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1,4-diethylenedioxide
-
complete inhibition at 50% or above
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
-
shows substrate inhibition at high concentrations above 6 mM
2,2-bipyridyl
-
1 mM, 61% residual activity
2,2-dipyridyl
1 mM, 13.4% loss of activity. 10 mM, 24.8% loss of activity
2,3-Dimercaptopropanol
-
-
2,4-dithio-6-pyrimidine
-
1 mM, 31% residual activity
2,6-dimethoxyphenol
substrate inhibition
2-mercaptobenzothiazole
Thermochaetoides thermophila
-
-
3,4,5-hydroxybenzoic acid propyl ester
-
-
4,5-methyl-o-phenylenediamine
-
50% inhibition at 5 mM
4-coumaric acid
32.5% residual activity at 1 mM
4-hydroxyphenylacetic acid
-
effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
4-hydroxyphenylpropionic acid
-
effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
4-nitrophenol
-
effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
5,5 dithiobis(2-nitrobenzoate)
Acetylacetone
-
1 mM, 27% inhibition
Al2+
-
3 mM, 23% inhibition
-
AlCl3
-
10 mM, 40% inhibition
ascorbic acid
Thermochaetoides thermophila
-
-
BaCl2
5 mM, 10% inhibition
bathocuproinedisulfonic acid
-
1 mM, 39% residual activity
Benzene
-
1 h, 20% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v)
bipyridyl
-
90% inhibition
butanol
-
25%, 42% inhibition
Cationic detergents
-
-
-
CdCl2
-
1 mM, 24.6% inhibition
Cetylpyridinium bromide
-
-
cetyltrimethyl ammonium bromide
50% inhibition
cetyltrimethylammonium bromide
-
-
chitosan
-
conjugated to the enzyme, with 81-93% laccase being conjugated, a moderate activity loss of 16-28% occurs in conjugation solution, a second severe activity loss of 63-78% occurs during a cycle of phase change consisting of precipitation, centrifugation and re-dissolution of the enzymechitosan conjugates, the chitosan molecular size has little effect, overview
Co3+
20 mM, weak inhibitory effect
CoSO4
-
10 mM, 9% inhibition
Cr2+
-
10 mM, 5% inhibition
Dichloromethane
-
50% (v/v), 80% inhibition
diethyldithiocarbamic acid
-
-
Diethylenetriaminepentaacetic acid
-
10 mM, 76% loss of activity
dimethyldithiocarbamate trihydrate
-
1 mM, 13% residual activity
DL-dithiothreitol
0.01 mM, 9.5% loss of activity. 0.1 mM, complete loss of activity
FeCl2
MK290990.1
1 mM, 30 min, 84.47% residual activity
H2O2
significantly inhibits at above 4.85 mmol/l
hydroxylammonium chloride
-
-
isoascorbic acid
Thermochaetoides thermophila
-
-
KCN
competitive, complete inhibition
KI
-
5 mM, 25% inhibition
MgCl2
MK290990.1
1 mM, 30 min, 89.34% residual activity
MgSO4
-
10 mM, 6% inhibition
MnSO4
-
10 mM, 8% inhibition
Mo2+
-
100 mM, 82% inhibition
N,N-Dimethylformamide
-
1 h, 10% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v)
N-hydroxylglycine
-
0.1 mM, 87% inhibition
Na2SO4
-
100 mM, 7% inhibition
NaNO3
-
100 mM, 50% inhibition
Neocuprein
-
50% inhibition at 5 mM
NiCl2
MK290990.1
1 mM, 30 min, 80.65% residual activity
NiSO4
5 mM, 4% inhibition
oxalate
-
1 mM, 35% inhibition
p-coumarate
-
0.1 mM, complete inhibition
p-nitrophenol
-
50% inhibition at 5 mM
Phenanthroline
-
95% inhibition
Phenylthiourea
-
50% inhibition at 3 mM
potassium xanthogenate
Thermochaetoides thermophila
-
-
Propanol
-
25%, 17% inhibition
Salicylhydroxamic acid
-
0.08 mM, 85% inhibition
Semicarbazide
-
25% decrease in enzyme activity
Sn2+
4.6% residual activity at 1 mM
SnCl2
-
1 mM, 69% residual activity
Sodium bisulfite
Thermochaetoides thermophila
-
-
sodium bromide
-
50% inhibition at 195 mM
sodium chloride
-
50% inhibition at 52 mM
Sodium fluoride
-
50% inhibition at 0.08 mM
Sodium metabisulfite
