1.1.2.8: alcohol dehydrogenase (cytochrome c)
This is an abbreviated version!
For detailed information about alcohol dehydrogenase (cytochrome c), go to the full flat file.
Reaction
+ = + 2 ferrocytochrome c + 2 H+
Synonyms
Ca2+-dependent PQQ-ADH, EC 1.1.99.8, EDH, ethanol dehydrogenase, exaA, exaF, PedE, PedH, PpADH, PP_2674, PP_2679, PQQ-ADH, PQQ-alcohol dehydrogenase, PQQ-dependent alcohol dehydrogenase, PQQ-dependent type I alcohol dehydrogenase, PQQ-DH9, pyrroloquinoline quinone ethanol dehydrogenase, pyrroloquinoline quinone-dependent alcohol dehydrogenases, pyrroloquinoline quinone-dependent dehydrogenase, pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase, pyrroquinoline quinone-dependent alcohol dehydrogenase, quinoprotein alcohol dehydrogenase
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Substrates Products
Substrates Products on EC 1.1.2.8 - alcohol dehydrogenase (cytochrome c)
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REACTION DIAGRAM
1,2-butanediol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
1,2-cyclopentandiol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
1,3-butanediol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
1-butanol + phenazine methosulfate
butanal + reduced phenazine methosulfate
-
-
-
?
1-cyclohexylethanol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
1-hexanol + phenazine methosulfate
hexanal + reduced phenazine methosulfate
-
-
-
?
1-octanol + phenazine methosulfate
octanal + reduced phenazine methosulfate
-
-
-
?
2,4-pentanediol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
2-butanol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
2-butanol + phenazine methosulfate
butan-2-one + reduced phenazine methosulfate
-
-
-
?
2-hexanol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
2-phenylethanol + phenazine methosulfate
phenylacetaldehyde + reduced phenazine methosulfate
butyraldehyde + phenazine methosulfate
? + reduced phenazine methosulfate
-
-
-
?
cinnamyl alcohol + phenazine methosulfate
cinnamyl aldehyde + reduced phenazine methosulfate
-
-
-
?
cis-1,2-cyclohexanediol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
cyclohexanol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-arabinose + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-arabitol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-galactose + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-gluconate + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-lyxose + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-mannitol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-ribose + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-sorbitol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
D-xylose + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine
ethanal + ?
-
i.e. Wurster's Blue
-
-
?
ethanol + oxidized 2,6-dichlorophenolindophenol
ethanal + reduced 2,6-dichlorophenolindophenol
ethanol + phenazine methosulfate
acetaldehyde + reduced phenazine methosulfate
-
-
-
?
ethanolamine + phenazine methosulfate
? + reduced phenazine methosulfate
-
-
-
?
farnesol + phenazine methosulfate
farnesal + reduced phenazine methosulfate
-
-
-
?
glyceraldehyde + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
glycerol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
hexanal + phenazine methosulfate
? + reduced phenazine methosulfate
-
-
-
?
isopropanol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
L-arabinose + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
L-ribose + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
meso-erythritol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
methanol + phenazine methosulfate
formaldehyde + reduced phenazine methosulfate
-
-
-
?
octanal + phenazine methosulfate
? + reduced phenazine methosulfate
-
-
-
?
ribitol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
threitol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
trans-1,2-cyclohexanediol + phenazine methosulfate
? + reduced phenazine methosulfate
Gluconobacter sp. CHM43
-
-
-
-
?
2,3-butanediol + phenazine methosulfate
? + reduced phenazine methosulfate
-
-
-
?
phenylacetaldehyde + reduced phenazine methosulfate
-
-
-
?
2-phenylethanol + phenazine methosulfate
phenylacetaldehyde + reduced phenazine methosulfate
converted with 76% of the activity determined with ethanol
-
-
?
