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10
(R)-1-phenyl-1,2-ethanediol
-
pH 7.5, 30°C
86
(S)-1-phenyl-1,2-ethanediol
-
pH 7.5, 30°C
170
1,2,4-butanetriol
-
pH 7.5, 30°C
150
1,2-Butanediol
-
pH 7.5, 30°C
97
1,2-hexanediol
-
pH 7.5, 30°C
52
1,2-pentanediol
-
pH 7.5, 30°C
3.33
1,4-benzoquinone
-
pH 5.5, 35°C
83
1-phenyl-1,2-ethanediol
-
pH 7.5, 30°C
0.0368
2,6-dichloroindophenol
-
-
35
2-amino-1-pentanol
-
pH 7.5, 30°C
8.3 - 49.8
2-deoxy-D-glucose
250
3-butene-1,2-diol
-
pH 7.5, 30°C
480
3-butenol
-
pH 7.5, 30°C
42
4-pentene-1,2-diol
-
pH 7.5, 30°C
0.019 - 733
beta-D-glucose
36
D-mannitol
-
pH 7.5, 30°C
1.4
D-sorbitol
-
pH 7.5, 30°C
0.19
di-(2,2'-bipyridinyl)ruthenium(III)dichloride
-
pH 7.3, 30°C
0.0638 - 0.1107
ferrocinium-methanol
350
glycerol
-
pH 7.5, 30°C
430
L-arabinose
-
pH 7.5, 30°C
25
L-Threitol
-
pH 7.5, 30°C
2.9 - 7
methyl-1,4-benzoquinone
2.43
phenazine methosulfate
-
pH 4.7
0.32
xylitol
-
pH 7.5, 30°C
0.694
[(1,10-phenanthroline)2(Cl)2Ru(III)]
-
pH 7.3, 30°C
0.019
[(1,8-dimethyl-4,5-phenanthroline)3Ru(II)]PF6-
-
pH 7.3, 30°C
0.52
[(2,2'-(4,4'dimethyl)bipyridine)2(Cl)2Ru(III)]
-
pH 7.3, 30°C
0.0313
[(2,2'-(4,4'dimethyl)bipyridine)2(Cl)2Ru(III)]PF6-
-
pH 7.3, 30°C
0.0922
[(2,2'-bipyridine)2(CO32-)1/2Ru(III)]
-
pH 7.3, 30°C
0.153
[(2,2'-bipyridine)2(H2O)2Ru(III)]PF6-
-
pH 7.3, 30°C
0.0513
[(2,2'-bipyridine)2(SCN-)2Ru(III)]
-
pH 7.3, 30°C
0.057
[(2,2'-bipyridine)3Ru(II)]PF6-
-
pH 7.3, 30°C
additional information
additional information
-
8.3
2-deoxy-D-glucose
-
recombinant enzyme
28.1 - 49.8
2-deoxy-D-glucose
-
pH 5.0, 25°C
0.019
beta-D-glucose
-
native enzyme in solution, pH 5.5, temperature not specified in the publication
0.149
beta-D-glucose
immobilized enzyme, pH and temperature not specified in the publication
1.51 - 3.4
beta-D-glucose
-
depending on O2-concentration, comparison of values with enzyme immobilized on various materials
1.6
beta-D-glucose
-
64°C, pH not specified in the publication
1.9
beta-D-glucose
-
0.05 M Tris buffer, pH 8
1.9
beta-D-glucose
-
purified enzyme under argon, in 20 mM phosphate buffer pH 7.4 at 37°C
2
beta-D-glucose
-
0.1 M Tris buffer, pH 8
2 - 4
beta-D-glucose
25°C, pH 6.0, mutant enzyme Y73F
2 - 14
beta-D-glucose
-
pH 3.45, 35°C
2.1
beta-D-glucose
-
second polyethyleneimine/GOD bilayer
2.5
beta-D-glucose
-
non-purified enzyme under argon, in 20 mM phosphate buffer pH 7.4 at 37°C
2.5
beta-D-glucose
-
recombinant wild-type enzyme, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme
2.6
beta-D-glucose
-
recombinant mutant K424E, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme
2.9
beta-D-glucose
-
first polyethyleneimine/GOD bilayer
3.2
beta-D-glucose
-
recombinant mutant K424E, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme
3.4
beta-D-glucose
-
recombinant mutant K424I, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme
3.8
beta-D-glucose
-
recombinant wild-type enzyme, pH 7.0, 37°C, with ferrocenemethanol, immobilized enzyme
4
beta-D-glucose
-
immobilized enzyme, immobilized membrane with ratio stretching 1.25
4 - 5.4
beta-D-glucose
-
immobilized enzyme
4.4
beta-D-glucose
-
0.6 M sodium acetate buffer, pH 6
