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1.1.1.49: glucose-6-phosphate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about glucose-6-phosphate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.49

Reaction

D-glucose 6-phosphate
+
NADP+
=
6-phospho-D-glucono-1,5-lactone
+
NADPH
+
H+

Synonyms

6-phosphoglucose dehydrogenase, beta-D-glucose-6-phosphate; NADP oxidoreductase, beta-D-glucose-6-phosphate;NADP oxido-reductase, D-glucose 6-phosphate dehydrogenase, D-glucose 6-phosphate: NADP+ oxidoreductase, D-glucose-6-phosphate: NADP+ oxidoreductase, D-glucose-6-phosphate:NADP oxidoreductase, Entner-Doudoroff enzyme, G-6-PD, G-6-PDH, G-6PD, G6PD, G6PD1, G6PD2, G6PD3, G6PD4, G6PD5, G6PD6, G6PDH, G6PDH-1, G6PDH-2, G6PDH1, G6PDH2, G6PDH3, G6PDH4, G6PDH5, G6PDH6, Glc6PDH, glucose 6-phosphate dehydrogenase, glucose 6-phosphate dehydrogenase (NADP), glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase Zwf, glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, GPD, KlZWF1, NADP-dependent glucose 6-phophate dehydrogenase, NADP-glucose-6-phosphate dehydrogenase, P2-G6PDH, PF14_0511, PfGluPho, plastidial glucose 6-phosphate dehydrogenase, VEG11, Vegetative protein 11, Zwf, zwf-1, Zwf-2, Zwf1p, Zwischenferment

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.49 glucose-6-phosphate dehydrogenase (NADP+)

Temperature Stability

Temperature Stability on EC 1.1.1.49 - glucose-6-phosphate dehydrogenase (NADP+)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 30
-
activity is increased 2-3fold by heating at 25°C (either at pH 10 in 0.5 M glycine KOH buffer or at pH 5 in 0.05 M sodium citrate buffer) prior to assay, the stimulation is reversed by chilling to 0°C but is not reversed at temperatures between 20°C and 30°C
100
-
half-life: 12 h
25
-
half-life: 15 min
4
inactivation at 4°C is partially reversed at room temperature, especially with added NADP+
45
-
60 min, the wild-type enzyme is completely stable, the mutant P409R shows 86% reduced thermal stability compared to the wild-type enzyme
50
-
purified recombinant His-tagged enzyme, inactivation
60
-
t1/2 wild-type enzyme: 0.125 h. t1/2, mutant enzyme A117S/Q324H/V443I/S470I: 1.69 h. t1/2, mutant enzyme A117S/Q324H/M381I/V443I/S470I: 9.35 h. t1/2, mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I: 11.82 h. t1/2, mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V: 15.52 h
67.3
-
Tm-value, wild-type enzyme
68.8
-
Tm-value, mutant enzyme A117S/Q324H/V443I/S470I
69.6
-
Tm-value, mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I
69.7
-
Tm-value, mutant enzyme A117S/Q324H/M381I/V443I/S470I
70.7
-
Tm-value, mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V
90
-
half-life: 24 h
95 - 100
-
120 min, almost complete loss in activity
additional information