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1.1.5.9: glucose 1-dehydrogenase (FAD, quinone)

This is an abbreviated version!
For detailed information about glucose 1-dehydrogenase (FAD, quinone), go to the full flat file.

Word Map on EC 1.1.5.9

Reaction

D-glucose
+
a quinone
=
D-glucono-1,5-lactone
 +
a quinol

Synonyms

D-glucose:acceptor 1-oxidoreductase, dehydrogenase, glucose (acceptor), dehydrogenase, glucose (Aspergillus), EC 1.1.99.10, FAD dependent glucose dehydrogenase, FAD glucose dehydrogenase, FAD-dependent GDH, FAD-dependent glucose dehydrogenase, FAD-GDH, FAD-glucose dehydrogenase, FAD-glucose dehydrogenases, FADGDH, FADGH, flavin adenine dinucleotide dependent glucose dehydrogenase, flavin adenine dinucleotide-dependent glucose dehydrogenase, GDH, GDH-FAD, GLD, glucose dehydrogenase, glucose dehydrogenase (Aspergillus), glucose dehydrogenase (decarboxylating), thermostable GDH, thermostable glucose dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.5 With a quinone or similar compound as acceptor
                1.1.5.9 glucose 1-dehydrogenase (FAD, quinone)

Engineering

Engineering on EC 1.1.5.9 - glucose 1-dehydrogenase (FAD, quinone)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H505A
the mutant enzyme shows drastic decrease in the enzymatic activity
H548A
the mutant enzyme shows drastic decrease in the enzymatic activity
V149C/G190C
the mutant shows a 110 min half-life of thermal inactivation at 45°C, which is 13fold greater than that of the wild type enzyme
H505A
-
the mutant enzyme shows drastic decrease in the enzymatic activity
-
H548A
-
the mutant enzyme shows drastic decrease in the enzymatic activity
-
A472C
-
site directed mutagenesis
A472D
-
site directed mutagenesis
A472E
-
site directed mutagenesis
A472F
A472G
-
site directed mutagenesis
A472H
-
site directed mutagenesis
A472I
-
site directed mutagenesis
A472K
-
site directed mutagenesis
A472L
-
site directed mutagenesis
A472M
-
site directed mutagenesis
A472N
-
site directed mutagenesis
A472P
-
site directed mutagenesis
A472Q
-
site directed mutagenesis
A472R
-
site directed mutagenesis
A472S
-
site directed mutagenesis
A472T
-
site directed mutagenesis
A472V
-
site directed mutagenesis
A472W
-
site directed mutagenesis
A472Y
-
site directed mutagenesis
C212S
-
inactive
C213S
-
the mutant shows 68% of wild type activity
C218S
-
inactive
C222S
-
inactive
N475A
-
site directed mutagenesis
N475C
-
site directed mutagenesis
N475D
N475E
-
site directed mutagenesis
N475F
-
site directed mutagenesis
N475G
-
site directed mutagenesis
N475H
-
site directed mutagenesis
N475I
-
site directed mutagenesis
N475K
-
site directed mutagenesis
N475L
-
site directed mutagenesis
N475M
-
site directed mutagenesis
N475P
-
site directed mutagenesis
N475Q
-
site directed mutagenesis
N475R
-
site directed mutagenesis
N475S
-
site directed mutagenesis
N475T
-
site directed mutagenesis
N475V
-
site directed mutagenesis
N475W
-
site directed mutagenesis
N475Y
-
site directed mutagenesis
S326C
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S326E
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S326G
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S326H
-
the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme
S326K
-
the mutant shows increased activity toward D-glucose compared to the wild type enzyme
S326Q
-
the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme
S326Q/S365Y
-
the mutant is virtually non-reactive to maltose while retaining high D-glucose dehydrogenase activity
S326R
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S326T
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S326V
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S326Y
-
the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme
S365A
-
the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme
S365C
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365D
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365E
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365F
-
the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme
S365G
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365H
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365I
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365K
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365L
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365M
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365N
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365P
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365Q
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365R
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365T
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365V
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365W
-
the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme
S365Y
S365Y/S326E
-
the mutant shows reduced activity compared to the wild type enzyme
S365Y/S326G
-
the mutant shows reduced activity compared to the wild type enzyme
S365Y/S326H
-
the mutant shows reduced activity compared to the wild type enzyme
S365Y/S326K
-
the mutant shows reduced activity compared to the wild type enzyme
S365Y/S326Q
-
the mutant shows reduced activity compared to the wild type enzyme
S365Y/S326R
-
the mutant shows reduced activity compared to the wild type enzyme
S365Y/S326T
-
the mutant shows reduced activity compared to the wild type enzyme
S365Y/S326V
-
the mutant shows reduced activity compared to the wild type enzyme
additional information