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11
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.45 - 0.81
6-deoxy-D-glucose
9 - 13.5
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
8.8 - 12.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
1.1 - 18.33
cellopentaose
7.8 - 12.8
N-acetyl-D-glucosamine
0.45
6-deoxy-D-glucose
-
O2 used as electron acceptor
0.81
6-deoxy-D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
9
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
13.5
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
8.8
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
12.3
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.22
cellobiose
H70A mutant protein
0.58
cellobiose
C130A mutant protein
5.7
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
6.2
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
6.3
cellobiose
wild type enzyme, at 37°C and pH 8.0
7
cellobiose
wild type enzyme, at pH 8.0 and 37°C
7.4
cellobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
9
cellobiose
-
O2 used as electron acceptor
11.4
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
11.9
cellobiose
wild type protein
12
cellobiose
mutant enzyme A38V, at pH 8.0 and 37°C
13.7
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
13.7
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
13.72
cellobiose
pH 8.0, 37°C, recombinant mutant Y300A
16.33
cellobiose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
1.1
cellohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
6.6
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
7.2
cellohexaose
wild type enzyme, at pH 8.0 and 37°C
10
cellohexaose
-
O2 used as electron acceptor
13.2
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
16.5
cellohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
1.1
cellopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor
7.6
cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
8.3
cellopentaose
wild type enzyme, at pH 8.0 and 37°C
9
cellopentaose
-
O2 used as electron acceptor
12.7
cellopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
18.33
cellopentaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
1.4
cellotetraose
-
2,6-dichlorophenol indophenol used as electron acceptor
4.17
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
7.8
cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
9.9
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
11
cellotetraose
-
O2 used as electron acceptor
12.8
cellotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
1.5
cellotriose
-
2,6-dichlorophenol indophenol used as electron acceptor
5.3
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
6
cellotriose
wild type enzyme, at 37°C and pH 8.0
6.9
cellotriose
wild type enzyme, at pH 8.0 and 37°C
7.3
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
7.5
cellotriose
-
O2 used as electron acceptor
10
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
11.17
cellotriose
pH 8.0, 37°C, recombinant mutant Y300A
11.2
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
11.2
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
13.7
cellotriose
mutant enzyme A38V, at pH 8.0 and 37°C
18.67
cellotriose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.25
D-galactose
-
2,6-dichlorophenol indophenol used as electron acceptor
1.5
D-galactose
-
O2 used as electron acceptor
6.6
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
7.2
D-galactose
wild type enzyme, at 37°C and pH 8.0
11.8
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
13.3
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
0.46
D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
2.1
D-glucose
-
O2 used as electron acceptor
5.6
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
6
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
6.3
D-glucose
wild type enzyme, at pH 8.0 and 37°C
7.5
D-glucose
wild type enzyme, at 37°C and pH 8.0
10.4
D-glucose
mutant enzyme A38V, at pH 8.0 and 37°C
10.8
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
13.2
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
13.2
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37°C
13.22
D-glucose
pH 8.0, 37°C, recombinant mutant Y300A
15.68
D-glucose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.58
D-xylose
-
2,6-dichlorophenol indophenol used as electron acceptor
1.7
D-xylose
-
O2 used as electron acceptor
4.6
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
5.3
D-xylose
wild type enzyme, at 37°C and pH 8.0
5.5
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
7.4
D-xylose
wild type enzyme, at pH 8.0 and 37°C
11.3
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
11.3
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37°C
11.33
D-xylose
pH 8.0, 37°C, recombinant mutant Y300A
11.7
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
12.4
D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
16.25
D-xylose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.73
L-arabinose
-
O2 used as electron acceptor
1.1
L-arabinose
-
2,6-dichlorophenol indophenol used as electron acceptor
4.4
lactose
-
2,6-dichlorophenol indophenol used as electron acceptor
6.3
lactose
-
O2 used as electron acceptor
0.35
maltohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.57
maltohexaose
-
O2 used as electron acceptor
0.9
maltopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor
1.8
maltopentaose
-
O2 used as electron acceptor
0.76
maltose
-
2,6-dichlorophenol indophenol used as electron acceptor
2
maltose
-
O2 used as electron acceptor
5.4
maltose
mutant enzyme W351F, at 37°C and pH 8.0
6
maltose
wild type enzyme, at 37°C and pH 8.0
10.4
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
10.4
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
2.3
maltotetraose
-
O2 used as electron acceptor
2.6
maltotetraose
-
2,6-dichlorophenol indophenol used as electron acceptor
2.2
maltotriose
-
2,6-dichlorophenol indophenol used as electron acceptor
2.3
maltotriose
-
O2 used as electron acceptor
7.8
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
8.1
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
11.3
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
12.8
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
3
xylobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
3.3
xylobiose
-
O2 used as electron acceptor
7.5
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
8
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
8.7
xylobiose
wild type enzyme, at pH 8.0 and 37°C
8.8
xylobiose
wild type enzyme, at 37°C and pH 8.0
12
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
13.28
xylobiose
pH 8.0, 37°C, recombinant mutant Y300A
13.3
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
13.3
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
14.8
xylobiose
mutant enzyme A38V, at pH 8.0 and 37°C
19.72
xylobiose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
7.8
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
8.6
xylohexaose
wild type enzyme, at pH 8.0 and 37°C
15.2
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
17.43
xylohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
8.8
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
10.4
xylopentaose
wild type enzyme, at pH 8.0 and 37°C
15.2
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
26.17
xylopentaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
8.1
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
9.7
xylotetraose
wild type enzyme, at pH 8.0 and 37°C
16
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
7.9
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
8.3
xylotriose
wild type enzyme, at 37°C and pH 8.0
8.7
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
10.3
xylotriose
wild type enzyme, at pH 8.0 and 37°C
12
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
13.87
xylotriose
pH 8.0, 37°C, recombinant mutant Y300A
13.9
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
13.9
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
14.5
xylotriose
mutant enzyme A38V, at pH 8.0 and 37°C
19.67
xylotriose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
1.1.99.B3 glucooligosaccharide oxidase
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