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0.00023 - 0.00697
1-(3-phenylpropyl)-1,4-dihydropyridine-3-carboxamide
-
0.00078 - 0.009
1-benzyl-3-carbamoylpyridin-1-ium chloride
-
0.00047 - 0.042
1-phenethyl-1,4-dihydropyridine-3-carboxamide
-
1.5 - 14.2
D-glucose 6-phosphate
0.00023
1-(3-phenylpropyl)-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme V306G
-
0.00076
1-(3-phenylpropyl)-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme I192T
-
0.0009
1-(3-phenylpropyl)-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme V306I
-
0.00096
1-(3-phenylpropyl)-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, wild-type enzyme
-
0.00126
1-(3-phenylpropyl)-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme I192V
-
0.00697
1-(3-phenylpropyl)-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme I192T/V306I
-
0.00078
1-benzyl-3-carbamoylpyridin-1-ium chloride
pH and temperature not specified in the publication, mutant enzyme V306G
-
0.00089
1-benzyl-3-carbamoylpyridin-1-ium chloride
pH and temperature not specified in the publication, mutant enzyme I192T
-
0.00145
1-benzyl-3-carbamoylpyridin-1-ium chloride
pH and temperature not specified in the publication, mutant enzyme V306I
-
0.0016
1-benzyl-3-carbamoylpyridin-1-ium chloride
pH and temperature not specified in the publication, wild-type enzyme
-
0.00174
1-benzyl-3-carbamoylpyridin-1-ium chloride
pH and temperature not specified in the publication, mutant enzyme I192T/V306G
-
0.00246
1-benzyl-3-carbamoylpyridin-1-ium chloride
pH and temperature not specified in the publication, mutant enzyme I192V
-
0.009
1-benzyl-3-carbamoylpyridin-1-ium chloride
pH and temperature not specified in the publication, mutant enzyme I192T/V306I
-
0.00047
1-phenethyl-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme V306G
-
0.00254
1-phenethyl-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme 0.00184 I192T/V306G
-
0.00303
1-phenethyl-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme I192T
-
0.00396
1-phenethyl-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, wild-type enzyme
-
0.00398
1-phenethyl-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme I192V
-
0.00442
1-phenethyl-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme V306I
-
0.042
1-phenethyl-1,4-dihydropyridine-3-carboxamide
pH and temperature not specified in the publication, mutant enzyme I192T/V306I
-
7
beta-D-galactose
mutant enzyme T41V, NADP+ as cofactor
8
beta-D-galactose
mutant enzyme T41V, NAD+ as cofactor
22
beta-D-galactose
mutant enzyme T41A, NADP+ as cofactor
37
beta-D-galactose
wild type enzyme, NADP+ as cofactor
56
beta-D-galactose
mutant enzyme T41A, NAD+ as cofactor
61
beta-D-galactose
wild type enzyme, NAD+ as cofactor
4
beta-D-glucose
mutant enzyme T41V, NADP+ as cofactor
6
beta-D-glucose
mutant enzyme T41V, NAD+ as cofactor
14
beta-D-glucose
mutant enzyme T41A, NADP+ as cofactor
18
beta-D-glucose
-
E96K mutant, coenzyme NADP+
19.34
beta-D-glucose
pH 6.5, 70°C, cosubstrate: NADP+
23
beta-D-glucose
-
E96K mutant, coenzyme NAD+
39
beta-D-glucose
mutant enzyme T41A, NAD+ as cofactor
48
beta-D-glucose
wild type enzyme, NADP+ as cofactor
68.23
beta-D-glucose
pH 6.5, 70°C, cosubstrate: NAD+
75
beta-D-glucose
wild type enzyme, NAD+ as cofactor
92
beta-D-glucose
-
E96G mutant, coenzyme NADP+
97
beta-D-glucose
-
E96A mutant, coenzyme NAD+
98
beta-D-glucose
-
E96A mutant, coenzyme NADP+
114
beta-D-glucose
-
E96G mutant, coenzyme NAD+
120
beta-D-glucose
-
pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH I
140
beta-D-glucose
-
pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH II
186
beta-D-glucose
-
Y253C mutant, coenzyme NADP+
190
beta-D-glucose
-
pH 8.0, 30°C, coenzyme NAD+, isoenzymes GlcDH I and GlcDH II
200
beta-D-glucose
-
Y253C mutant, coenzyme NAD+
