1.1.2.3: L-lactate dehydrogenase (cytochrome)
This is an abbreviated version!
For detailed information about L-lactate dehydrogenase (cytochrome), go to the full flat file.
Word Map on EC 1.1.2.3
-
1.1.2.3
-
flavin
-
heme
-
phagocyte
-
baker
-
hansenula
-
intermembrane
-
anomala
-
semiquinone
-
ferricyanide
-
flavodehydrogenase
-
lederer
-
gp91phox
-
presequence
-
p40phox
-
heme-binding
-
carbanion
-
mathews
-
flavin-binding
-
chapman
-
bromopyruvate
-
flavosemiquinone
-
analysis
-
medicine
-
environmental protection
-
synthesis
-
diagnostics
- 1.1.2.3
- flavin
- heme
- phagocyte
-
baker
- hansenula
-
intermembrane
- anomala
- semiquinone
- ferricyanide
-
flavodehydrogenase
-
lederer
- gp91phox
- presequence
-
p40phox
-
heme-binding
-
carbanion
-
mathews
-
flavin-binding
-
chapman
- bromopyruvate
-
flavosemiquinone
- analysis
- medicine
- environmental protection
- synthesis
- diagnostics
Reaction
Synonyms
Cyb2, Cyb2A, cytochrome b2, cytochrome c oxido reductase, FC b2, Fcb2, flavocytochrome b, flavocytochrome b2, iLDH, L(+)-lactate:cytochrome c oxidoreductase, L-(+)-lactate ferricytochrome c oxidoreductase, L-lactate cytochrome c oxidoreductase, L-lactate cytochrome c reductase, L-lactate dehydrogenase [Cytochrome], L-lactate ferricytochrome C oxidoreductase, L-lactate-cytochrome c oxidoreductase, L-lactate-cytochrome c-oxidoreductase, L-lactate: cytochrome c oxidoreductase, L-lactate:cytochrom c oxidoreductase, L-lactate:cytochrome c oxidoreductase, L-lactate:cytochrome c-oxidoreductase, L-LCO, L-LCR, L-LDH (FMN-dependent), lactate dehydrogenase (cytochrome), lactic acid dehydrogenase, lactic cytochrome c reductase, lldA, LldD, NAD+ - independent LDH, PA4771
ECTree
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Reference on EC 1.1.2.3 - L-lactate dehydrogenase (cytochrome)
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hatefi, Y.; Stiggall, D.L.
Metal-containing flavoprotein dehydrogenases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
Saccharomyces cerevisiae, Wickerhamomyces anomalus
-
Pritchard, G.G.
An NAD+-independent L-lactate dehydrogenase from Rhizopus oryzae
Biochim. Biophys. Acta
250
25-34
1971
Rhizopus arrhizus
Guiard, B.; Groudinsky, O.; Lederer, F.
Yeast L-lactate dehydrogenase (cytochrome b 2). Chemical characterization of the heme-binding core
Eur. J. Biochem.
34
241-247
1973
Saccharomyces cerevisiae
Jacq, C.; Lederer, F.
Cytochrome b2 from bakers yeast (L-lactate dehydrogenase). A double-headed enzyme
Eur. J. Biochem.
41
311-320
1974
Saccharomyces cerevisiae
Blazy, B.; Bardet, M.; Baudras, A.
A standard high-yield purification of L-(+)-lactate: cytochrome c oxidoreductase (cytochrome b2) from the yeast Hansenula anomala
Anal. Biochem.
88
624-633
1978
Wickerhamomyces anomalus
Labeyrie, F.; Baudras, A.; Lederer, F.
Flavocytochrome b 2 or L-lactate cytochrome c reductase from yeast
Methods Enzymol.
53
238-256
1978
Saccharomyces cerevisiae, Wickerhamomyces anomalus
Ghrir, R.; Becam, A.M.; Lederer, F.
Primary structure of flavocytochrome b2 from bakers yeast. Purification by reverse-phase high-pressure liquid chromatography and sequencing of fragment alpha cyanogen bromide peptides
Eur. J. Biochem.
