1.1.99.20: alkan-1-ol dehydrogenase (acceptor)

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For detailed information about alkan-1-ol dehydrogenase (acceptor), go to the full flat file.

Reaction

Synonyms

alkan-1-ol dehydrogenase, alkan-1-ol:acceptor oxidoreductase, dehydrogenase, polyethylene glycol, ethoxy chain nonylphenol dehydrogenase, NPEO-DH, PEG-DH, PEGDH, polyethylene glycol dehydrogenase

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.99 With unknown physiological acceptors

                1.1.99.20 alkan-1-ol dehydrogenase (acceptor)

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Results	in table
11	Activating Compound
3	AA Sequence
4	Application
1	CAS Registry Number
4	Cloned(Commentary)
27	Cofactor
46	Inhibitors
6	kcat/KM [mM/s]
65	KM Value [mM]
6	Localization
7	Metals/Ions
9	Molecular Weight [Da]
24	Organism
1	Pathway
4	pH Optimum
3	pH Range
7	pH Stability
5	Protein Variants
5	Purification (Commentary)
1	Reaction
3	Reaction Type
21	Reference
20	Specific Activity [micromol/min/mg]
179	Substrates and Products (Substrate)
6	Subunits
21	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
3	Temperature Range [°C]
6	Temperature Stability [°C]
6	Turnover Number [1/s]

Engineering

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Engineering on EC 1.1.99.20 - alkan-1-ol dehydrogenase (acceptor)

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

H465A

Ensifer sp. AS08

Q2HXX0

the mutant shows no significant change in affinity toward substrates, but the apparent kcat values decrease to approximately 1/20 of the apparent kcat value of the wild type enzyme

725161

H465A/N507A

Ensifer sp. AS08

Q2HXX0

inactive

725161

N507A

Ensifer sp. AS08

Q2HXX0

inactive

725161

N509A

Ensifer sp. AS08

Q2HXX0

the mutant shows the less kcat values toward NPEOav10 than toward PEG-1000

725161

N90A

Ensifer sp. AS08

Q2HXX0

the mutant shows the less kcat values toward NPEOav10 than toward PEG-1000

725161