260
beta-D-glucose
-
wild type, coenzyme NADP+
300
beta-D-glucose
-
Q252L mutant, coenzyme NADP+
317
beta-D-glucose
-
mutant Q252L, pH 8.0, 25°C
334
beta-D-glucose
-
mutant Q252L/E170K, pH 8.0, 25°C
390
beta-D-glucose
-
wild type, coenzyme NAD+
395
beta-D-glucose
-
wild-type enzyme, pH 8.0, 25°C
420
beta-D-glucose
-
pH 8.0, 30°C, coenzyme NAD+, isoenzyme GlcDH IV
430
beta-D-glucose
-
pH 8.0, 30°C, coenzyme NAD+, isoenzyme GlcDH III
462
beta-D-glucose
-
pH 8.0, 30°C, coenzyme NADP, isoenzyme GlcDH IV
802
beta-D-glucose
-
pH 8.0, 30°C, coenzyme NADP+, isoenzyme GlcDH III
11.1
D-glucose
-
coenzyme NAD+
14.7
D-glucose
-
coenzyme NADP+
19.34
D-glucose
cosubstrate NADP+, 70°C, pH not specified in the publication
41
D-glucose
-
coenzyme NADP+
45.9
D-glucose
-
coenzyme NAD+
68.23
D-glucose
cosubstrate NAD+, 70°C, pH not specified in the publication
70
D-glucose
-
at pH 8.0 and 37°C
81.9
D-glucose
pH 7.0, 30°C, mutant enzyme Q252L/E170K/K166R
82.4
D-glucose
pH 7.0, 30°C, mutant enzyme Q252L/E170K/S100P/K166R/K137R
82.6
D-glucose
pH 7.0, 30°C, mutant enzyme Q252L/E170K/S100P
84.4
D-glucose
pH 7.0, 30°C, mutant enzyme Q252L/E170K
87.84
D-glucose
pH 8.0, 25°C
89.6
D-glucose
pH 7.0, 30°C, mutant enzyme Q252L/E170K/S100P/K166R
112
D-glucose
pH 7.0, 30°C, mutant enzyme Q252L/E170K/S100P/K166R/V72I
119
D-glucose
pH 7.0, 30°C, mutant enzyme Q252L/E170K/S100P/K166R/V72I/K137R
190
D-glucose
isozyme GlcDH-I
190
D-glucose
isozyme GlcDH-II
317
D-glucose
apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C
334
D-glucose
apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C
352
D-glucose
apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C
390
D-glucose
isozyme GlcDH-IWG3
395
D-glucose
apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C
1.5
D-glucose 6-phosphate
-
-
1.5
D-glucose 6-phosphate
-
coenzyme NADP+
1.6
D-glucose 6-phosphate
-
coenzyme NAD+
12.7
D-glucose 6-phosphate
-
coenzyme NADP+
14.2
D-glucose 6-phosphate
-
coenzyme NAD+
7
D-xylose
mutant enzyme T41V, NADP+ as cofactor
12
D-xylose
mutant enzyme T41V, NAD+ as cofactor
22
D-xylose
mutant enzyme T41A, NADP+ as cofactor
44
D-xylose
wild type enzyme, NADP+ as cofactor
61
D-xylose
wild type enzyme, NAD+ as cofactor
81
D-xylose
mutant enzyme T41A, NAD+ as cofactor
0.00008
NAD+
pH and temperature not specified in the publication, mutant enzyme I192T/V306G
0.00095
NAD+
pH and temperature not specified in the publication, mutant enzyme I192T/V306I
0.00162
NAD+
pH and temperature not specified in the publication, mutant enzyme V306G
0.00283
NAD+
pH and temperature not specified in the publication, mutant enzyme I192T
0.00286
NAD+
pH and temperature not specified in the publication, mutant enzyme I192V
0.00678
NAD+
pH and temperature not specified in the publication, wild-type enzyme
0.00838
NAD+
pH and temperature not specified in the publication, mutant enzyme V306I
54.47
NAD+
cosubstrate D-glucose, 65°C, pH not specified in the publication
61.3
NAD+
with beta-D-galactose, pH 7.5, 70°C
70.49
NAD+
cosubstrate D-glucose, 70°C, pH not specified in the publication
74.9
NAD+
with beta-D-glucose, pH 7.5, 70°C
105.4
NAD+
cosubstrate D-glucose, 80°C, pH not specified in the publication
115
NAD+
wild type enzyme, at pH 8.0 and 25°C
180
NAD+
mutant enzyme DS255, at pH 8.0 and 25°C
390
NAD+
-
wild type, coenzyme NAD+
521000
NAD+
-
at pH 8.0 and 25°C
18
NADP+
-
E96K mutant
26.74
NADP+
cosubstrate D-glucose, 65°C, pH not specified in the publication
28.52
NADP+
cosubstrate D-glucose, 70°C, pH not specified in the publication
37.4
NADP+
with beta-D-galactose, pH 7.5, 70°C
44.17
NADP+
cosubstrate D-glucose, 80°C, pH not specified in the publication
47.7
NADP+
with beta-D-glucose, pH 7.5, 70°C
93
NADP+
wild type enzyme, at pH 8.0 and 25°C
130
NADP+
mutant enzyme DS255, at pH 8.0 and 25°C