139
59-74
1984
Saccharomyces cerevisiae
Black, M.T.; Gunn, F.J.; Chapman, S.K.; Reid, G.A.
Structural basis for the kinetic differences between flavocytochromes b2 from the yeasts Hansenula anomala and Saccharomyces cerevisiae
Biochem. J.
263
973-976
1989
Saccharomyces cerevisiae, Wickerhamomyces anomalus
Genet, R.; Lederer, F.
The carbanion of nitroethane is an inhibitor of, and not a substrate for, flavocytochrome b2 L-(+)-lactate dehydrogenase
Biochem. J.
266
301-304
1990
Saccharomyces cerevisiae
Lederer, F.
On some aspects of the catalysis of lactate dehydrogenation by flavocytochrome c
Flavins and Flavoproteins (Proc. Int. Symp. , 10th, Meeting Date 1990, Curti, B. , Ronchi S. , Zanetti, G. , eds. ) de Gruyter, Berlin, New York
773-782
1991
Saccharomyces cerevisiae
-
Tegoni, M.; Janot, J.M.; Labeyrie, F.
Inhibition of L-lactate: cytochrome-c reductase (flavocytochrome b2) by product binding to the semiquinone transient. Loss of reactivity towards monoelectronic acceptors
Eur. J. Biochem.
190
329-342
1990
Saccharomyces cerevisiae, Wickerhamomyces anomalus
Brunt, C.E.; Cox, M.C.; Thurgood, A.G.P.; Moore, G.R.; Reid, G.A.; Chapman, S.K.
Isolation and characterization of the cytochrome domain of flavocytochrome b2 expressed independently in Escherichia coli
Biochem. J.
283
87-90
1992
Saccharomyces cerevisiae
Silvestrini, M.C.; Tegoni, M.; Celerier, J.; Desbois, A.; Gervais, M.
Expression in Escherichia coli of the flavin and the haem domains of Hansenula anomala flavocytochrome b2 (flavodehydrogenase and b2 core) and characterization of the recombinant proteins
Biochem. J.
295
501-508
1993
Wickerhamomyces anomalus
Balme, A.; Lederer, F.
On the rate of proton exchange with solvent of the catalytic histidine in flavocytochrome b2 (yeast L-lactate dehydrogenase)
Protein Sci.
3
109-117
1994
Saccharomyces cerevisiae
Daff, S.; Manson, F.D.C.; Reid, G.A.; Chapman, S.K.
Strategic manipulation of the substrate specificity of Saccharomyces cerevisiae flavocytochrome b2
Biochem. J.
301
829-834
1994
Saccharomyces cerevisiae
-
Gaume, B.; Sharp, R.E.; Manson, F.D.C.; Chapman, S.K.; Reid, G.A.; Lederer, F.
Mutation to glutamine of histidine 373, the catalytic base of flavocytochrome b2 (L-lactate dehydrogenase)
Biochimie
77
621-630
1995
Saccharomyces cerevisiae
Tegoni, M.; Begotti, S.; Cambillau, C.
X-ray structure of two complexes of the Y143F flavocytochrome b2 mutant crystallized in the presence of lactate or phenyl lactate
Biochemistry
34
9840-9850
1995
Saccharomyces cerevisiae
Sharp, R.E.; Chapman, S.K.; Reid, G.A.
Deletions in the interdomain hinge region of flavocytochrome b2: Effects on intraprotein electron transfer
Biochemistry
35
891-899
1996
Saccharomyces cerevisiae
Daff, S.; Ingledew, W.J.; Reid, G.A.; Chapman, S.K.
New insights into the catalytic cycle of flavocytochrome b2
Biochemistry
35
6345-6350
1996
Saccharomyces cerevisiae
Sinclair, R.; Reid, G.A.; Chapman, S.K.
Re-design of Saccharomyces cerevisiae flavocytochrome b2: introduction of L-mandelate dehydrogenase activity
Biochem. J.
