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0.374 - 35
2-deoxy-D-glucose 6-phosphate
0.279 - 0.864
6-phosphonogluconate
0.031 - 0.051
D-galactose 6-phosphate
0.0021 - 50.7
D-glucose 6-phosphate
1.5
thio-NAD+
-
pH 7.4, 80°C, recombinant enzyme
0.06
thio-NADP+
-
pH 7.4, 80°C, recombinant enzyme
additional information
additional information
-
0.374
2-deoxy-D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R454C, with NADP+
24
2-deoxy-D-glucose 6-phosphate
-
pH 7.4, 37°C, with NADP+
35
2-deoxy-D-glucose 6-phosphate
-
pH 7.4, 80°C, recombinant enzyme
0.279
6-phosphonogluconate
-
isoenzyme II
0.864
6-phosphonogluconate
-
isoenzyme II
0.031
D-galactose 6-phosphate
-
isoenzyme II
0.046 - 0.047
D-galactose 6-phosphate
-
isoenzyme I
0.046 - 0.047
D-galactose 6-phosphate
-
isoenzyme I
0.051
D-galactose 6-phosphate
-
isoenzyme II
0.0021
D-glucose 6-phosphate
-
nephridic enzyme
0.0033
D-glucose 6-phosphate
-
hepatic enzyme
0.0078
D-glucose 6-phosphate
-
wild-type enzyme, pH 7.5, 30°C
0.00953
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R454C, with NADP+
0.00971
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R454H, with NADP+
0.014
D-glucose 6-phosphate
-
25°C, pH 7.8
0.0145
D-glucose 6-phosphate
-
pH 7.5, temperature not specified in the publication
0.019
D-glucose 6-phosphate
-
pH 7.8, 85°C, recombinant enzyme
0.024
D-glucose 6-phosphate
-
-
0.026
D-glucose 6-phosphate
-
pH 8.5, 30°C
0.036
D-glucose 6-phosphate
-
pH 8.0, 25°C
0.037
D-glucose 6-phosphate
-
-
0.04
D-glucose 6-phosphate
-
-
0.04
D-glucose 6-phosphate
-
-
0.04
D-glucose 6-phosphate
-
22°C, liver extralesional parenchyma
0.041
D-glucose 6-phosphate
-
pH 7.4, 37°C
0.042
D-glucose 6-phosphate
-
cosubstrate NADP+
0.0478
D-glucose 6-phosphate
at pH 8.0 and 37°C
0.048
D-glucose 6-phosphate
-
pH 8.0, 25, mutant 2
0.0488
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R454C, with deamino-NADP+
0.0491
D-glucose 6-phosphate
-
clinical mutant G488S
0.05
D-glucose 6-phosphate
-
pH 8.0, 25°C
0.05
D-glucose 6-phosphate
-
purified enzyme, at pH 7.5 and 70°C
0.052
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant wild-type enzyme
0.052
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant wild-type enzyme, with NADP+
0.052
D-glucose 6-phosphate
-
native G6PD
0.0538
D-glucose 6-phosphate
-
clinical mutant G488V
0.0547
D-glucose 6-phosphate
-
refolded G6PD
0.056
D-glucose 6-phosphate
-
isoenzyme II
0.0578
D-glucose 6-phosphate
-
-
0.061
D-glucose 6-phosphate
-
isoenzyme I
0.063
D-glucose 6-phosphate
-
70°C, recombinant enzyme, with NADP+
0.067
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R393G
0.069
D-glucose 6-phosphate
-
recombinant from E. coli
0.07
D-glucose 6-phosphate
-
pH 7.4, 37°C
0.07
D-glucose 6-phosphate
-
pH 8.0, temperature not specified in the publication
0.072
D-glucose 6-phosphate
-
-
0.073
D-glucose 6-phosphate
-
mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 30°C
0.074
D-glucose 6-phosphate
mutant enzyme L80G, at pH 7.5 and 25°C
0.0745
D-glucose 6-phosphate
-
-
0.075
D-glucose 6-phosphate
-
pH 7.5
0.077
D-glucose 6-phosphate
-
pH 8.0, 37°C, native wild-type enzyme
0.077
D-glucose 6-phosphate
wild type enzyme, at pH 7.5 and 25°C
0.081
D-glucose 6-phosphate
-
mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 30°C
0.083
D-glucose 6-phosphate
-
pH 8.0, 37°C, recombinant wild-type enzyme
0.083
D-glucose 6-phosphate
-
pH 8.0, 25, mutant 3
0.092
D-glucose 6-phosphate
-
-
0.099
D-glucose 6-phosphate
-
mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 30°C
0.1
D-glucose 6-phosphate
-
25°C, pH 8.0, wild-type strain K-10
0.1073
D-glucose 6-phosphate
-
pH 7.6, 25°C
0.122
D-glucose 6-phosphate
-
pH 7.4, 37°C, with NADP+ or deamino-NADP+
0.138
D-glucose 6-phosphate
-
-
0.145
D-glucose 6-phosphate
-
25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82
0.15
D-glucose 6-phosphate
-
pH 7.4, 80°C, recombinant enzyme
0.15
D-glucose 6-phosphate
-
pH 8.0, 25, mutant 1
0.151
D-glucose 6-phosphate
-
mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 30°C
0.153
D-glucose 6-phosphate