Thermochaetoides thermophila
-
-
Sodium thioglycolate
-
45.3% residual activity at 10 mM
thiamine
-
50% inhibition at 0.95 mM
thiolglycolic acid
-
completely inhibited by 60 mM
Toluene
-
20% (v/v), 18% loss of activity
Triton X
-
2%, 61% inhibition
-
Triton X-100
Sporothrix carnis
-
1 mM, 35% inhibition
Tween 80
-
2%, 51% inhibition
Tween-80
1%, more than 50% loss of activity
1,10-phenanthroline
1 mM, 14.7% loss of activity. 10 mM, 89.1% loss of activity
1,10-phenanthroline
-
10 mM, complete loss of activity
1,10-phenanthroline
-
10 mM, complete loss of activity
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
200 mM, 80% inhibition
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
-
99.5% inhibition
1-Phenyl-2-thiourea
-
-
1-Phenyl-2-thiourea
Thermochaetoides thermophila
-
-
2-mercaptoethanol
-
0.05 mM, 29% loss of activity
2-mercaptoethanol
0.1 mM, 65.5% inhibition
2-mercaptoethanol
-
1 mM, complete inactivation
2-mercaptoethanol
competitive, complete inhibition
2-mercaptoethanol
-
1 mM, 68% inhibition
2-mercaptoethanol
-
1 mM, 97% inhibition
2-mercaptoethanol
-
complete inhibition of laccase activity at 0.1% concentration
2-mercaptoethanol
-
complete inhibition at 0.1 mM
2-mercaptoethanol
-
strong
2-mercaptoethanol
-
12.5 mM, complete inhibition
2-mercaptoethanol
Sporothrix carnis
-
1 mM, 23% inhibition
2-mercaptoethanol
Thermochaetoides thermophila
-
-
2-mercaptoethanol
-
93% inhibition at 1 mM
2-mercaptoethanol
-
5 mM, 15% loss of activity
4-chloromercuribenzoate
-
-
4-chloromercuribenzoate
-
-
4-chloromercuribenzoate
-
-
5,5 dithiobis(2-nitrobenzoate)
-
-
5,5 dithiobis(2-nitrobenzoate)
-
-
5,5 dithiobis(2-nitrobenzoate)
-
-
8-hydroxyquinoline
-
-
8-hydroxyquinoline
-
70% inhibition at 1 mM
acetone
-
mutant enzyme V159E/N398D
acetone
-
50% (v/v), 90% inhibition
acetone
-
complete inhibition at 50% or above
acetone
31% (v/v) 50% inhibition
acetone
-
25%, 20% inhibition
acetone
-
20% (v/v), 42% loss of activity
acetonitrile
-
completely inhibits enzyme activity
acetonitrile
-
complete inhibition at 50% or above
acetonitrile
4.5% (v/v) 50% inhibition
Ag+
-
complete inhibition at 20 mM
Ag+
-
8.13% inhibition at 10 mM
Al3+
-
10 mM, 3% inhibition
Al3+
activating at 1 mM, inhibitory at 10 mM
Al3+
strongly inhibits at 10 mM
Al3+
-
5 mM, 44% inhibition
Al3+
-
1 mM, 43% inhibition
Al3+
-
100 mM, 92% inhibition
Al3+
-
10 mM, 43% loss of activity
Al3+
-
14% inhibition at 5 mM
Al3+
-
11.6% inhibition at 1 mM
Al3+
-
5 mM, 60% loss of activity
azide
-
-
azide
-
100% inhibition at 0.1 mM
azide
-
85% inhibition at 1 mM
azide
Phlebia fascicularia
-
complete inhibition
azide
-
100 inhibition at 0.1 mM
azide
-
55-70% inhibition at 0.5 mM
azide
Thermochaetoides thermophila
-
-
azide
-
complete inhibition at 0.02 mM
azide
-
0.1 mM, 100% inhibition
Ba2+
-
10 mM, 16% inhibition
Ba2+
partially inhibits laccase activity
Ba2+
-
1 mM, 11% loss of activity
Ba2+
activating at 1 mM, inhibitory at 10 mM
Ba2+
-
5 mM, 27% inhibition
Ba2+
-
10 mM, 59% inhibition
Ba2+
-
1 mM, 52% inhibition
Ba2+
Sporothrix carnis
-
1 mM, 39% inhibition
Ba2+
-
10 mM, 26% loss of activity
Ba2+
-
10% inhibition at 1 mM
Ca2+
-
10 mM, 6% inhibition
Ca2+
-
1.25 mM, 8% inhibition. 10 mM, 17% inhibition
Ca2+
activating at 1 mM, inhibitory at 10 mM
Ca2+
-
1% residual activity at 10 mM; 86.6% residual activity at 10 mM
Ca2+
-
5 mM, 17% inhibition
Ca2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Ca2+
-
significantly inhibition
Ca2+
-
10 mM, 80% inhibition
Ca2+
-
100 mM, 43% inhibition
Ca2+
89.8% residual activity at 1 mM
Ca2+
-
10 mM, 35% loss of activity
Ca2+
Sporothrix carnis
-