333
117-120
1998
Saccharomyces cerevisiae
Mowat, C.G.; Beaudoin, I.; Durley, R.C.; Barton, J.D.; Pike, A.D.; Chen, Z.W.; Reid, G.A.; Chapman, S.K.; Mathews, F.S.; Lederer, F.
Kinetic and crystallographic studies on the active site Arg289Lys mutant of flavocytochrome b2 (yeast L-lactate dehydrogenase)
Biochemistry
39
3266-3275
2000
Saccharomyces cerevisiae
Gondry, M.; Duboist, J.; Terrier, M.; Lederer, F.
The catalytic role of tyrosine 254 in flavocytochrome b2 (L-lactate dehydrogenase from baker's yeast). Comparison between the Y254F and Y254L mutant proteins
Eur. J. Biochem.
268
4918-4927
2001
Saccharomyces cerevisiae
Cunane, L.M.; Barton, J.D.; Chen, Z.W.; Welsh, F.E.; Chapman, S.K.; Reid, G.A.; Mathews, F.S.
Crystallographic study of the recombinant flavin-binding domain of baker's yeast flavocytochrome b(2): comparison with the intact wild-type enzyme
Biochemistry
41
4264-4272
2002
Saccharomyces cerevisiae (P00175)
Keyhani, J.; Keyhani, E.; Sattarahmady, N.
Expression of L-lactate dehydrogenase isoenzymes during root development in Crocus sativus L. Corm
Acta Hortic.
650
109-117
2004
Crocus sativus
-
Gaida, G.Z.; Stel'mashchuk, S.Y.; Smutok, O.V.; Gonchar, M.V.
A new method of visualization of the enzymatic activity of flavocytochrome b2 in electrophoretograms
Appl. Biochem. Microbiol.
39
221-223
2003
Ogataea angusta
-
Le, K.H.; Mayer, M.; Lederer, F.
Epitope mapping for the monoclonal antibody that inhibits intramolecular electron transfer in flavocytochrome b2
Biochem. J.
373
115-123
2003
Saccharomyces cerevisiae
Sobrado, P.; Fitzpatrick, P.F.
Solvent and primary deuterium isotope effects show that lactate CH and OH bond cleavages are concerted in Y254F flavocytochrome b2, consistent with a hydride transfer mechanism
Biochemistry
42
15208-15214
2003
Saccharomyces cerevisiae
Mowat, C.G.; Wehenkel, A.; Green, A.J.; Walkinshaw, M.D.; Reid, G.A.; Chapman, S.K.
Altered substrate specificity in flavocytochrome b2: structural insights into the mechanism of L-lactate dehydrogenation
Biochemistry
43
9519-9526
2004
Saccharomyces cerevisiae (P00175), Saccharomyces cerevisiae
Smutok, O.; Gayda, G.; Gonchar, M.; Schuhmann, W.
A novel L-lactate-selective biosensor based on flavocytochrome b2 from methylotrophic yeast Hansenula polymorpha
Biosens. Bioelectron.
20
1285-1290
2005
Ogataea angusta
Keyhani, E.; Sattarahmady, N.
Catalytic properties of three L-lactate dehydrogenases from saffron corms (Crocus sativus L.)
Mol. Biol. Rep.
29
163-166
2002
Crocus sativus
Hao, J.; Ma, C.; Gao, C.; Qiu, J.; Wang, M.; Zhang, Y.; Cui, X.; Xu, P.
Pseudomonas stutzeri as a novel biocatalyst for pyruvate production from DL-lactate
Biotechnol. Lett.
29
105-110
2007
Pseudomonas stutzeri
Smutok, O.V.; Os'mak, G.S.; Gaida, G.Z.; Gonchar, M.V.
Screening of yeasts producing stable L-lactate cytochrome c oxidoreductase and study of the regulation of enzyme synthesis
Microbiology
75
20-24
2006
Saccharomyces cerevisiae, Ogataea angusta, Saccharomyces cerevisiae IZR-106, Ogataea angusta 356, Saccharomyces cerevisiae IZR-42, Ogataea angusta K-105
-
Boubacar, A.K.; Pethe, S.; Mahy, J.P.; Lederer, F.
