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1.1.5.2: glucose 1-dehydrogenase (PQQ, quinone)

This is an abbreviated version!
For detailed information about glucose 1-dehydrogenase (PQQ, quinone), go to the full flat file.

Word Map on EC 1.1.5.2

Reaction

D-glucose
+
ubiquinone
=
D-glucono-1,5-lactone
+
ubiquinol

Synonyms

(PQQ)GDH, aldose sugar dehydrogenase, Asd, beta-D-glucose:(acceptor) 1-oxidoreductase, D-glucose:(pyrroloquinoline-quinone) 1-oxidoreductase, dehydrogenase, glucose (pyrroloquinoline-quinone), EC 1.1.99.17, GCD, GDH, GDH-B, GDH1, GdhA, GDHm, GlcDH, glucose dehydrogenase, glucose dehydrogenase (PQQ dependent), glucose dehydrogenase (pyrroloquinoline-quinone), glucose dehydrogenase Amano 5, m-GDH, membrane glucose dehydrogenase, membrane-bound glucose dehydrogenase, membrane-bound PQQ-dependent glucose dehydrogenase, mGDH, PQQ GDH, PQQ glucose dehydrogenase, PQQ-dependent GDH, PQQ-dependent glucose dehydrogenase, PQQ-dependent mGDH, PQQ-dependent soluble glucose dehydrogenase, PQQ-GDH, PQQ-glucose dehydrogenase, PQQ-linked GCD, PQQ-sGDH, PqqC, PQQGDH, PQQGDH-B, pyrroloquinoline quinone dependent glucose dehydrogenase, pyrroloquinoline quinone glucose dehydrogenase, pyrroloquinoline quinone-dependent glucose dehydrogenase, QGDH, quinone-dependent glucose dehydrogenase, quinoprotein aldose sugar dehydrogenase, quinoprotein D-glucose dehydrogenase, quinoprotein glucose dehydrogenase, quinoprotein glucose DH, s-GDH, sGDH, sGDH-PQQ, soluble glucose dehydrogenase, soluble PQQ-dependent glucose dehydrogenase, soluble quinoprotein (PQQ-containing) glucose dehydrogenase, Tt_ASD, water-soluble PQQ glucose dehydrogenase, water-soluble pyrroquinoline quinone glucose dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.5 With a quinone or similar compound as acceptor
                1.1.5.2 glucose 1-dehydrogenase (PQQ, quinone)

Cloned

Cloned on EC 1.1.5.2 - glucose 1-dehydrogenase (PQQ, quinone)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli AT15 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli NovaBlue (DE3) cells
-
expression in Escherichia coli BL21, wild-type and mutant enzymes Y156A and Y156K
expression in the periplasm of Escherichia coli strain PP2418, with and without a C-terminal tag of 3 Arg residues
expression of isozyme PQQGDH-B with and without an polyarginine tail in Escherichia coli strain PP2418, and of mutant H168Q
-
expression of isozyme PQQGDH-B, in Pichia pastoris using the alpha-factor signal sequence of Saccharomyces cerevisiae, recombinant enzyme is secreted to the medium, optimization of enzyme production
-
expression of the apoenzyme in Escherichia coli
-
expression of wild-type and mutant isozyme PQQGDH-B
expression of wild-type and mutant isozymes PQQGDH-B
-
expression of wild-type isozyme PQQGDH-B and mutant enzymes in Escherichia coli
GDH1 gene is homologously expressed in Pseudomonas taetrolens or heterologously expressed in Escherichia coli. Recombinant expression of the GDH1 gene enhances intracellular lactose oxidizing activity and lactobionic acid production of Pseudomonas taetrolens in flask culture
gene gdhB, expression of the enzyme GDH-B as fusion protein C-terminally fused to cytochrome c domain from Comamonas testosteroni in Escherichia coli DH5alpha
-
gene gdhS, expressionin Escherichia coli strain S17-1
-
gene gox0265 or mgdh, overexpression of Strep-tagged membrane-bound glucose dehydrogenase from vector pBBR1p452 in membranes of an enzyme-deficient Gluconobacter oxydans strain, that shows 9fold higher enzyme expression level and 5fold increased mGDH activity, and 3fold increased oxygen consumption rates, compared to the wild-type strain, gluconate production and analysis of whole cells, overview
-
into a His-tagged vector for expression in Escherichia coli JM109
into the pET M-11 vector for transformation of DH5alpha and BL21DE3 cells
-
mutants are expressed in Escherichia coli YU423 cells
-
operon qqqABCDEF, gene gcd
random peptide ligands M13-phage library display is used to expresss the enzyme in presence of peptide ligands, overview
-
recombinant high-level production of soluble His-tagged pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli strain BL21 (DE3), 6fold improvement improvement of sPQQGDH expression in MMBL medium
-