1 mM, 37% inhibition
Ca2+
-
200 mM, 66.72% residual activity
Ca2+
-
30 mM, 85% loss of activity
CaCl2
-
10 mM, 31% inhibition
CaCl2
MK290990.1
1 mM, 30 min, 90.15% residual activity
Cd2+
-
10 mM, 81% inhibition
Cd2+
-
1.25 mM, 6% inhibition. 10 mM, 20% inhibition
Cd2+
-
1 mM, 28% of inhibition
Cd2+
-
96.2% residual activity at 10 mM
Cd2+
-
71.1% residual activity at 20 mM
Cd2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Cd2+
-
21% inhibition at 1 mM
Cd2+
-
significantly inhibition
Cd2+
-
100 mM, 92% inhibition
Cd2+
88.0% residual activity at 1 mM
Cd2+
-
40% inhibition at 20 mM, almost complete inhibition at 80 mM
Cd2+
-
5 mM, 78% loss of activity
chloroform
-
1 h, 20% loss of activity by 20% (v/v), 65% loss of activity by 50% (v/v)
chloroform
-
25%, 25% inhibition
Citric acid
-
CN-
-
-
CN-
-
100% inhibition at 1 mM
CN-
-
40-65% inhibition at 0.5 mM
CN-
Thermochaetoides thermophila
-
-
Co2+
-
1 mM, 79%% inhibition
Co2+
-
1.25 mM, 3% inhibition. 10 mM, 17% inhibition
Co2+
-
1 mM, 51% of inhibition
Co2+
-
1 mM, complete inhibition
Co2+
-
61.9% residual activity at 20 mM
Co2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Co2+
-
29% inhibition at 1 mM
Co2+
-
significantly inhibition
Co2+
-
1 mM, 63% inhibition
Co2+
-
10 mM, 55% inhibition
Co2+
-
100 mM, 51% inhibition
Co2+
75.3% residual activity at 01 mM
Co2+
-
28% inhibition at 5 mM
Co2+
-
10 mM, 99% loss of activity
Co2+
-
5 mM, 67% loss of activity
CoCl2
5 mM, 19% inhibition
CoCl2
-
1 mM, 20.8% inhibition
Cr3+
activating at 1 mM, inhibitory at 10 mM
Cr3+
-
46.2% residual activity at 20 mM
Cr3+
-
23% inhibition at 5 mM
Cr3+
-
inhibits 13.8% at 1 mM
Cu2+
-
10 mM, 4% inhibition
Cu2+
-
1.25 mM, 9% inhibition. 10 mM, 19% inhibition
Cu2+
-
10 mM, 25% inhibition
Cu2+
-
68.9% residual activity at 20 mM
Cu2+
-
10 mM, 61% inhibition
Cu2+
-
3 mM, 16% inhibition
Cu2+
Sporothrix carnis
-
1 mM, 48% inhibition
Cu2+
-
200 mM, 65.15% residual activity
Cu2+
-
19% inhibition at 5 mM
Cu2+
-
10 mM, 23% loss of activity
Cu2+
-
40% inhibition at 20 mM, almost complete inhibition at 80 mM
CuSO4
-
10 mM, 19% inhibition
CuSO4
MK290990.1
1 mM, 30 min, 90.81% residual activity
cyanide
Phlebia fascicularia
-
complete inhibition
cysteine
-
-
cysteine
-
5 mM, 92% loss of activity
cysteine
Thermochaetoides thermophila
-
-
cysteine
-
0.1 mM, 100% inhibition
cysteine-HCl
-
complete inhibition at 0.1 mM
cysteine-HCl
Opuntia vulgaris
-
complete inhibition at 0.1 mM
desferrioxamine mesylate
-
-
desferrioxamine mesylate
-
-
desferrioxamine mesylate
-
-
diethyldicarbonate
-
inactivation in simple linear pseudo-first order kinetics, recovery by 0.5 M hydroxylamine
diethyldicarbonate
-
inactivation in simple linear pseudo-first order kinetics, recovery by 0.5 M hydroxylamine
diethyldicarbonate
-
inactivation in simple linear pseudo-first order kinetics, recovery by 0.5 M hydroxylamine
diethyldithiocarbamate
-
50% inhibition at 1.2 mM
diethyldithiocarbamate
-
-
diethyldithiocarbamate
-
-
diethyldithiocarbamate
-
15% inhibition at 1 mM
diethyldithiocarbamate
-
35% inhibition at 1 mM
diethyldithiocarbamate
-
50% inhibition at 0.02 mM
diethyldithiocarbamate
-
-
dimethyl sulfoxide
-
50% (v/v), 85% inhibition
dimethyl sulfoxide
-
1 h, 10% loss of activity by 20% (v/v), 40% loss of activity by 50% (v/v)
dithiothreitol
-
dithiothreitol
-
1 mM, complete inactivation
dithiothreitol
0.1 mM, complete inhibition
dithiothreitol
-
5 mM, complete inactivation. 1 mM, 80% loss of activity
dithiothreitol
-
activates laccase at lower concentration (0.1 mM) while inhibits the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition of activity at 10 mM
