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1.10.3.1: catechol oxidase

This is an abbreviated version!
For detailed information about catechol oxidase, go to the full flat file.

Word Map on EC 1.10.3.1

Reaction

2 catechol +

O2
= 2 1,2-benzoquinone + 2 H2O

Synonyms

1,2-benzene: oxygen oxidoreductase, 1,2-benzenediol:oxygen oxidoreductase, AoCO4, catalase-phenol oxidase, catechol oxidase, catecholase, catecholoxidase, CATPO, CO, copper-S100B, cresolase, dihydroxy-L-phenylalanine:oxygen oxidoreductase, Diphenol oxidase, diphenolase, dopa oxidase, germin-like protein, GLP, hemocyanin, ibCO, LsPPO, mettyrosinase, monophenol, o-diphenol: oxygen oxidoreductase, monophenol, o-diphenol:oxygen oxidoreductase, More, MppO, o-diphenol oxidoreductase, o-diphenol: dioxygen oxidoreductase, dehydrogenating, o-diphenol:oxygen oxidoreductase, o-diphenolase, o-diphenoloxidase, oxytyrosinase, phenol oxidase, phenolase, phenoloxidase, polyphenol oxidase, polyphenoloxidase, PPO, PPO 1, PPO 2, PPO II, PPO-6, pyrocatechol oxidase, TYR3, tyrosinase

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.1 catechol oxidase

Purification

Purification on EC 1.10.3.1 - catechol oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
99% purity
-
ammonium sulfate precipitation and DEAE-cellulose column chromatography
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ammonium sulfate precipitation, and phenyl Sepharose column chromatography
-
ammonium sulfate precipitation, column chromatography, gel filtration
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Sephacryl S-100 gel filtration
-
ammonium sulfate precipitation, Superose-R 12 column chromatography, and Sephadex G-75 gel filtration
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex, hydroxyapatite, electrofocusing
-
ammonium sulfate, DEAE-Sephacel, Phenyl-agarose, Sephadex G-100
-
ammonium sulfate, DEAE-Sephadex A-50, Sephadex G-75
-
ammonium sulfate, DEAE-toyopearl, butyl-toyopearl, Super Q toyopearl, hydroxyapatite, toyopearl HW 55
-
AoCO4 is purified with a two-step purification procedure, consisting of cation and anion exchange chromatography
-
DEAE-Sepharose column chromatography and Sephadex G-75 gel filtration
-
DEAE-toyopearl, copper-affinity chromatography, Sephadex 200, PAGE
-
hexadecyltrimethylammonium bromide extraction, ammonium sulfate, Q-Sepharose, Phenyl-Sepharose, hydroxylapatite
ion exchange chromatography
-
isoenzymes A, B, C and D, acetone precipitate, DEAE-cellulose
-
isoenzymes Ia, Ib and II ammonium sulfate, DEAE-cellulose, Sephacryl S-200, hydroxyapatite, Mono Q
-
native enzyme 11.5fold by (NH4)2SO4 precipitation, dialysis, and L-tyrosine-4-aminobenzoic acid affinity chromatography
-
native enzyme 17.8fold by (NH4)2SO4 precipitation, dialysis, and ion exchange chromatography
-
native enzyme 170fold from fruits by acetone and ATPS precipitation
-
native enzyme 54.41fold by temperature-induced phase partitioning technique, dialysis, and ion exchange chromatography
-
native enzyme 75.5fold to homogeneity from apples purification in presence of AEBSF and aprotinin by ammonium sulfate fractionation, dialysis, gel filtration, and hydrophobic interaction chromatography, followed by anion exchange and hydroxyapatite chromatography
-
native enzyme 78fold to homogeneity
-
native enzyme by anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration
-
native enzyme from corn tassel 10fold by ammonium sulfate fractionation and gel filtration
-
native enzyme from leaves 2.54fold by ammonium sulfate fractionation and gel filtration
-
native enzyme from seeds
-
native enzyme partially, solubilization by Triton X-100 in presence of polyvinyl polypyrrolidone, 32.fold by ammonium sulfate fractionation and dialysis
-
native enzyme, 25.8fold from stem and 43.3 from leaves, by (NH4)2SO4 precipitation, dialysis, and gel filtration
Ferula sp.
-
native enzyme, partially from stem, root, and leaves, by (NH4)2SO4 precipitation and dialysis
-
native soluble, active enzyme form partially 3.3fold by ammonium sulfate fractionation, particulate, latent active enzyme form 40fold to homogeneity by phase partitioning in Triton X-114 followed by anion exchange and hydrophobic interaction chromatography, and gel filtration
-
native tyrosinase from hemolymph to homogeneity by anion exchange and hydrophobic interaction chromatography
-
Ni-NTA column chromatography
-
nickel chelate column chromatography
-
optimization of enzyme extraction from roots, best using 0.2 M phosphate buffer at pH 7.0 with 5% insoluble polyvinylpyrrolidone and 0.25% Triton X-100, overview
-
partially purified in a sequential two-phase system based on Triton X-114 and PEG-8000/phosphate, followed by ammonium sulfate fractionation
-
phenol oxidases A and B, DEAE-Sepharose, partially purified
-
purified by gel filtration
-
recombinant secreted enzyme 4.9fold from culture supernatant by cation exchange chromatography and gel filtration to homogeneity
-
Sephacryl S-300 gel filtration
-
using (NH4)2SO4 precipitation and ion-exchange chromatography
-
using a Sephadex G-200 column balanced with 0.1 M sodium phosphate buffer (pH 6.5)
-
using a Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column
-