1.1.99.14: glycolate dehydrogenase
This is an abbreviated version!
For detailed information about glycolate dehydrogenase, go to the full flat file.
Word Map on EC 1.1.99.14
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1.1.99.14
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glyoxylate
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photorespiration
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photorespiratory
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d-lactate
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phosphoglycolate
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glycerate
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microbodies
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hydroxypyruvate
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carboligase
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tartronic
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glcdh
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high-co2-requiring
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14coxalate
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leaf-type
- 1.1.99.14
- glyoxylate
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photorespiration
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photorespiratory
- d-lactate
- phosphoglycolate
- glycerate
- microbodies
- hydroxypyruvate
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carboligase
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tartronic
- glcdh
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high-co2-requiring
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14coxalate
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leaf-type
Reaction
Synonyms
GADH, GDH, GlcD, GlcD1, GlcD2, GlcDH, glycolate dehydrogenase, glycolate dehydrogenase I, glycolate oxidoreductase, glycolic acid dehydrogenase, More
ECTree
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Substrates Products
Substrates Products on EC 1.1.99.14 - glycolate dehydrogenase
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REACTION DIAGRAM
2,6-dichlorophenol-indophenol + acceptor
?
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DCIP decoloration assay, mitochondrial glycolate oxidation contributes to photorespiration in higher plants
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?
D-lactate + 2,6-dichlorophenolindophenol
?
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100% activity with D-lactate and 2,6-dichlorophenolindophenol
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?
D-lactate + phenazine methosulfate
?
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93% activity compared to 2,6-dichlorophenolindophenol
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?
DL-malate + acceptor
2-oxosuccinate + reduced acceptor
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about 3% activity of glycolate
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-
?
glycerate 3-phosphate + 2,6-dichlorophenolindophenol
?
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2.9% activity compared to D-lactate
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-
?
glycolaldehyde + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
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38% activity compared to glycolate
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-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
glycolate + nitro blue tetrazolium
glyoxylate + reduced nitro blue tetrazolium
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100% activity
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?
L-lactate + 2,6-dichlorophenolindophenol
?
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12.4% activity compared to D-lactate
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-
?
L-lactate + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
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232% activity compared to glycolate
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-
?
methyl glycolate + nitro blue tetrazolium
methyl glyoxylate + reduced nitro blue tetrazolium
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82% activity compared to glycolate
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-
?
phosphoglycolate + acceptor
? + reduced acceptor
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about 9% activity of glycolate
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?
succinate + 2,6-dichlorophenolindophenol
?
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8.5% activity compared to D-lactate
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?
(R)-lactate + acceptor
pyruvate + reduced acceptor
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artificial acceptor 2,6-dichlorophenolindophenol is reduced via phenazine methosulfate, cytochrome c can replace 2,6-dichlorophenolindophenol
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-
?
pyruvate + reduced acceptor
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about 16% activity of glycolate
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-
?
? + reduced nitro blue tetrazolium
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162% activity compared to glycolate
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?
dihydroxyacetone + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
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162% activity compared to glycolate
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-
?
? + reduced nitro blue tetrazolium
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78% activity compared to glycolate
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-
?
DL-glyceraldehyde + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
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78% activity compared to glycolate
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-
?
? + reduced nitro blue tetrazolium
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235% activity compared to glycolate
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-
?
DL-lactate + nitro blue tetrazolium
? + reduced nitro blue tetrazolium
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235% activity compared to glycolate
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?
glyoxylate + reduced 2,6-dichlorophenolindophenol
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71.7% activity compared to D-lactate
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-
?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
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r
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
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?
glycolate + 2,6-dichlorophenolindophenol
glyoxylate + reduced 2,6-dichlorophenolindophenol
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-
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?
glyocylate + reduced acceptor
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the bacterial enzyme catalyzes phenazine methosulfate dependent reduction of 2,6-dichlorophenolindophenol or cytochrome c
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?
glycolate + acceptor
glyocylate + reduced acceptor
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in green algae the enzyme is part of glycolate metabolism
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?
glycolate + acceptor
glyocylate + reduced acceptor
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in green algae the enzyme is part of glycolate metabolism
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-
?
glycolate + acceptor
glyoxylate + reduced acceptor
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involved in the photorespiration pathway
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?
glycolate + acceptor
glyoxylate + reduced acceptor
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artificial acceptor 2,6-dichlorophenolindophenol is reduced via phenazine methosulfate, cytochrome c can replace 2,6-dichlorophenolindophenol
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?
glycolate + NAD+
glyoxylate + NADH
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the enzyme is involved in the bacterial 2-phosphoglycolate pathway, overview
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?
oxalate + reduced acceptor
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about 6% activity of glycolate
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?
glyoxylate + acceptor
oxalate + reduced acceptor
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2,6-dichlorophenolindophenol as acceptor, no reaction with O2 as acceptor
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?
?
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no activity with formate, malate, acetate, FAD, FMN, NAD+, NADP+, or cytochrome c
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?
additional information
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the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert
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?
additional information
?
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the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert
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?