dithiothreitol
0.1 mM, activity is reduced to 5.9%
dithiothreitol
0.1 mM, complete inactivation; 0.1 mM, complete inactivation
dithiothreitol
-
0.1 mM, complete inhibition
dithiothreitol
-
0.1 mM, complete inhibition
dithiothreitol
-
complete inhibition at 0.1 mM
dithiothreitol
-
complete inhibition at 2 mM
dithiothreitol
-
1 mM, 61% inhibition
dithiothreitol
-
5 mM, 81% inhibition
dithiothreitol
-
1 mM, 100% inhibition
dithiothreitol
-
0.1 mM, 98% inhibition
dithiothreitol
completely inhibited by 1.0 mM dithiothreitol
dithiothreitol
-
1 mM, 10% loss of activity
dithiothreitol
-
strong inhibition at 1 mM
dithiothreitol
-
5 mM, 65% loss of activity
dithiothreitol
-
0.1 mM, 100% inhibition
DMSO
-
mutant enzyme V159E/N398D
DMSO
10%, 50% loss of the original activity
DMSO
-
10 mM, 34% inhibition
DMSO
40% (v/v) 50% inhibition
DTT
0.1 mM, complete inhibition
DTT
complete inhibition at 5 mM
DTT
1 mM, complete inhibition
DTT
-
complete inhibition at 0.1 mM
DTT
-
43% inhibition at 10 mM
DTT
-
complete inhibition at over 10 mM with substrate 3,6-dimethoxyphenol and at over 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
DTT
-
complete inhibition at 1 mM
EDTA
-
-
EDTA
5 mM or 20 mM, partially inhibits activity
EDTA
-
mutant enzyme V159E/N398D
EDTA
-
1 mM, 31% inhibition
EDTA
-
50% inhibition at about 300 mM
EDTA
-
0.5 mM, 29% loss of activity
EDTA
5 mM, 51.5% inhibition
EDTA
-
28% inhibition at 40 mM
EDTA
-
1 mM, 15.6% loss of activity
EDTA
-
10 mM, 77% loss of activity
EDTA
-
5 mM, 84-87% inhibition (depending on purification method)
EDTA
-
10 mM, 10% inhibition
EDTA
50 mM, 70% loss of activity, laccase 3; 50 mM, 80% loss of activity, laccase 4
EDTA
-
10 mM, 6% inhibition
EDTA
1 mM, 67% inhibition
EDTA
-
1 mM, 90% residual activity
EDTA
-
complete inhibition at 1 mM
EDTA
-
1 mM, 20% inhibition
EDTA
-
6.8% residual activity at 1 mM, complete inhibition at 10 mM
EDTA
-
20.8% residual activity at 20 mM
EDTA
-
1 mM, 21% inhibition
EDTA
-
87% inhibition at 1 mM, complete inhibition at 2 mM
EDTA
-
1 mM, 95% inhibition
EDTA
-
10 mM, 6% inhibition
EDTA
Opuntia vulgaris
-
complete inhibition at 1 mM
EDTA
-
10 mM, 36% inhibition
EDTA
-
0.1 mM, complete inhibition
EDTA
Phlebia fascicularia
-
partiall inhibition
EDTA
-
60% inhibition at 100 mM
EDTA
50% residual activity at 10 mM
EDTA
-
completely inhibited by 200 mM
EDTA
-
16% inhibition at 10 mM
EDTA
Sporothrix carnis
-
1 mM, 29% inhibition
EDTA
-
5 mM, complete inhibition
EDTA
-
1 mM, 62% loss of activity
EDTA
-
1 mM, 67% residual activity
EDTA
MK290990.1
100 mM, 93.1% residual activity
EDTA
Thermochaetoides thermophila
-
5 mM, 67% inhibition. 10 mM, 95% inhibition
EDTA
the reaction in the presence of 0.1 mM CuSO4 is completely inhibited by the addition of 1 mM EDTA
EDTA
-
10 mM, 35% inhibition
EDTA
un competitive inhibition
EDTA
-
1 mM, 74.2% inhibition
EDTA
-
45% inhibition at 10 mM, 10% at 1 mM
EDTA
-
5 mM, 70% loss of activity
EDTA
-
1 mM, 20% inhibition
EGTA
-
-
EGTA
-
22% inhibition at 10 mM
ethanol
-
mutant enzyme V159E/N398D
ethanol
-
50% inhibition at 36%
ethanol
-
50% (v/v), 80% inhibition
ethanol
-
1 h, 20% loss of activity by 20% (v/v), 45% loss of activity by 50% (v/v)
ethanol
52% (v/v) 50% inhibition
ethanol
-
25%, 28% inhibition
ethanol
-
20% (v/v), 15% loss of activity
F-
Polyporus versicolor
-
-
F-
-
complete inhibition at 1 equivalent F- per type 2 Cu2+
Fe2+
partially inhibits laccase activity
Fe2+
-
5 mM, 59% loss of activity
Fe2+
10 mM, 94% loss of activity
Fe2+
-
1.25 mM, 31% inhibition. 10 mM, 61% inhibition
Fe2+
activating at 1 mM, inhibitory at 10 mM
