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1.1.1.8: glycerol-3-phosphate dehydrogenase (NAD+)

This is an abbreviated version!
For detailed information about glycerol-3-phosphate dehydrogenase (NAD+), go to the full flat file.

Word Map on EC 1.1.1.8

Reaction

sn-glycerol 3-phosphate
+
acceptor
=
glycerone phosphate
 +
reduced acceptor

Synonyms

(NAD+)-dependent G3pdh, (NAD+)-dependent glycerol-3-phosphate dehydrogenase, 100383152, alpha glycerophosphate dehydrogenase, alpha-glycerol phosphate dehydrogenase (NAD), alpha-glycerophosphate dehydrogenase (NAD), Cagpd1, Cagpd1p, Cagpd2, Cagpd2p, cG3PDH, CgGPD, cGPdH, cytoplasmic NAD-dependent glycerol-3-phosphate dehydrogenase, cytosolic GPDH, dehydrogenase, glycerol phosphate, DhGPD1, G-3-P dehydrogenase, G3P dehydrogenase, G3PD, G3PD1, G3PD2, G3PD3, G3PDH, GDM, GDP2, GLPD, GLY1, glycerol 1-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase, glycerol 3-phosphate dehydrogenase 1, glycerol phosphate dehydrogenase (NAD), glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase 1, glycerophosphate dehydrogenase (NAD), GPD, GPD1, Gpd1p, gpd2p, GPDH, GPDH-1, GPDH-2, GPDH1, GPDH2, GPDH3, GPDH4, GPDH5, GPDH: NAD+ 2 oxidoreductase, GPDH: NAD+ 2 oxidorreductase, GPDHc1, GRMZM2G090747_T01, GRMZM2G155348_T01, GRMZM2G173195_T01, GRMZM6G161711_T01, hydroglycerophosphate dehydrogenase, L-alpha-glycerol phosphate dehydrogenase, L-alpha-glycerophosphate dehydrogenase, L-glycerol 3-phosphate dehydrogenase, L-glycerol phosphate dehydrogenase, L-glycerophosphate dehydrogenase, mGPDH, NAD+-dependent G3P dehydrogenase, NAD+-dependent glycerol 3-phosphate dehydrogenase, NAD+-dependent glycerol-3-phosphate dehydrogenase, NAD+-GPDH, NAD-alpha-glycerophosphate dehydrogenase, NAD-dependent cG3PDH, NAD-dependent glycerol phosphate dehydrogenase, NAD-dependent glycerol-3-phosphate dehydrogenase, NAD-L-glycerol-3-phosphate dehydrogenase, NAD-linked glycerol 3-phosphate dehydrogenase, NADH-dihydroxyacetone phosphate reductase, NADP+-dependent glycerol 3-phosphate dehydrogenase, SFD1, SFD1/GLY1, ZEAMMB73_Zm00001d010670, ZEAMMB73_Zm00001d041962, ZEAMMB73_Zm00001d042152, ZmGPDH1, ZmGPDH3, ZmGPDH4, ZmGPDH5

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.8 glycerol-3-phosphate dehydrogenase (NAD+)

Purification

Purification on EC 1.1.1.8 - glycerol-3-phosphate dehydrogenase (NAD+)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 allelic forms of the enzyme
-
2 isozymes
2 isozymes with distinct isoelectric points in brain
-
3 allelic forms distinguishable by electrophoresis
-
3 isozymes in adults and 1 in larvae, distinct in isoelectric focusing
-
3 isozymes, product of the same gene mapped to left arm of chromosome II, isozyme 1: flight muscle, isozyme 3: larval and adult fat body, isozyme 2: heterodimer of 1 and 3
-
4 isozymes in testes and 5 isozymes in liver, distinct in isoelectric focusing
-
6 to 8 isozymes of bumblebee flight muscle enzyme
-
affinity chromatography
dye-affinity chromatography
-
GSTrap column chromatography
hydroxyapatite column chromatography, CM-column chromatography, and Cibacron blue affinity column chromatography
-
ion-exchange chromatography combined with affinity elution, 2 isozymes that differ in charge by analytical PAGE
-
native enzyme 50fold and 176fold from liver and muscle, respectively, by anion exchange chromatography, gel filtration, and affinity chromatography
-
native enzyme from liver cell extract of euthermic and hibernating animals 272.5-273.5fold by hydroxyapatite chromatography, buffer exchange gel filtration, cation exchange chromatography, affinity chromatography, and ultrafiltration
-
preparative isoelectric focusing
-
recombinant enzyme from Escherichia coli strain BL21 by anion exchange chromatography to over 95% purity
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
sequential affinity chromatography
-
several isozymes, distinct in isoelectric points
-