Flavocytochrome b2: reactivity of its flavin with molecular oxygen
Biochemistry
46
13080-13088
2007
Saccharomyces cerevisiae (P00175), Saccharomyces cerevisiae
Cenas, N.; Le, K.H.; Terrier, M.; Lederer, F.
Potentiometric and further kinetic characterization of the flavin-binding domain of Saccharomyces cerevisiae flavocytochrome b2. Inhibition by anions binding in the active site
Biochemistry
46
4661-4670
2007
Saccharomyces cerevisiae
Tsai, C.L.; Gokulan, K.; Sobrado, P.; Sacchettini, J.C.; Fitzpatrick, P.F.
Mechanistic and structural studies of H373Q flavocytochrome b2: effects of mutating the active site base
Biochemistry
46
7844-7851
2007
Saccharomyces cerevisiae (P00175)
Smutok, O.; Dmytruk, K.; Gonchar, M.; Sibirny, A.; Schuhmann, W.
Permeabilized cells of flavocytochrome b2 over-producing recombinant yeast Hansenula polymorpha as biological recognition element in amperometric lactate biosensors
Biosens. Bioelectron.
23
599-605
2007
Ogataea angusta
Mowat, C.G.; Gazur, B.; Campbell, L.P.; Chapman, S.K.
Flavin-containing heme enzymes
Arch. Biochem. Biophys.
493
37-52
2010
Saccharomyces cerevisiae (P00175)
Balme, A.; Brunt, C.E.; Pallister, R.L.; Chapman, S.K.; Reid, G.A.
Isolation and characterization of the flavin-binding domain of flavocytochrome b2 expressed independently in Escherichia coli
Biochem. J.
309
601-605
1995
Saccharomyces cerevisiae
Daff, S.; Sharp, R.E.; Short, D.M.; Bell, C.; White, P.; Manson, F.D.; Reid, G.A.; Chapman, S.K.
Interaction of cytochrome c with flavocytochrome b2
Biochemistry
35
6351-6357
1996
Saccharomyces cerevisiae
Le, K.H.D.; Boussac, A.; Frangioni, B.; Leger, C.; Lederer, F.
Interdomain contacts in flavocytochrome b2, a mutational analysis
Biochemistry
48
10803-10809
2009
Saccharomyces cerevisiae (P00175)
Shkil, H.; Stoica, L.; Dmytruk, K.; Smutok, O.; Gonchar, M.; Sibirny, A.; Schuhmann, W.
Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b2
Bioelectrochemistry
76
175-179
2009
Saccharomyces cerevisiae
Tabacchi, G.; Zucchini, D.; Caprini, G.; Gamba, A.; Lederer, F.; Vanoni, M.A.; Fois, E.
L-lactate dehydrogenation in flavocytochrome b2: a first principles molecular dynamics study
FEBS J.
276
2368-2380
2009
Saccharomyces cerevisiae (P00175)
Xia, Z.X.; Mathews, F.S.
Molecular structure of flavocytochrome b2 at 2.4 A resolution
J. Mol. Biol.
212
837-863
1990
Saccharomyces cerevisiae
Dmitruk, K.; Smutok, O.; Gonchar, M.; Sibirnyi, A.
Construction of flavocytochrome b2-overproducing strains of the thermotolerant methylotrophic yeast Hansenula polymorpha (Pichia angusta)
Microbiology
77
213-218
2008
Ogataea angusta
Sakai, H.; Nagahama, K.; Taguchi, H.; Akamatsu, T.; Morimura, S.; Kida, K.; Matsuoka, M.
Functional replacement of yeast flavocytochrome b2 with bacterial L-lactate dehydrogenase
J. Biosci. Bioeng.
110
269-272
2010
Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B/A1
Diep Le, K.H.; Lederer, F.; Golinelli-Pimpaneau, B.