Fe2+
-
complete inhibition at 10 mM
Fe2+
-
8.47% residual activity at 10 mM
Fe2+
-
4.0% residual activity at 20 mM
Fe2+
-
1 mM, reduces LacG activity to 55%
Fe2+
-
significantly inhibition
Fe2+
-
1 mM, complete inhibition
Fe2+
FJ560721
inhibits laccase activity completely at 10 mM
Fe2+
Opuntia vulgaris
-
complete inhibition at 10 mM
Fe2+
-
complete inhibition at 1 mM
Fe2+
-
50% inhibition at 50 mM
Fe2+
-
10 mM, 72.4% loss of activity
Fe2+
-
complete inhibition at 10 mM
Fe2+
-
10 mM, about 75% inhibition
Fe2+
-
200 mM, no residual activity
Fe2+
-
95% inhibition at 5 mM
Fe2+
-
10 mM, 97% loss of activity
Fe2+
-
5 mM, 60% loss of activity
Fe3+
-
10 mM, 29% inhibition
Fe3+
partially inhibits laccase activity
Fe3+
-
1.25 mM, 46% inhibition. 10 mM, 93% inhibition
Fe3+
activating at 1 mM, inhibitory at 10 mM
Fe3+
strongly inhibits at 10 mM
Fe3+
-
complete inhibition at 20 mM
Fe3+
-
complete inhibition at 1 mM
Fe3+
-
50% inhibition at 10 mM
FeCl3
5 mM, 14% loss of activity
FeCl3
-
5 mM, complete inhibition
Ferrous sulfate
-
-
Ferrous sulfate
Thermochaetoides thermophila
-
-
FeSO4
5 mM, complete loss of activity
glutathione
-
1 mM, complete inhibition
Hg2+
-
10 mM, 79% inhibition
Hg2+
-
1 mM, 4% inhibition
Hg2+
-
1.0 mM, 48% loss of activity
Hg2+
-
concentration-dependent inhibition up to 1 mM
Hg2+
-
1 mM, 93% of inhibition
Hg2+
-
7.4% residual activity at 5 mM
Hg2+
-
14% inhibition at 1 mM
Hg2+
-
significantly inhibition
Hg2+
-
1 mM, 50% inhibition
Hg2+
-
strong inhibition at 1 mM
Hg2+
-
1 mM, 44% inhibition. 3 mM, 95% inhibition
Hg2+
Sporothrix carnis
-
1 mM, 46% inhibition
Hg2+
Thermochaetoides thermophila
-
-
Hg2+
-
200 mM, no residual activity
Hg2+
-
10 mM, 80% loss of activity
Hg2+
-
inhibits 17.2% at 1 mM
Hg2+
-
5 mM, 90% loss of activity
HgCl2
competitive, complete inhibition
HgCl2
-
1 mM, 67% residual activity
HgCl2
-
1 mM, 33.3% inhibition
HgCl2
-
46% inhibition at 51 mM
hydroxylamine
-
competitive
hydroxylamine
-
1 mM, 62% residual activity
Isopropanol
-
1 h, 40% loss of activity by 20% (v/v), 65% loss of activity by 50% (v/v)
Isopropanol
60% (v/v) 50% inhibition
Isopropanol
-
20% (v/v), 30% loss of activity
K+
-
10 mM, 26% inhibition
K+
-
10 mM, 4% inhibition
K+
activating at 1 mM, inhibitory at 10 mM
K+
-
1 mM, 4% inhibition, 10 mM, 15% inhibition
K+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
K+
65.1% residual activity at 10 mM
K+
-
10 mM, 7% loss of activity
KCl
-
10 mM, 21% inhibition
KCl
MK290990.1
1 mM, 30 min, 80.25% residual activity
kojic acid
12.5 mM, about 50% loss of activity
kojic acid
5 mM, complete inhibition
kojic acid
-
1 mM, 57% residual activity
kojic acid
-
47% inhibition at 1 mM
kojic acid
27.7% residual activity at 1 mM
kojic acid
-
3 mM, 48% inhibition
L-cysteine
-
1 mM, 95% inhibition
L-cysteine
-
50% inhibition at 0.52 mM
L-cysteine
-
0.05 mM, 44% loss of activity
L-cysteine
0.5 mM, 67% inhibition
L-cysteine
-
1 mM, complete inactivation
L-cysteine
0.1 mM, complete inhibition
L-cysteine
-
1 mM, reduced 87% of its original activity
L-cysteine
-
0.1 mM, complete inhibition
L-cysteine
0.1 mM, activity is reduced to 5.5%
L-cysteine
0.1 mM, complete inhibition
L-cysteine
-
0.1 mM, complete inhibition
L-cysteine
-
1 mM, 100% inhibition
L-cysteine
0.1 mM, 17% loss of activity. 1 mM, complete loss of activity
L-cysteine
-
89% inhibition at 0.1 mM, complete inhibition at 1 mM
L-cysteine
-
1 mM, 100% inhibition
L-cysteine
-
0.1 mM, 95% inhibition
L-cysteine
-
5 mM, complete inhibition
L-cysteine
completely inhibited by 1.0 mM L-cysteine
L-cysteine
-
50 mM, 15% inhibition
L-cysteine
-