4.5
beta-D-glucose
-
immobilized enzyme, methanol treated
5
beta-D-glucose
-
0.1 M sodium acetate buffer, pH 5
5.2
beta-D-glucose
-
0.1 M sodium acetate buffer, pH 6
5.4
beta-D-glucose
-
immobilized enzyme, immobilized membrane with ratio stretching 3
5.7
beta-D-glucose
-
native enzyme
5.8
beta-D-glucose
25°C, pH 6.0, mutant enzyme H520A
6.2
beta-D-glucose
-
recombinant enzyme
6.2
beta-D-glucose
25°C, pH 6.0, wild-type enzyme
6.3
beta-D-glucose
-
deglycosylated enzyme
6.3
beta-D-glucose
-
wild-type enzyme conjugated to gold nanoparticles, pH and temperature not specified in the publication
6.4
beta-D-glucose
-
0.1 M sodium phosphate buffer, pH 7
6.7
beta-D-glucose
-
0.1 M potassium phosphate buffer, pH 7
6.7
beta-D-glucose
25°C, pH 6.0, mutant enzyme H520V
7.1
beta-D-glucose
-
0.1 M sodium acetate buffer, pH 4.5
7.9
beta-D-glucose
-
randomly mixed polyethyleneimine/GOD
8
beta-D-glucose
-
enzyme adsorbed on 11-(1H-pyrol-11-(1H-pyrol-1-yl)undecane-1-thiol) coated matrix, high enzyme concentration, pH 5.5, temperature not specified in the publication
8.1
beta-D-glucose
-
0.1 M potassium phosphate buffer, pH 6
8.2
beta-D-glucose
-
mutant H447C conjugated to gold nanoparticles, pH and temperature not specified in the publication
10.5
beta-D-glucose
-
in 50 mM sodium acetate buffer (pH 5.4). at 45°C
11.43
beta-D-glucose
recombinant mutant M12 expressed in Pichia pastoris, pH 7.4, 25°C
11.7
beta-D-glucose
-
recombinant enzyme yGOXpenag, using O2 as cosubstrate, pH 6.0, 50°C
11.9
beta-D-glucose
-
purified enzyme under O2, in 20 mM phosphate buffer pH 7.4 at 37°C
12
beta-D-glucose
-
enzyme adsorbed on 11-amino-1-undecanethiol coated matrix, pH 5.5, temperature not specified in the publication
12.42
beta-D-glucose
-
in the presence of 0.6 M trehalose, at 25°C
13.3
beta-D-glucose
25°C, pH 6.0, mutant enzyme F418V
13.33
beta-D-glucose
recombinant mutant M12 enzyme expressed in Pichia pastoris, pH 5.5, 25°C
14.7 - 15.3
beta-D-glucose
-
pH 5.0, 25°C
14.98
beta-D-glucose
pH 5.5, temperature not specified in the publication, mutant enzyme T30V/I94V/A162T
15
beta-D-glucose
-
mutant H447C, pH and temperature not specified in the publication
16
beta-D-glucose
recombinant enzyme mutant B11, pH 5.5, 25°C
16
beta-D-glucose
pH 5.5, 25°C, mutant enzyme B11-GOx
16.5
beta-D-glucose
-
0.1 M sodium acetate buffer, pH 4
16.95
beta-D-glucose
recombinant enzyme, in 0.1 M sodium phosphate buffer, pH 6.0, at 35°C
17.5
beta-D-glucose
pH 5.1, 37°C, unmodified enzyme
18.1
beta-D-glucose
recombinant mutant M12 enzyme expressed in Saccharomyces cerevisiae, pH 5.5, 25°C
18.2
beta-D-glucose
-
recombinant enzyme yGOXpenag, using ferrocinium-methanol as cosubstrate, pH 6.0, 50°C
18.4
beta-D-glucose
-
pH 7.0, 25°C
18.54
beta-D-glucose
pH 5.5, temperature not specified in the publication, mutant enzyme T30V/I94V/A162T/R537K/M556V
18.76
beta-D-glucose
-
in the absence of trehalose, at 25°C
19.76
beta-D-glucose
pH 5.5, temperature not specified in the publication, mutant enzyme T30V/R37K/I94V/V106I/A162T/M556V
22
beta-D-glucose
-
soluble enzyme
22
beta-D-glucose
pH 5.5, 25°C, wild-type enzyme
22
beta-D-glucose
-
