
Pinus spp.
-
-
0.028
D-isocitrate
-
Mg2+-dependent reaction, ICDH2
0.028
D-isocitrate
-
chloroplastic enzyme, at pH 7.5 and 30°C
0.04
D-isocitrate
-
recombinant enzyme, pH 8.0, 30°C
0.041
D-isocitrate
-
Mg2+-dependent reaction, ICDH1
0.041
D-isocitrate
-
cytosolic enzyme, at pH 7.5 and 30°C
0.042
D-isocitrate
pH 7.6, 25°C, leaf enzyme
0.239
D-isocitrate
pH 7.6, 25°C, root enzyme
0.00013
DL-isocitrate
-
pH 7.5, 30°C
0.0064
DL-isocitrate
mutant T311S, pH 7.4, 25°C
0.0065
DL-isocitrate
mutant R83K, pH 7.4, 25°C
0.0073
DL-isocitrate
mutant T311N, pH 7.4, 25°C
0.0083
DL-isocitrate
-
pH 7.4, 25°C, recombinant mutant H319Q
0.0084
DL-isocitrate
-
pH 7.4, 25°C, recombinant wild-type
0.0084
DL-isocitrate
wild-type, pH 7.4, 25°C
0.0087
DL-isocitrate
-
pH 7.4, 25°C, recombinant mutant H315Q
0.01
DL-isocitrate
-
isoform ICD-1, presence of Mg2+, pH 7.5, 25°C
0.011
DL-isocitrate
Q8X277
mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C
0.0114
DL-isocitrate
mutant N328D, pH 7.4, 25°C
0.014
DL-isocitrate
mutant R83Q, pH 7.4, 25°C
0.0154
DL-isocitrate
mutant N328S, pH 7.4, 25°C
0.0155
DL-isocitrate
mutant T311A, pH 7.4, 25°C
0.017
DL-isocitrate
enzyme from ischemic heart, pH 7.8, 25°C
0.018
DL-isocitrate
Q8X277
mutant R291S, pH 8.0, 30°C
0.02
DL-isocitrate
-
isoform ICD-2, presence of Mg2+, pH 7.5, 25°C
0.021
DL-isocitrate
pH and temperature not specified in the publication
0.022
DL-isocitrate
-
isoform ICD-1, presence of Zn2+, pH 7.5, 25°C
0.0326
DL-isocitrate
with Mg2+, pH 7.5, 50°C
0.0326
DL-isocitrate
with Mg2+, pH 7.5, 37°C
0.036
DL-isocitrate
Q8X277
mutant R291S/K343D/Y390P, pH 8.0, 30°C
0.036
DL-isocitrate
Q8X277
mutant R291S/Y390P, pH 8.0, 30°C
0.04
DL-isocitrate
Q8X277
mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C
0.043
DL-isocitrate
-
at pH 7.4 and 37°C
0.045
DL-isocitrate
enzyme from normoxic heart, pH 7.8, 25°C
0.054
DL-isocitrate
pH and temperature not specified in the publication
0.105
DL-isocitrate
Q8X277
recombinant, renatured enzyme, pH 8.0
0.108
DL-isocitrate
Q8X277
wild-type, pH 8.0, 30°C
0.1243
DL-isocitrate
with Mn2+, pH 7.5, 50°C
0.1243
DL-isocitrate
with Mn2+, pH 7.5, 37°C
0.0006
isocitrate
pH 7.4, mutant Y140T
0.0007
isocitrate
pH 7.4, mutant Y140E
0.001
isocitrate
-
pH 8.0, forward reaction
0.003
isocitrate
mutant enzyme K413Q, at pH 8.0 and 37°C
0.004
isocitrate
-
mitochondrial isozyme, forward reaction, pH 8.0
0.0042
isocitrate
pH 7.5, isoenzyme IDP2
0.005
isocitrate
wild type enzyme, at pH 8.0 and 37°C
0.0052
isocitrate
pH 7.4, mutant Y140F
0.0053
isocitrate
pH 7.4, mutant Y140K
0.0058
isocitrate
pH 7.5, isoenzyme IDP1
0.0059
isocitrate
-
pH 7.4, recombinant mutant Y316F
0.006
isocitrate
-
cytosolic isozyme, forward reaction, pH 8.0
0.0062
isocitrate
-
pH 7.4, recombinant mutant K323Q
0.0062
isocitrate
-
recombinant wild-type IDH1, pH not specified in the publication, temperature not specified in the publication
0.0074
isocitrate
-
pH 7.4, recombinant wild-type enzyme
0.0075
isocitrate
pH 7.4, wild-type enzyme
0.0075
isocitrate
-
recombinant wild-type enzyme, pH 7.4, 25°C
0.008
isocitrate