? + reduced phenazine methosulfate
-
-
-
?
acetaldehyde + phenazine methosulfate
? + reduced phenazine methosulfate
converted with 71% of the activity determined with ethanol
-
-
?
butyraldehyde + 2 reduced cytochrome c
-
-
-
r
butanol + 2 cytochrome c
butyraldehyde + 2 reduced cytochrome c
-
-
-
r
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
-
-
?
diethylstilbestrol + pyrroloquinoline quinone
diethylstilbestrol 4-semiquinone + pyrroloquinoline quinol
-
i.e. 4,4'-(3E)-hex-3-ene-3,4-diyldiphenol
-
-
?
acetaldehyde + 2 reduced cytochrome c
-
-
-
-
r
ethanol + 2 cytochrome c
acetaldehyde + 2 reduced cytochrome c
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-
-
r
ethanol + 2 cytochrome c
acetaldehyde + 2 reduced cytochrome c
-
-
-
r
ethanol + 2 cytochrome c
acetaldehyde + 2 reduced cytochrome c
-
-
-
r
ethanal + 2 reduced cytochrome c
-
EPR-study to elucidate reaction mechanism. In an addition/elimination mechanism, the negatively charged substrate oxygen then performs a nucleophilic addition to the PQQ(C5) to form a covalent substrate-PQQ complex. This is followed by elimination of ethanal, leaving the fully reduced PQQH2. In a hydride transfer mechanism, a nucleophilic addition to the PQQ(C5) again occurs, but this time it is the hydride from C1 of the substrate that is transferred, completing the oxidization of the ethanol to ethanal. Subsequently, the PQQ enolizes to form PQQH2. The results are consistent with either proposed mechanism
-
-
?
ethanol + 2 cytochrome c
ethanal + 2 reduced cytochrome c
-
-
-
-
?
ethanal + 2 ferrocytochrome c
-
-
-
-
?
ethanol + 2 ferricytochrome c
ethanal + 2 ferrocytochrome c
-
-
-
?
ethanol + 2 ferricytochrome c
ethanal + 2 ferrocytochrome c
-
-
-
?
ethanol + 2 ferricytochrome c
ethanal + 2 ferrocytochrome c
-
-
-
?
ethanol + 2 ferricytochrome c
ethanal + 2 ferrocytochrome c
-
-
-
-
?
ethanol + 2 ferricytochrome c
ethanal + 2 ferrocytochrome c
-
-
-
?
ethanal + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
ethanol + oxidized 2,6-dichlorophenolindophenol
ethanal + reduced 2,6-dichlorophenolindophenol
-
-
-
?
formaldehyde + 2 reduced cytochrome c
-
-
-
-
r
methanol + 2 cytochrome c
formaldehyde + 2 reduced cytochrome c
-
the maximal reaction velocity of ExaF-La for formaldehyde is 23% greater than for methanol as the substrate
-
-
r
methanol + 2 cytochrome c
formaldehyde + 2 reduced cytochrome c
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-
-
r
methanol + 2 cytochrome c
formaldehyde + 2 reduced cytochrome c
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
the maximal reaction velocity of ExaF-La for formaldehyde is 23% greater than for methanol as the substrate
-
-
r
propanol + 2 cytochrome c
propanal + 2 reduced cytochrome c
-
-
-
-
?
?
-
Gluconobacter sp. CHM43
-
the enzyme oxidizes a wide variety of secondary alcohols but has higher KM-values than PQQ-dependent glycerol dehydrogenase with regard to linear substrates such as glycerol. Cyclic substrates such as cis-1,2-cyclohexanediol are readily oxidized
-
-
-
additional information
?
-
-
ExaF is functionally an ethanol dehydrogenase, with secondary activities with formaldehyde, methanol, and acetaldehyde. Enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol. Methanol-independent reduction of the latter is not observed with purified enzyme
-
-
?
additional information
?
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
ExaF is functionally an ethanol dehydrogenase, with secondary activities with formaldehyde, methanol, and acetaldehyde. Enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol. Methanol-independent reduction of the latter is not observed with purified enzyme
-
-
?
additional information
?
-
-
enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol
-
-
?
additional information
?
-
-
enzyme assays are performed with with the artificial electron acceptors phenazine methosulfate and 2,6-dichlorophenolindophenol
-
-
?