44% inhibition at 10 mM
L-cysteine
-
0.5 mM, 25% inhibition
L-cysteine
-
complete inhibition at 10 mM
L-cysteine
-
1 mM, 65.05% inhibition
L-cysteine
-
strong inhibition at 1 mM
L-cysteine
-
98% inhibition at 1 mM
L-cysteine
-
90% inhibition at 10 mM, 70% at 1 mM
L-cysteine
-
5 mM, 20% loss of activity
Li+
-
5 mM, 22% loss of activity
Li+
10 mM, 51% loss of activity
Li+
activating at 1 mM, inhibitory at 10 mM
Li+
-
1 mM, 19% inhibition
methanol
-
50% inhibition at 37%
methanol
-
50% (v/v), 70% inhibition
methanol
-
completely inhibits enzyme activity
methanol
-
1 h, 20% loss of activity by 20% (v/v), 60% loss of activity by 50% (v/v)
methanol
-
complete inhibition at 50% or above
methanol
52% (v/v) 50% inhibition
methanol
-
25%, 17% inhibition
methanol
-
20% (v/v), 20% loss of activity
Mg2+
-
1 mM, 88% inhibition
Mg2+
-
5 mM, 23% loss of activity
Mg2+
-
10 mM, 8% inhibition
Mg2+
activating at 1 mM, inhibitory at 10 mM
Mg2+
-
84.4% residual activity at 10 mM
Mg2+
-
5 mM, 34% inhibition
Mg2+
-
significantly inhibition
Mg2+
-
10 mM, 66% inhibition
Mg2+
-
100 mM, 33% inhibition
Mg2+
68.8% residual activity at 10 mM
Mg2+
-
10 mM, 38% loss of activity
Mg2+
-
3 mM, 11% inhibition
Mg2+
Sporothrix carnis
-
1 mM, 45% inhibition
Mg2+
-
200 mM, 81.32% residual activity
Mg2+
-
6% inhibition at 5 mM
Mg2+
-
10 mM, 23% loss of activity
Mg2+
-
2.5 mM 9% inhibition
Mn2+
-
5 mM, 21% loss of activity
Mn2+
activating at 1 mM, inhibitory at 10 mM
Mn2+
strongly inhibits at 10 mM
Mn2+
-
1 mM, 91% of inhibition
Mn2+
-
2% residual activity at 10 mM
Mn2+
-
5 mM, 20% inhibition
Mn2+
-
49.1% residual activity at 20 mM
Mn2+
-
inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM
Mn2+
-
22% inhibition at 1 mM
Mn2+
-
significantly inhibition
Mn2+
-
10 mM, 72% inhibition
Mn2+
-
1 mM, 51% inhibition
Mn2+
-
100 mM, 44% inhibition
Mn2+
82.3% residual activity at 10 mM
Mn2+
-
10 mM, 38% loss of activity
Mn2+
Sporothrix carnis
-
1 mM, 45% inhibition
Mn2+
-
10 mM, about 30% inhibition
Mn2+
-
50 mM, 100% loss of activity
MnCl2
5 mM, 90% loss of activity
MnCl2
-
1 mM, 83% residual activity
Na+
-
10 mM, 11% inhibition
Na+
-
exhibits activation of laccase up to 1 mM beyond which it exhibits inhibition of laccase activity
Na+
-
1.25 mM, 4% inhibition. 10 mM, 20% inhibition
Na+
activating at 1 mM, inhibitory at 10 mM
Na+
-
98% residual activity at 100 mM
Na+
-
1 mM, 4% inhibition
Na+
56.6% residual activity at 10 mM
Na2CO3
-
100 mM, 71% inhibition
NaCl
-
90% inhibition at 0.3 M, completely reversible
NaCl
-
mutant enzyme V159E/N398D
NaCl
10 mM, 20% inhibition. 500 mM, 63% inhibition. 1000 mM, complete loss of activity
NaCl
1000 mM, 24% inhibition
NaCl
-
500 mM, 52-59% inhibition (depending on purification method)
NaCl
-
10 mM, 17% inhibition
NaCl
-
100 mM, 51% inhibition
NaCl
-
80% inhibition at 50 mM
NaCl
-
50% inhibition at 20 mM, the inhibitory effect of chloride ions is reversible
NaCl
-
1 M, 90% inhibition
NaF
-
mutant enzyme V159E/N398D
NaF
-
0.1 mM, 75% inhibition
NaF
-
47% inhibition at 10 mM
NaF
-
complete inhibition at over 10 mM with substrates 3,6-dimethoxyphenol and 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
NaF
-
53% inhibition at 0.1 mM
NaN3
-
1 mM, 60% inhibition
NaN3
1 mM, 35% inhibition
NaN3
-
10 mM, 68% loss of activity
NaN3
complete inhibition at 5 mM
NaN3
-
0.1 mM, complete inhibition
NaN3
competitive, complete inhibition
NaN3
-
1% residual activity at 10 mM
NaN3
-
complete inhibition at 2 mM
NaN3
-
complete inhibition at 0.1 mM
NaN3
-
complete inhibition
NaN3
-
1 mM, 98% inhibition
NaN3
-
1 mM, complete loss of activity
NaN3
-