at pH 6.0 and 42°C
0.0082
isocitrate
recombinant enzyme, pH 7.5, 30°C
0.009
isocitrate
-
pH 8.0, forward reaction
0.009
isocitrate
pH 8.0, 55°C, cofactor: NADP+
0.0092
isocitrate
pH 7.4, mutant K212Y
0.0098
isocitrate
pH 7.4, mutant K212Q
0.01
isocitrate
-
pH 7.4, recombinant mutant K321Q
0.0127
isocitrate
-
pH 9.0, 30°C
0.014
isocitrate
-
recombinant mutant N97A, pH 7.4, 25°C
0.0147
isocitrate
-
pH 8.0, 28°C
0.015
isocitrate
pH 7.5, isoenzyme IDP3
0.015
isocitrate
wild type enzyme, at pH 7.5 and 21°C
0.01558
isocitrate
-
mutant enzyme L594E, at pH 8.0 and 20°C
0.017
isocitrate
pH 7.8, 25°C, enzyme from ischemic heart
0.02008
isocitrate
-
wild type enzyme, at pH 8.0 and 20°C
0.0217
isocitrate
mutant enzyme R153H, at pH 7.5 and 25°C
0.0242
isocitrate
wild type enzyme, at pH 7.5 and 25°C
0.027
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.2 and 37°C
0.03
isocitrate
-
isozyme IDP1
0.03
isocitrate
pH 7.5, isoenzyme IDP1
0.03
isocitrate
wild type enzyme, in Tris buffer, at pH 7.5 and 37°C
0.0304
isocitrate
mutant K256Q of isoform IDH2, at pH 7.4 and 25°C
0.0318
isocitrate
mutant enzyme R153C, at pH 7.5 and 25°C
0.03353
isocitrate
-
at pH 8.0 and 20°C
0.034
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.5 and 37°C
0.035
isocitrate
wild type enzyme, in Tris buffer, at pH 7.8 and 37°C
0.038
isocitrate
wild type enzyme, in Tris buffer, at pH 8.0 and 37°C
0.04
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 6.8 and 37°C
0.0405
isocitrate
40°C, pH 8.0
0.042
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 6.2 and 37°C
0.045
isocitrate
-
recombinant enyme, pH 9.0
0.045
isocitrate
pH 7.8, 25°C, enzyme from normoxic heart
0.04666
isocitrate
-
mutant enzyme E596L, at pH 8.0 and 20°C
0.048
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 6.5 and 37°C
0.05
isocitrate
-
isozyme IDP3
0.05
isocitrate
pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme
0.05
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.0 and 37°C
0.0505
isocitrate
in the presence of Mn2+, pH and temperature not specified in the publication
0.0505
isocitrate
pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme
0.0544
isocitrate
wild type isoform IDH2, at pH 7.4 and 25°C
0.0555
isocitrate
mutant K413Q of isoform IDH2, at pH 7.4 and 25°C
0.059
isocitrate
pH 7.5, 25°C, recombinant enzyme, with Mg2+
0.06
isocitrate
-
wild type enzyme, at pH 8.0 and 20°C
0.06
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.2 and 37°C
0.061
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.5 and 37°C
0.0654
isocitrate
in the presence of Mg2+, pH and temperature not specified in the publication
0.0654
isocitrate
pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme
0.066
isocitrate
-
recombinant mutant T78A, pH 7.4, 25°C
0.066
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 7.8 and 37°C
0.067
isocitrate
wild type enzyme, in potassium phosphate buffer, at pH 8.0 and 37°C
0.06742
isocitrate
-
mutant enzyme A741S, at pH 8.0 and 20°C
0.07
isocitrate
-
chloroplastic isozyme ICDH2, pH 7.8, 25°C
0.072
isocitrate
-
recombinant mutant N97D, pH 7.4, 25°C