-
-
0.167
D-glucose 6-phosphate
-
isoenzyme I
0.168
D-glucose 6-phosphate
mutant enzyme R408A, at pH 7.5 and 25°C
0.174
D-glucose 6-phosphate
-
25°C, pH 8.0, cofactor: NADP+
0.18
D-glucose 6-phosphate
-
isoenzyme II
0.19
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R393H
0.2
D-glucose 6-phosphate
-
22°C, normal liver parenchyma
0.206
D-glucose 6-phosphate
-
pH 7.6, temperature not specified in the publication
0.21
D-glucose 6-phosphate
pH and temperature not specified in the publication
0.23
D-glucose 6-phosphate
-
-
0.24
D-glucose 6-phosphate
-
G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars
0.25
D-glucose 6-phosphate
-
G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars
0.286
D-glucose 6-phosphate
-
pH 8.0, 37°C, recombinant mutant P409R
0.44
D-glucose 6-phosphate
-
pH 8.0, 25°C
0.5
D-glucose 6-phosphate
-
pH 8.0, 25, wild-type enzyme
0.537
D-glucose 6-phosphate
mutant enzyme K83A, at pH 7.5 and 25°C
0.57
D-glucose 6-phosphate
-
0.618
D-glucose 6-phosphate
mutant enzyme K84A, at pH 7.5 and 25°C
0.78
D-glucose 6-phosphate
-
native enzyme, pH and temperature not specified in the publication
0.86
D-glucose 6-phosphate
-
recombinant His-tagged enzyme, pH and temperature not specified in the publication
0.96
D-glucose 6-phosphate
-
25°C
1.25
D-glucose 6-phosphate
-
25°C, pH 8.0, cofactor: NAD+
1.9
D-glucose 6-phosphate
-
22°C, liver (pre)neoplastic lesions
44.4
D-glucose 6-phosphate
25°C, pH 8.0, mutant enzyme G163D
45.8
D-glucose 6-phosphate
25°C, pH 8.0, wild-type enzyme
50.7
D-glucose 6-phosphate
25°C, pH 8.0, mutant enzyme G163S
0.0156
deamino-NADP+
-
pH 8.0, 25°C, recombinant mutant R454C, with D-glucose 6-phosphate
2 - 3
deamino-NADP+
-
pH 7.4, 37°C, with D-glucose 6-phosphate
0.047
NAD+
-
-
2.477
NAD+
-
mutant enzyme K18A, at pH 8.2 and 25°C
5.09
NAD+
-
wild type enzyme, at pH 8.2 and 25°C
11.74
NAD+
-
mutant enzyme K18A/R50A, at pH 8.2 and 25°C
12
NAD+
-
pH 7.4, 80°C, recombinant enzyme
14.66
NAD+
-
mutant enzyme R50A, at pH 8.2 and 25°C
0.000012
NADP+
-
pH 8.0, 25°C
0.0003
NADP+
-
nephridic enzyme
0.001
NADP+
-
hepatic enzyme
0.0013
NADP+
-
25°C, pH 7.8
0.00238
NADP+
-
pH 8.0, 25°C, recombinant mutant R454H, with D-glucose 6-phosphate
0.00276
NADP+
-
pH 8.0, 25°C, recombinant mutant R454C, with D-glucose 6-phosphate
0.006 - 0.008
NADP+
-
isoenzymes I and II, with glucose 6-phosphate
0.006 - 0.008
NADP+
-
isoenzymes I and II, with glucose 6-phosphate
0.0066
NADP+
-
native enzyme, pH and temperature not specified in the publication
0.00694
NADP+
-
refolded G6PD
0.00707
NADP+
-
pH 8.0, 25°C, recombinant wild-type enzyme, with D-glucose 6-phosphate
0.00707
NADP+
-
native G6PD
0.0071
NADP+
-
pH 8.0, 25°C, recombinant wild-type enzyme
0.0072
NADP+
at pH 8.0 and 37°C
0.0075
NADP+
-
25°C, pH 8.0
0.0075
NADP+
-
wild type enzyme, at pH 8.2 and 25°C
0.00839
NADP+
-
pH 8.0, 25°C, recombinant mutant R454C, with deoxy-D-glucose 6-phosphate
0.0086
NADP+
-
pH 7.5, temperature not specified in the publication
0.0093
NADP+
-
pH 8.0, 25°C, recombinant mutant R393G
0.01
NADP+
-
pH 7.4, 37°C, with D-glucose 6-phosphate or 2-deoxy-D-glucose 6-phosphate
0.0107
NADP+
-
clinical mutant G488V
0.0118
NADP+
-
clinical mutant G488S
0.012
NADP+
-
recombinant from E. coli
0.0143
NADP+
-
wild-type enzyme, pH 7.5, 30°C
0.0147
NADP+
-
pH 7.4, 37°C
0.015
NADP+
-
pH 8.0, temperature not specified in the publication
0.015
NADP+
-
25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82
0.015
NADP+
-
recombinant His-tagged enzyme, pH and temperature not specified in the publication
0.0159
NADP+
-
mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 30°C
0.016
NADP+
wild type enzyme, at pH 7.5 and 25°C
0.016
NADP+
-
25°C, pH 8.0, wild-type strain K-10
0.0165
NADP+
-
pH 8.0, 25°C, recombinant mutant R393H
0.017
NADP+
-
0.0025 mM glucose 6-phosphate
0.017
NADP+
-
pH 8.0, 25°C
0.017
NADP+
mutant enzyme K84A, at pH 7.5 and 25°C
0.017
NADP+
mutant enzyme R408A, at pH 7.5 and 25°C
0.0199
NADP+
-
mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 30°C
0.02
NADP+
-