enzyme adsorbed on 11-(1H-pyrol-11-(1H-pyrol-1-yl)undecane-1-thiol) coated matrix, low enzyme concentration, pH 5.5, temperature not specified in the publication
22
beta-D-glucose
-
recombinant mutant K424I, pH 7.0, 37°C, with Os-(tpy)(MeCOOH-bpy)Cl2, immobilized enzyme
22
beta-D-glucose
recombinant wild-type enzyme expressed in Saccharomyces cerevisiae, pH 5.5, 25°C
22
beta-D-glucose
recombinant wild-type enzyme, pH 5.5, 25°C
22.5
beta-D-glucose
-
0.6 M sodium acetate buffer, pH 4.5
23.19
beta-D-glucose
recombinant wild-type enzyme expressed in Pichia pastoris, pH 7.4, 25°C
23.2
beta-D-glucose
pH 5.1, 37°C, aniline-modified enzyme
23.7
beta-D-glucose
-
BTL wild-type strain enzyme
25.2
beta-D-glucose
-
free enzyme
26 - 30
beta-D-glucose
-
enzymes obtained from different companies
27
beta-D-glucose
25°C, pH 6.0, mutant enzyme W430A
27.9
beta-D-glucose
recombinant enzyme mutant B11 in fusion with Aga2, pH 5.5, 25°C
28
beta-D-glucose
-
periodate-oxidized enzyme
28.26
beta-D-glucose
recombinant wild-type enzyme expressed in Pichia pastoris, pH 5.5, 25°C
28.26
beta-D-glucose
pH 5.5, temperature not specified in the publication, wild-type enzyme
28.8
beta-D-glucose
recombinant free enzyme, pH 6.0, 30°C
29.7
beta-D-glucose
recombinant immobilized enzyme, pH 6.0, 30°C
30
beta-D-glucose
-
native enzyme
30
beta-D-glucose
pH 6.0, 25°C, glycosylated enzyme
31.8
beta-D-glucose
-
commercially available enzyme
32.4
beta-D-glucose
pH 5.1, 37°C, benzoate-modified enzyme
33
beta-D-glucose
-
soluble enzyme
33
beta-D-glucose
pH 6.0, 25°C, deglycosylated enzyme
33.4
beta-D-glucose
recombinant wild-type enzyme in fusion with Aga2, pH 5.5, 25°C
34.9
beta-D-glucose
-
at pH 8.2, 40°C
35
beta-D-glucose
-
deglycosylated enzyme
36
beta-D-glucose
25°C, pH 6.0, mutant enzyme N518T
37
beta-D-glucose
-
native enzyme
37 - 38
beta-D-glucose
-
-
37 - 38
beta-D-glucose
-
-
38
beta-D-glucose
-
carbohydrate-depleted enzyme
38.1
beta-D-glucose
-
at pH 8.2, 50°C
44
beta-D-glucose
-
immobilized enzyme
44.9
beta-D-glucose
-
at pH 8.2, 60°C
50
beta-D-glucose
-
pH 6.01, 35°C
50.3
beta-D-glucose
-
native enzyme, using O2 as cosubstrate, pH 6.5, 70°C
53.24
beta-D-glucose
-
pH 4.5, 60°C
55.2
beta-D-glucose
-
at pH 8.2, 70°C
65.7
beta-D-glucose
pH 6.0, 30°C
67
beta-D-glucose
-
pH 4.82, 35°C
69
beta-D-glucose
pH 7.0, 20-25°C, mutant enzyme Y169C/A211C
71.2
beta-D-glucose
-
native enzyme, using ferrocinium-methanol as cosubstrate, pH 6.5, 70°C
72
beta-D-glucose
-
immobilized enzyme
78
beta-D-glucose
25°C, pH 6.0, mutant enzyme F418
87
beta-D-glucose
-
pH 4.52, 35°C
89.13
beta-D-glucose
-
pH 6.0, 37°C
89.13
beta-D-glucose
-
pH and temperature not specified in the publication
96.4
beta-D-glucose
-
wild-type enzyme, pH and temperature not specified in the publication
103
beta-D-glucose
pH 7.0, 20-25°C, wild-type enzyme
106
beta-D-glucose
-
pH 4.27, 35°C
130
beta-D-glucose
-
pH 3.88, 35°C
513
beta-D-glucose
25°C, pH 6.0, mutant enzyme R516K
537
beta-D-glucose
-
pH 2.84, 35°C
733
beta-D-glucose
25°C, pH 6.0, mutant enzyme R516Q
76.9 - 126.3
D-galactose
-
pH 5.0, 25°C
952
D-galactose
-
recombinant enzyme
12.5
D-glucose
-
-
13.5
D-glucose