0.075
isocitrate
wild type enzyme, in Bis-Tris buffer, at pH 7.0 and 37°C
0.095
isocitrate
mutant enzyme C3636S, at pH 7.5 and 25°C
0.099
isocitrate
wild type enzyme, at pH 7.5 and 25°C
0.101
isocitrate
-
recombinant mutant S95A, pH 7.4, 25°C
0.1043
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A
0.11
isocitrate
wild type enzyme, in Tris buffer, at pH 7.2 and 37°C
0.116
isocitrate
pH 7.4, mutant K212R
0.1169
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H
0.12
isocitrate
pH 7.5, 25°C, recombinant enzyme, with Mn2+
0.13
isocitrate
-
pH 8.0, 30°C, cofactor: NADP+
0.1414
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E
0.15
isocitrate
mutant enzyme K217M, in potassium phosphate buffer, at pH 7.5 and 37°C
0.18
isocitrate
-
cytosolic isozyme ICDH1, pH 7.8, 25°C
0.22
isocitrate
-
isozyme IDP2
0.22
isocitrate
pH 7.5, isoenzyme IDP2
0.22
isocitrate
wild type enzyme, at pH 7.5 and 37°C
0.232
isocitrate
-
at pH 8.2 and 18°C
0.2447
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T
0.25
isocitrate
mutant enzyme K217Q, in potassium phosphate buffer, at pH 7.5 and 37°C
0.28
isocitrate
mutant enzyme H133Q, at pH 7.5 and 21°C
0.3276
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A
0.332
isocitrate
60°C, pH 8.0
0.367
isocitrate
-
recombinant mutant T78D, pH 7.4, 25°C
0.3686
isocitrate
-
recombinant mutant R132C IDH1, pH not specified in the publication, temperature not specified in the publication
0.4
isocitrate
mutant enzyme H133Q, at pH 7.5 and 37°C
0.4341
isocitrate
-
recombinant mutant R132S IDH1, pH not specified in the publication, temperature not specified in the publication
0.5824
isocitrate
-
recombinant mutant R132H IDH1, pH not specified in the publication, temperature not specified in the publication
0.8
isocitrate
mutant enzyme R132Q, at pH 7.5 and 37°C
1.1
isocitrate
mutant enzyme R132K, at pH 7.5 and 37°C
1.5
isocitrate
mutant enzyme R132N, at pH 7.5 and 37°C
1.9
isocitrate
mutant enzyme D273L, in potassium phosphate buffer, at pH 7.5 and 37°C
2.07
isocitrate
-
recombinant mutant S95D, pH 7.4, 25°C
2.525
isocitrate
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G
2.7
isocitrate
mutant enzyme A134D, at pH 7.5 and 21°C
3.6
isocitrate
mutant enzyme R132G, at pH 7.5 and 21°C
3.6
isocitrate
mutant enzyme R132W, at pH 7.5 and 37°C
4.2
isocitrate
mutant enzyme R132H, at pH 7.5 and 37°C
5.3
isocitrate
mutant enzyme R132C, at pH 7.5 and 21°C
5.7
isocitrate
mutant enzyme R132A, at pH 7.5 and 37°C
6
isocitrate
mutant enzyme R132H, at pH 7.5 and 21°C
7
isocitrate
mutant enzyme R132G, at pH 7.5 and 37°C
8
isocitrate
mutant enzyme A134D, at pH 7.5 and 37°C
8
isocitrate
mutant enzyme R100Q, at pH 7.5 and 37°C
8.2
isocitrate
mutant enzyme R132C, at pH 7.5 and 37°C
9
isocitrate
mutant enzyme R100Q, at pH 7.5 and 21°C
12
isocitrate
mutant enzyme D273S, in potassium phosphate buffer, at pH 7.5 and 37°C
19
isocitrate
mutant enzyme D273N, in potassium phosphate buffer, at pH 7.5 and 37°C
0.012
Mg2+
-
Mg2+-dependent reaction, ICDH2
0.013
Mg2+
-
Mg2+-dependent reaction, ICDH1
0.013
Mg2+
-