with D-glucose 6-phosphate or D-galactose 6-phosphate
0.021
NADP+
mutant enzyme K83A, at pH 7.5 and 25°C
0.0225
NADP+
-
pH 7.6, temperature not specified in the publication
0.0225
NADP+
-
mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 30°C
0.023
NADP+
-
pH 7.4, 37°C
0.024
NADP+
-
mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 30°C
0.025
NADP+
-
isoenzyme II, with 6-phosphonogluconate 0.1 mM
0.03
NADP+
-
with 2-deoxyglucose 6-phosphate, KM decreases to 10 mM with increasing substrate concentration from 0.3 to 1.5 mM
0.03
NADP+
-
pH 7.4, 80°C, recombinant enzyme
0.041
NADP+
-
G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars
0.041
NADP+
-
G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars
0.0474
NADP+
pH and temperature not specified in the publication
0.059
NADP+
-
isoform G6PD1, at pH 8.0, temperature not specified in the publication
0.06
NADP+
-
isoform G6PD1, at pH 8.0, temperature not specified in the publication
0.07
NADP+
-
pH 8.0, 25, mutant enzyme 2
0.075
NADP+
mutant enzyme L80G, at pH 7.5 and 25°C
0.077
NADP+
-
pH 8.0, 25°C
0.08
NADP+
-
pH 7.8, 85°C, recombinant enzyme
0.081
NADP+
-
at pH 8.0 temperature not specified in the publication
0.085
NADP+
-
pH 8.0, 25, mutant enzyme 3
0.099
NADP+
-
mutant enzyme K18A, at pH 8.2 and 25°C
0.116
NADP+
-
pH 8.5, 30°C
0.13
NADP+
-
pH 8.0, 25, mutant enzyme 1
0.21
NADP+
-
pH 8.0, 25, wild-type enzyme
0.382
NADP+
-
mutant enzyme R50A, at pH 8.2 and 25°C
1.98
NADP+
-
purified enzyme, at pH 7.5 and 70°C
4.67
NADP+
25°C, pH 8.0, wild-type enzyme
4.8
NADP+
25°C, pH 8.0, mutant enzyme G163D
6.46
NADP+
25°C, pH 8.0, mutant enzyme G163S
17.7
NADP+
-
mutant enzyme K18A/R50A, at pH 8.2 and 25°C
0.014
NADPH
-
recombinant from E. coli
0.023
NADPH
-
isoenzyme II, with 6-phosphonogluconate 0.1 mM
additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
Penicillium duponti
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additional information
additional information
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additional information
additional information
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kinetics
-
additional information
additional information
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kinetics and thermodynamics, rapid equilibrium random bi bi kinetic model
-
additional information
additional information
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Michaelis-Menten kinetics
-
additional information
additional information
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thermodynamics, steady-state kinetics
-
additional information
additional information
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kinetics, kinetic mechanism analysis, ternary-complex mechanism, overview
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additional information
additional information
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ping pong bi bi kinetic mechanism
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additional information
additional information
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steady-state kinetics of wild-type and mutant enzymes
-
additional information
additional information
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steady-state kinetics of wild-type and mutant enzymes
-
additional information
additional information
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hyperbolic kinetics versus D-glucose 6-phosphate
-
additional information
additional information
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the initial velocity plots of the enzyme follow the Michaelis-Menten equation in the absence of NADH. In its presence, however, the velocity versus substrate plots for NADP+ become sigmoidal but remain hyperbolic for glucose 6-phosphate as the variable substrate. Inhibition against both of the substrates of the enzyme by NADH is noncompetitive. The inhibition curves for NADH are also sigmoidal, suggesting a multisite binding of the inhibitor on the enzyme surface
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