pH 5.0, 28°C, native enzyme
15.25
D-glucose
pH 5.0, 28°C, recombinant enzyme
15.6
D-glucose
-
pH 5.0, 25°C, enzyme fraction 1
21.9
D-glucose
-
pH 5.0, 25°C, enzyme fraction 2
25
D-glucose
-
pH 5.5, 40°C
26
D-glucose
-
native enzyme
55.5
D-maltose
-
-
57.3
D-maltose
-
pH 5.0, 25°C
44
D-mannose
-
-
106
D-mannose
-
recombinant enzyme
33
D-xylose
-
-
42.9 - 70.7
D-xylose
-
pH 5.0, 25°C
384
D-xylose
-
recombinant enzyme
0.0638
ferrocinium-methanol
-
recombinant enzyme yGOXpenag, pH 6.0, 50°C
0.1107
ferrocinium-methanol
-
native enzyme, pH 6.5, 70°C
2.9
methyl-1,4-benzoquinone
-
low pH, 35°C
3.25
methyl-1,4-benzoquinone
-
pH 2.84, 35°C
3.97
methyl-1,4-benzoquinone
-
pH 3.45, 35°C
4.98
methyl-1,4-benzoquinone
-
pH 3.88, 35°C
5.7
methyl-1,4-benzoquinone
-
pH 4.27, 35°C
6.22
methyl-1,4-benzoquinone
-
pH 4.52, 35°C
6.63
methyl-1,4-benzoquinone
-
pH 4.82, 35°C
6.94
methyl-1,4-benzoquinone
-
pH 6.01, 35°C
7
methyl-1,4-benzoquinone
-
pH 7, 35°C
0.18
O2
-
periodate-oxidized enzyme
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
values for glyco-enzyme and aglyco-enzyme
-
additional information
additional information
-
KM-value of immunoaffinity-layered glucose oxidase preparations remains unaltered
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
thermodynamics
-
additional information
additional information
-
Michaelis-Menten kinetics, overview
-
additional information
additional information
-
kinetics of enzyme-pentacyanoferrate(III)-nucleophilic ligands-complex interactions, detailed overview
-
additional information
additional information
-
steady-state kinetics, overview
-
additional information
additional information
-
choline, glucose, myo-inositol, methanol, ethanol, 1-pentanol, benzyl alcohol, 2-phenylethanol, cholesterol or lauryl alcohol tested as potential substrates, recombinant protein devoid of either oxidase or dehydrogenase activity
-
additional information
additional information
-
substrate specificity and steady state kinetics, overview
-
additional information
additional information
-
enzyme thermodynamics and kinetics
-
additional information
additional information
Michaelis-Menten kinetics of immobilized enzyme GOx and free enzyme GOx
-
additional information
additional information
kinetic limitations of a bioelectrochemical electrode using carbon nanotube-attached glucose oxidase for biofuel cells. Carbon nanotube-supported glucose oxidase is examined in the presence of 1,4-benzoquinone. The intrinsic Michaelis parameters of the reaction catalyzed by carbon nanotube-glucose-oxidase are very close to those of native enzyme. However, the Nafion entrapment of carbon nanotube-glucose-oxidase for an electrode results in a much lower activity due to the limited availability of the embedded enzyme. Kinetic studies reveal that the biofuel cell employing such an enzyme electrode only generate a power density equivalent to less than 40% of the reaction capability of the enzyme on electrode. Factors such as electron and proton transfer resistances can be more overwhelming than the heterogeneous reaction kinetics in limiting the power generation of such biofuel cells
-