recombinant enzyme, pH 8.0, 30°C
0.0239
Mg2+
-
pH 9.0, 30°C
0.12
Mg2+
-
chloroplastic isozyme ICDH2, pH 7.8, 25°C
0.125
Mg2+
-
Mg2+-dependent reaction
0.159
Mg2+
-
Mg2+-dependent reaction
0.31
Mg2+
-
cytosolic isozyme ICDH1, pH 7.8, 25°C
0.00004
Mn2+
pH 7.4, mutant Y140F
0.000084
Mn2+
pH 7.4, mutant Y140E
0.00011
Mn2+
pH 7.4, wild-type enzyme
0.00011
Mn2+
-
recombinant wild-type enzyme, pH 7.4, 25°C
0.0003
Mn2+
-
pH 7.4, recombinant wild-type enzyme
0.00033
Mn2+
-
pH 7.4, 25°C, recombinant wild-type
0.00039
Mn2+
-
pH 7.4, 25°C, recombinant mutant H319Q
0.0004
Mn2+
-
pH 7.4, recombinant mutant Y316F and K323Q
0.0004
Mn2+
-
recombinant mutant N97A, pH 7.4, 25°C
0.0005
Mn2+
-
pH 7.4, recombinant mutant K321Q
0.00091
Mn2+
pH 7.4, mutant K212Q
0.0011
Mn2+
pH 7.4, mutant K212Y
0.0024
Mn2+
-
recombinant mutant N97D, pH 7.4, 25°C
0.0027
Mn2+
-
pH 7.4, 25°C, recombinant mutant H315Q
0.0033
Mn2+
-
recombinant mutant T78A, pH 7.4, 25°C
0.004
Mn2+
-
Mn2+-dependent reaction
0.0112
Mn2+
-
recombinant mutant S95A, pH 7.4, 25°C
0.022
Mn2+
-
recombinant enzyme, pH 8.0, 30°C
0.024
Mn2+
-
Mn2+-dependent reaction
0.0288
Mn2+
-
recombinant mutant T78D, pH 7.4, 25°C
0.0668
Mn2+
-
recombinant mutant S95D, pH 7.4, 25°C
0.08
Mn2+
-
chloroplastic isozyme ICDH2, pH 7.8, 25°C
0.131
Mn2+
pH 7.4, mutant Y140K
0.21
Mn2+
-
cytosolic isozyme ICDH1, pH 7.8, 25°C
0.252
Mn2+
pH 7.4, mutant K212R
0.001
NAD+
-
wild type enzyme, at pH 8.0 and 25°C
0.0179
NAD+
wild-type, 25°C, pH 7.4
0.0207
NAD+
mutant K260R, 25°C, pH 7.4
0.0232
NAD+
mutant D375N, 25°C, pH 7.4
0.0312
NAD+
mutant K260Q, 25°C, pH 7.4
0.13
NAD+
pH 8.0, 25°C, recombinant mutant D253A/S257K/K260Q/R314D/H315I/T327A
0.144
NAD+
Q8X277
mutant R291S/K343D/Y344I/V350A/Y390P, pH 8.0, 30°C
0.959
NAD+
-
mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C
1.506
NAD+
-
mutant enzyme H590L/R601L, at pH 8.0 and 25°C
2.455
NAD+
-
wild type enzyme, at pH 8.0 and 25°C
2.62
NAD+
mutant enzyme H590L/R601D, in the presence of Mn2+, at pH 8.2 and 45°C
2.695
NAD+
-
mutant enzyme H590L/R601L, at pH 8.0 and 25°C
3.474
NAD+
mutant enzyme H590L/R601D/R650S, in the presence of Mn2+, at pH 8.2 and 45°C
3.58
NAD+
pH 8.0, 25°C, recombinant wild-type enzyme
5.534
NAD+
-
mutant enzyme K589T/H590L/R601L, at pH 8.0 and 25°C
8.49
NAD+
in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+
18.6
NAD+
in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+
47
NAD+
pH 7.5, 25°C, recombinant enzyme mutant R322D
0.000028
NADP+
-
pH 7.5, 30°C
0.00042
NADP+
pH 7.4, mutant Y140E
0.00046
NADP+
pH 7.4, mutant Y140T
0.00051
NADP+
pH 7.4, mutant Y140K
0.0012
NADP+
-
recombinant mutant N97D, pH 7.4, 25°C
0.0023
NADP+
-
pH 8.0, 28°C
0.0023
NADP+
pH 7.4, mutant Y140F
0.00242
NADP+
in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+
0.00278
NADP+
in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+
0.0029
NADP+
-
pH 9.0, 30°C
0.003
NADP+
-
mitochondrial isozyme, forward reaction, pH 8.0
0.0037
NADP+
pH 7.5, isoenzyme IDP2
0.004
NADP+
-
cytosolic isozyme, forward reaction, pH 8.0
0.0043
NADP+
mutant T311N, pH 7.4, 25°C
0.0044
NADP+
pH 7.6, 25°C, leaf enzyme