0.1 mM, complete inhibition
NaN3
8.9% residual activity at 1 mM
NaN3
-
completely inhibited by 50 mM
NaN3
-
7.86% inhibition at 10 mM
NaN3
-
1 mM, complete loss of activity
NaN3
-
22% inhibition at 10 mM, no inhibition by 1 mM NaN3
NaN3
10 mM, residual activity of wild-type enzyme is 23%, residual activity of mutant enzyme M199A is 13%, residual activity of mutant enzyme M199G is 20%, residual activity of mutant enzyme Y230A is 20%, residual activity of mutant enzyme H165A/M199G is 1116%, residual activity of mutant enzyme H165A/R240H is 78%, residual activity of mutant enzyme M199G/R240H is 25%
NaN3
-
1 mM, 5.5% loss of activity
NaN3
MK290990.1
100 mM, 96.28% residual activity
NaN3
-
0.5 mM, 85% inhibition
NaN3
-
complete inhibition at over 80 mM with substrate 3,6-dimethoxyphenol and at over 40 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
NaN3
competitive inhibition
NaN3
-
complete inhibition at 0.1 mM
NaN3
-
77% inhibition at 0.01 mM
NaN3
-
complete inhibition at 0.1 mM
Ni2+
20 mM, weak inhibitory effect
Ni2+
-
98.4% residual activity at 10 mM
Ni2+
-
5 mM, 25% inhibition
Ni2+
-
62% residual activity at 20 mM
Ni2+
-
32% inhibition at 1 mM
Ni2+
-
10 mM, 68% inhibition
Ni2+
-
1 mM, 57% inhibition
Ni2+
-
100 mM, 52% inhibition
Ni2+
-
3 mM, 33% inhibition
Ni2+
-
5 mM, 35% loss of activity
oxalic acid
-
p-coumaric acid
-
-
p-coumaric acid
5 mM, 60% inhibition
p-coumaric acid
-
1 mM, 25% inhibition
p-coumaric acid
-
1 mM, 48% inhibition
Pb2+
-
10 mM, 9% inhibition
Pb2+
-
97.2% residual activity at 20 mM
Pb2+
-
inhibits 50% at 10 mM
Pb2+
-
10 mM, 56% loss of activity
phenylmercuric acetate
-
-
phenylmercuric acetate
-
-
phenylmercuric acetate
-
-
potassium cyanide
-
90% at 1 mM
potassium cyanide
-
50% at 0.6 mM
potassium cyanide
-
87% at 0.1 mM
potassium cyanide
-
66-73% at 0.1 mM
SDS
-
SDS
-
mutant enzyme V159E/N398D
SDS
-
0.05 mM, 41% loss of activity
SDS
-
1 mM, 38.6% loss of activity
SDS
-
1 mM, 37-41% inhibition (depending on purification method)
SDS
0.1 mM, activity is reduced to 11.4%
SDS
-
5 mM, 46% inhibition
SDS
competitive, complete inhibition
SDS
-
93.8% residual activity at 20 mM
SDS
-
1.6fold activation at 1 mM, 89% inhibition at 10 mM
SDS
-
1 mM, 19% inhibition
SDS
-
10 mM, 89% inhibition
SDS
-
1 mM, 43% inhibition
SDS
-
10 mM, 21% inhibition
SDS
-
complete inhibition at 140 mM
SDS
-
3 mM, 85% inhibition
SDS
-
completely inhibited by 32 mM
SDS
-
10.2% inhibition at 10 mM
SDS
Sporothrix carnis
-
1 mM, 27% inhibition
SDS
-
1 mM, 20% loss of activity
SDS
MK290990.1
100 mM, 69.93% residual activity
SDS
-
10 mM, 15% inhibition
SDS
-
1 mM, 83.33% inhibition
SDS
-
40% inhibition at 10 mM, 10% at 1 mM
SDS
-
0.5%, 60% loss of activity
Sodium azide
-
Sodium azide
-
1 mM, 98% inhibition
Sodium azide
-
50% inhibition at 0.009 mM
Sodium azide
-
0.05 mM, 50% loss of activity
Sodium azide
-
completely inhibits enzyme activity
Sodium azide
0.1 mM, activity is reduced to 9.2%
Sodium azide
0.05 M, complete inhibition
Sodium azide
-
0.1 mM, complete inhibition
Sodium azide
strong inhibition
Sodium azide
-
1 mM, 12% inhibition
Sodium azide
1 mM, 33.8% loss of activity
Sodium azide
-
complete inhibition of laccase activity at 0.1% concentration
Sodium azide
-
0.1 mM, 97% inhibition
Sodium azide
-
0.5 mM, 94% inhibition
Sodium azide
-
30% inhibition
Sodium azide
Sporothrix carnis
-
1 mM, 27% inhibition
Sodium azide
-
5 mM, 40% inhibition
Sodium azide
-
1 mM, 68% residual activity
Sodium azide
Thermochaetoides thermophila
-
0.05 mM, 84% inhibition
Sodium azide
-
1 mM, 91.4% inhibition
Sodium azide
-
strong inhibition at 0.1 mM
Sodium azide
