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1.1.99.18: cellobiose dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about cellobiose dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.1.99.18

Reaction

cellobiose
+
acceptor
=
cellobiono-1,5-lactone
 +
reduced acceptor

Synonyms

cbdA, CBO, CBOR, Cdh, CDH IIA, CDH IIB, cdh-1, CDH1, Cdh2, CDHIIA, cellobiose (acceptor) 1-oxidoreductase, cellobiose dehydrogenase, cellobiose dehydrogenase IIA, cellobiose oxidase, cellobiose oxidoreductase, cellobiose [acceptor] 1-oxidoreductase, Cellobiose-quinone oxidoreductase, cellobiose:(acceptor) 1-oxidoreductase, cellobiose:quinone oxidoreductase, cellobiose:[acceptor] 1-oxidoreductase, DCHsr, dehydrogenase, cellobiose, EC 1.1.3.25, EC 1.1.5.1, MtCDH, oxidase, cellobiose, Thite_59724, TpCDH, TvCDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.18 cellobiose dehydrogenase (acceptor)

Specific Activity

Specific Activity on EC 1.1.99.18 - cellobiose dehydrogenase (acceptor)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.08
-
purification step culture filtration
0.17
-
purification step ultra filtration
0.28
-
isoform CDH2 from extracellular medium, at pH 6.0 and 50°C
1.19
-
isoform CDH1 from extracellular medium, at pH 6.0 and 50°C
1.3
purified mutant enzyme E279N, pH and temperature not specified in the publication
1.79
-
purification step POROS HP2
1.9
purified wild type enzyme, pH and temperature not specified in the publication
10
Thermothelomyces myriococcoides
-
-
10.7
Thermothelomyces myriococcoides
-
-
12.3
after 26.3fold purification, at pH 4.0 and 37°C
12.7
30°C, pH 4.5, substrates: lactose + cytochrome c
13.2
after 26.3fold purification, at pH 5.5 and 37°C
13.4
-
cytochrome c reduction at 60°C
14.4
after 73.41fold purification, at pH 4.5 and 30°C
14.7
pH 5.5, 30°C
1416
after 91.4fold purification, with lactose and cytochrome c as substrates, at pH 4.5 and 30°C
15.5
culture filtrate, with lactose and cytochrome c as substrates, at pH 4.5 and 30°C
16.3
-
purification step Mono Q
17.8
Thermothelomyces fergusii
-
after 7.3fold purification, at pH 5.0 and 37°C
1770
after 54.7fold purification, with lactose and ,6-dichlorophenolindophenol as substrates, at pH 4.5 and 30°C
2.18
Coniophora puteana (Schum ex Fr) Karsten
-
-
2.64
-
purification step HiLoad Superdex-200
2.7
after 14.8fold purification, at pH 4.5 and 37°C
3.1
Thermothelomyces myriococcoides
-
after 15.5fold purification, in 100 mM sodium acetate buffer, pH 5.0, at 30°C
32.3
culture filtrate, with lactose and 2,6-dichlorophenolindophenol as substrates, at pH 4.5 and 30°C
324
-
pH 5.0, 25°C, substrate: lactose
5.3
-
at pH 4.0 and 25°C
5.7
-
after 48.1fold purification, at pH 4.0 and 37°C
5.73
Thermothelomyces myriococcoides
-
-
62
-
assay detects both intact and truncated enzyme
66
-
electron acceptor cytochrome c
7.1
after 293fold purification, at pH 4.0 and 37°C
7.78
-
-
7.93
-
isoform CDH2 after 28.32fold purification, at pH 6.0 and 50°C
8
Thermothelomyces myriococcoides
after 3.6fold purification, at pH 6.0 and 30°C
8.89
-
isoform CDH1 after 7.47fold purification, at pH 6.0 and 50°C
8.9
Thermothelomyces fergusii
-
after 7.3fold purification, at pH 4.0 and 37°C
9
-
after 21.2fold purification, at pH 5.0 and 37°C
additional information