0.0049
NADP+
-
recombinant mutant N97A, pH 7.4, 25°C
0.0051
NADP+
-
pH 7.4, 25°C, recombinant mutant H319Q
0.0051
NADP+
-
recombinant mutant S95A, pH 7.4
0.0053
NADP+
-
recombinant mutant T78A, pH 7.4, 25°C
0.0055
NADP+
mutant T373S, 25°C, pH 7.4
0.0056
NADP+
-
pH 7.4, 25°C, recombinant wild-type
0.0056
NADP+
wild-type, pH 7.4, 25°C
0.0056
NADP+
pH 7.5, isoenzyme IDP1
0.006
NADP+
-
Mn2+-dependent raction
0.0062
NADP+
mutant T373A, 25°C, pH 7.4
0.0068
NADP+
-
recombinant mutant R132C IDH1, pH not specified in the publication, temperature not specified in the publication
0.007
NADP+
-
Mg2+-dependent reaction, ICDH2
0.007
NADP+
-
pH 8.0, forward reaction
0.0073
NADP+
pH 7.4, mutant K212Y
0.0073
NADP+
-
recombinant wild-type IDH1, pH not specified in the publication, temperature not specified in the publication
0.0075
NADP+
-
Mg2+-dependent reaction, ICDH1
0.0077
NADP+
-
pH 7.4, recombinant mutant K323Q
0.008
NADP+
-
pH 7.4, recombinant mutant Y316F and K321Q
0.0088
NADP+
wild-type, 25°C, pH 7.4
0.009
NADP+
-
pH 7.4, recombinant wild-type enzyme
0.0097
NADP+
mutant T311S, pH 7.4, 25°C
0.0099
NADP+
pH 7.4, wild-type enzyme
0.0099
NADP+
-
recombinant wild-type enzyme, pH 7.4, 25°C
0.01
NADP+
Pinus spp.
-
-
0.01
NADP+
pH 7.5, isoenzyme IDP3
0.0102
NADP+
-
in presence of Mg2+
0.0108
NADP+
-
recombinant mutant T78D, pH 7.4, 25°C
0.011
NADP+
mutant enzyme R153H, at pH 7.5 and 25°C
0.012
NADP+
-
Mg2+-dependent reaction
0.012
NADP+
mutant T373V, 25°C, pH 7.4
0.0121
NADP+
wild type enzyme, at pH 7.5 and 25°C
0.0122
NADP+
-
recombinant mutant R132H IDH1, pH not specified in the publication, temperature not specified in the publication
0.014
NADP+
pH 7.4, mutant K212Q
0.0144
NADP+
-
recombinant mutant R132S IDH1, pH not specified in the publication, temperature not specified in the publication
0.0148
NADP+
mutant K256Q of isoform IDH2, at pH 7.4 and 25°C
0.015
NADP+
pH 7.5, temperature not specified in the publication, recombinant His-tagged enzyme
0.015
NADP+
-
at pH 7.4 and 37°C
0.0154
NADP+
mutant K374Q 25°C, pH 7.4
0.0165
NADP+
-
25°C assay temperature
0.0165
NADP+
60°C, pH 8.0
0.0168
NADP+
wild type enzyme, in the presence of Mn2+, at pH 8.2 and 45°C
0.017
NADP+
-
pH 8.0, forward reaction
0.0176
NADP+
wild type enzyme, at pH 8.0 and 37°C
0.0176
NADP+
mutant enzyme R153C, at pH 7.5 and 25°C
0.018
NADP+
pH 7.4, mutant K212R
0.0181
NADP+
in the presence of Mg2+, pH and temperature not specified in the publication
0.0181
NADP+
pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme
0.01945
NADP+
pH 8.0, 25°C, recombinant wild-type enzyme
0.02
NADP+
-
isozyme IDP2 and IDP3
0.02
NADP+
pH 7.5, isoenzyme IDP2
0.0203
NADP+
mutant R83K, pH 7.4, 25°C
0.022
NADP+
-
at pH 8.2 and 18°C
0.0223
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H
0.0237
NADP+
-
recombinant mutant S95D, pH 7.4, 25°C
0.0246
NADP+
mutant N328D, pH 7.4, 25°C
0.0261
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A
0.027
NADP+
-
recombinant enyme, pH 9.0
0.028
NADP+
wild type enzyme, at pH 7.5 and 25°C
0.0289
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E