Structural evidence for the functional importance of the heme domain mobility in flavocytochrome b2
J. Mol. Biol.
400
518-530
2010
Saccharomyces cerevisiae (P00175), Saccharomyces cerevisiae
Arzi, L.; Keyhani, J.; Keyhani, E.
Flavocytochrome b2 activity in Satureja hortensis L. leaves
Acta Hortic.
853
369-378
2010
Satureja hortensis
-
Smutok, O.; Broda, D.; Smutok, H.; Dmytruk, K.; Gonchar, M.
Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b2
Chemosphere
83
449-454
2011
Ogataea angusta, Ogataea angusta C-105
Lederer, F.
Another look at the interaction between mitochondrial cytochrome c and flavocytochrome b 2
Eur. Biophys. J.
40
1283-1299
2011
Saccharomyces cerevisiae
Gillet, N.; Ruiz-Pernia, J.J.; de la Lande, A.; Levy, B.; Lederer, F.; Demachy, I.; Moliner, V.
QM/MM study of l-lactate oxidation by flavocytochrome b2
Phys. Chem. Chem. Phys.
18
15609-15618
2016
Saccharomyces cerevisiae (P00175)
Engqvist, M.K.; Schmitz, J.; Gertzmann, A.; Florian, A.; Jaspert, N.; Arif, M.; Balazadeh, S.; Mueller-Roeber, B.; Fernie, A.R.; Maurino, V.G.
Glycolate oxidase3, a glycolate oxidase homolog of yeast L-lactate cytochrome c oxidoreductase, supports L-lactate oxidation in roots of Arabidopsis
Plant Physiol.
169
1042-1061
2015
Arabidopsis thaliana, Saccharomyces cerevisiae
Smutok, O.; Karkovska, M.; Smutok, H.; Gonchar, M.
Flavocytochrome b2-based enzymatic method of L-lactate assay in food products
ScientificWorldJournal
2013
461284
2013
Ogataea angusta (W1QKE8), Ogataea angusta, Ogataea angusta tr1 (W1QKE8)
Karkovska, M.; Smutok, O.; Stasyuk, N.; Gonchar, M.
L-Lactate-selective microbial sensor based on flavocytochrome b2-enriched yeast cells using recombinant and nanotechnology approaches
Talanta
144
1195-1200
2015
Ogataea angusta (W1QKE8), Ogataea angusta
Wang, Y.; Xiao, D.; Liu, Q.; Zhang, Y.; Hu, C.; Sun, J.; Yang, C.; Xu, P.; Ma, C.; Gao, C.
Two NAD-independent l-lactate dehydrogenases drive L-lactate utilization in Pseudomonas aeruginosa PAO1
Environ. Microbiol. Rep.
10
569-575
2018
Pseudomonas aeruginosa (Q9HV37), Pseudomonas aeruginosa (Q9I197)
Eprintsev, A.; Larchenkov, V.; Komarova, N.; Kovaleva, E.; Mitkevich, A.; Falaleeva, M.; Kompantseva, E.
Purification and investigation of physicochemical and regulatory properties of homogeneous L-lactate cytochrom c oxidoreductase obtained from the nonsulfur purple bacterium Rhodovulum steppense
Appl. Biochem. Microbiol.
54
370-374
2018
Rhodovulum steppense, Rhodovulum steppense A-20s
-
Yamamoto, M.; Horie, M.; Fukushima, M.; Toyotome, T.
Culture-based analysis of fungi in leaves after the primary and secondary fermentation processes during Ishizuchi-kurocha production and lactate assimilation of P. kudriavzevii
Int. J. Food Microbiol.
306
108263
2019
Pichia kudriavzevii (A0A099P7X2), Pichia kudriavzevii, Pichia kudriavzevii SD108 (A0A099P7X2)
Smutok, O.; Karkovska, M.; Serkiz, R.; Vus, D.; Cenas, N.; Gonchar, M.
A novel mediatorless biosensor based on flavocytochrome b2 immobilized onto gold nanoclusters for non-invasive L-lactate analysis of human liquids
Sens. Actuators B Chem.
250
469-475
2017
Ogataea polymorpha
-
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