0.03
NADP+
-
isozyme IDP1
0.03
NADP+
pH 7.5, isoenzyme IDP1
0.03
NADP+
wild type enzyme, at pH 7.5 and 21°C
0.031
NADP+
-
70°C assay temperature
0.031
NADP+
pH 7.5, 25°C, recombinant enzyme, with Mg2+
0.032
NADP+
-
Mg2+-dependent reaction
0.032
NADP+
-
pH 7.4, recombinant mutant Y316L
0.0322
NADP+
with Mg2+, pH 7.5, 37°C
0.0324
NADP+
with Mg2+, pH 7.5, 50°C
0.0337
NADP+
wild type isoform IDH2, at pH 7.4 and 25°C
0.0378
NADP+
mutant K260R, 25°C, pH 7.4
0.0392
NADP+
40°C, pH 8.0
0.0444
NADP+
pH 8.0, 70°C
0.045
NADP+
pH and temperature not specified in the publication
0.046
NADP+
enzyme from ischemic heart, pH 7.8, 25°C
0.046
NADP+
enzyme from normoxic heart, pH 7.8, 25°C
0.046
NADP+
pH 7.8, 25°C, enzyme from ischemic heart
0.046
NADP+
pH 7.8, 25°C, enzyme from normoxic heart
0.0466
NADP+
in the presence of Mn2+, pH and temperature not specified in the publication
0.0466
NADP+
pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme
0.05
NADP+
-
Mn2+-dependent reaction
0.0552
NADP+
pH 8.0, 70°C, Km-value for NAD+ is about 140fold higher than the KM-value for NADP+
0.056
NADP+
pH and temperature not specified in the publication
0.0569
NADP+
mutant enzyme K413Q, at pH 8.0 and 37°C
0.058
NADP+
pH 7.5, 25°C, recombinant enzyme, with Mg2+
0.0616
NADP+
pH 7.6, 25°C, root enzyme
0.067
NADP+
mutant enzyme R132G, at pH 7.5 and 37°C
0.0675
NADP+
recombinant enzyme, pH 7.5, 30°C
0.07
NADP+
mutant enzyme R100Q, at pH 7.5 and 21°C
0.0717
NADP+
with Mn2+, pH 7.5, 50°C
0.0749
NADP+
mutant T311A, pH 7.4, 25°C
0.0797
NADP+
with Mn2+, pH 7.5, 37°C
0.08
NADP+
-
chloroplastic isozyme ICDH2, pH 7.8, 25°C
0.08
NADP+
wild type enzyme, at pH 7.5 and 37°C
0.0811
NADP+
mutant R83Q, pH 7.4, 25°C
0.0879
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T
0.09
NADP+
-
recombinant enzyme, pH 8.0, 30°C
0.092
NADP+
-
pH 7.4, recombinant mutant R314Q
0.093
NADP+
Q8X277
recombinant, renatured enzyme, pH 8.0
0.101
NADP+
Q8X277
wild-type, pH 8.0, 30°C
0.101
NADP+
mutant enzyme H133Q, at pH 7.5 and 21°C
0.11
NADP+
-
pH 8.0, 30°C
0.1122
NADP+
mutant K413Q of isoform IDH2, at pH 7.4 and 25°C
0.119
NADP+
mutant N328S, pH 7.4, 25°C
0.1221
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A
0.125
NADP+
-
isoform ICD-1, presence of Mg2+, pH 7.5, 25°C
0.133
NADP+
mutant D375N, 25°C, pH 7.4
0.14
NADP+
mutant enzyme R132G, at pH 7.5 and 21°C
0.16
NADP+
mutant enzyme H133Q, at pH 7.5 and 37°C
0.17
NADP+
-
cytosolic isozyme ICDH1, pH 7.8, 25°C
0.18
NADP+
mutant enzyme R100Q, at pH 7.5 and 37°C
0.184
NADP+
at pH 8.5 and 42°C
0.203
NADP+
Q8X277
mutant R291S/K343D/Y390P, pH 8.0, 30°C
0.218
NADP+
-
pH 7.4, 25°C, recombinant mutant H315Q
0.25
NADP+
mutant K260Q, 25°C, pH 7.4
0.282
NADP+
Q8X277
mutant R291S/K343D/Y344I/Y390P, pH 8.0, 30°C
0.324
NADP+
pH 8.0, 25°C, recombinant mutant D253A/S257K/K260Q/R314D/H315I/T327A
0.327
NADP+
Q8X277
mutant R291S/Y390P, pH 8.0, 30°C
0.35
NADP+
Q8X277
mutant R291S, pH 8.0, 30°C
0.4868
NADP+
mutant enzyme H590L/R601D, in the presence of Mn2+, at pH 8.2 and 45°C
0.58
NADP+
mutant enzyme R132C, at pH 7.5 and 21°C
0.7
NADP+
mutant enzyme A134D, at pH 7.5 and 21°C
0.75
NADP+
mutant enzyme R132C, at pH 7.5 and 37°C
0.8546
NADP+
pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G
1
NADP+
mutant enzyme R132H, at pH 7.5 and 21°C
1.2
NADP+
mutant enzyme A134D, at pH 7.5 and 37°C
1.6
NADP+
mutant enzyme R132H, at pH 7.5 and 37°C
10.457
NADP+
mutant enzyme H590L/R601D/R650S, in the presence of Mn2+, at pH 8.2 and 45°C
19.6
NADP+
-
isoform ICD-2, presence of Mg2+, pH 7.5, 25°C
0.00071
NADPH
mutant enzyme R153H, at pH 7.5 and 25°C
0.00072
NADPH
mutant enzyme R153C, at pH 7.5 and 25°C
0.0025
NADPH
Km less than 0.0025 mM or equal, mutant enzyme R100Q, at pH 7.5 and 21°C
0.005
NADPH
Km less than 0.005 mM or equal, mutant enzyme R132H, at pH 7.5 and 21°C
0.005
NADPH
mutant enzyme R100Q, at pH 7.5 and 37°C
0.009
NADPH
-
mitochondrial isozyme, reverse reaction, pH 7.0
0.01
NADPH
-
isozyme IDP1
0.01
NADPH
-
pH 6.5, reverse reaction
0.01
NADPH
Km less than 0.01 mM or equal, wild type enzyme, at pH 7.5 and 37°C
0.01
NADPH
mutant enzyme R132C, at pH 7.5 and 37°C
0.024
NADPH
-
cytosolic isozyme, reverse reaction, pH 7.0
0.025
NADPH
Km less than 0.025 mM or equal, mutant enzyme R132C, at pH 7.5 and 21°C
0.025
NADPH
Km less than 0.025 mM or equal, mutant enzyme R132G, at pH 7.5 and 21°C
0.025
NADPH
Km less than 0.025 mM or equal, mutant enzyme R132H, at pH 7.5 and 37°C
0.025
NADPH
Km less than 0.025 mM, mutant enzyme R132G, at pH 7.5 and 37°C
0.034
NADPH
-
pH 6.5, reverse reaction
0.04
NADPH
-
isozyme IDP2
0.56
oxalosuccinate
-
reductase
1.2
oxalosuccinate
-
decarboxylase
25 - 26
oxalosuccinate
-
decarboxylase
additional information
additional information
-
-
-
additional information
additional information
-
kinetic analysis
-
additional information
additional information
kinetic analysis
-
additional information
additional information
kinetic analysis
-
additional information
additional information
-
kinetics of isozymes IDP1 and IDP2
-
additional information
additional information
-
measurement methods, the maltose binding fusion protein of the recombinant enzymes alters the kinetic parameters, overview
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
kinetics, overview
-
additional information
additional information
kinetics, overview
-
additional information
additional information
-
kinetics, overview
-
additional information
additional information
kinetics analysis, overview
-
additional information
additional information
Km-values of wild-type and chimeric enzymes
-
additional information
additional information
-
Km-values of wild-type and chimeric enzymes
-
additional information
additional information
Km-values of wild-type and chimeric enzymes
-
additional information
additional information
-
Km-values of wild-type and chimeric enzymes
-
additional information
additional information
steady-state kinetics, kinetic mechanism of ICDH-1, overview
-
additional information
additional information
-
steady-state kinetics, kinetic mechanism of ICDH-1, overview
-
additional information
additional information
stopped-flow kinetics and steady-state kientics
-