0.1 mM, strong
Sodium cyanide
-
1 mM, 0% residual activity
Sodium cyanide
-
1 mM, 81.18% inhibition
sodium dodecylsulfate
-
sodium dodecylsulfate
-
50% inhibition at 1 mM
sodium dodecylsulfate
-
50% inhibition at 1%
sodium dodecylsulfate
-
20-70% inhibition at 8 mM
sodium thiosulfate
-
sodium thiosulfate
1 mM, 98% inhibition
thioglycolic acid
-
1 mM, 82% inhibition
thioglycolic acid
-
50% inhibition at 0.5 mM
thioglycolic acid
-
2.5 mM, 50% loss of activity
thioglycolic acid
-
mixed competitive inhibition after a lag-phase
thioglycolic acid
strong inhibition
thioglycolic acid
-
93% inhibition at 0.1 mM, complete inhibition at 1 mM
thioglycolic acid
-
85% inhibition at 1 mM
thioglycolic acid
Thermochaetoides thermophila
-
0.1 mM, 91% inhibition
thioglycolic acid
-
inhibits 92.4% at 750 mM
thioglycolic acid
-
1 mM, 100% inhibition
Thiourea
-
1 mM, 60% inhibition
Thiourea
-
1 mM, complete inhibition
Thiourea
1 mM, 4.5% loss of activity. 10 mM, 90.4% loss of activity
Thiourea
-
73% inhibition at 5 mM
Thiourea
Thermochaetoides thermophila
-
-
tropolone
-
1 mM, 78% inhibition
tropolone
9.7% residual activity at 1 mM
Urea
-
Urea
Sporothrix carnis
-
1 mM, 21% inhibition
Urea
-
0.4 mM, complete inhibition
Urea
inactivation at 5 M, 50% inhibition at about 2 M
Zn2+
-
10 mM, 6% inhibition
Zn2+
partially inhibits laccase activity
Zn2+
-
10 mM, 13% inhibition
Zn2+
activating at 1 mM, inhibitory at 10 mM
Zn2+
strongly inhibits at 10 mM
Zn2+
-
75.6% residual activity at 10 mM
Zn2+
-
1 mM, reduces LacG activity to 48%
Zn2+
-
22% inhibition at 100 mM
Zn2+
-
10 mM, 88% inhibition
Zn2+
-
50% inhibition at 50 mM
Zn2+
65.7% residual activity at 1 mM
Zn2+
-
inhibits 40% at 10 mM
Zn2+
-
3 mM, 66% inhibition
Zn2+
-
10 mM, about 30% inhibition
Zn2+
-
200 mM, 85.8% residual activity
Zn2+
-
19% inhibition at 5 mM
Zn2+
-
50 mM, 97% loss of activity
ZnSO4
-
10 mM, 0.5% inhibition
ZnSO4
MK290990.1
1 mM, 30 min, 84.52% residual activity
additional information
Mn2+ even at 20 mM does not affect Atm activity
-
additional information
no inhibition: EDTA (10-50 mM), SDS (0.1-1 mM)
-
additional information
-
no inhibition: EDTA (10-50 mM), SDS (0.1-1 mM)
-
additional information
-
the enzyme can tolerate up to 600-800 mM NaCl. NaN3 (1 mM) has almost no effect on the laccase activity
-
additional information
no significant loss of activity loss at 10 mM EDTA
-
additional information
except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity
-
additional information
-
except for copper ions, most metal ions inhibit the laccase activity at a high concentration of about 10 mM, EDTA has no inhibitory effect on the laccase activity
-
additional information
isoenzyme Lac4 is resistant to SDS, and retains 43.08% activity in the presence of 100 mM SDS; isoenzymes Lac3 is resistant to SDS, and retains 31.86% activity in the presence of 100 mM SDS
-
additional information
isoenzyme Lac4 is resistant to SDS, and retains 43.08% activity in the presence of 100 mM SDS; isoenzymes Lac3 is resistant to SDS, and retains 31.86% activity in the presence of 100 mM SDS
-
additional information
-
isoenzyme Lac4 is resistant to SDS, and retains 43.08% activity in the presence of 100 mM SDS; isoenzymes Lac3 is resistant to SDS, and retains 31.86% activity in the presence of 100 mM SDS
-
additional information
-
5 mM potassium cyanide shows no inhibitory effect on the laccase
-
additional information
no inhibition by EDTA
-
additional information
-
not inhibitory: EDTA. L-cysteine
-
additional information
-
no inhibition by 0.1 mM EDTA, laccase retains 100 % activity with 2,6-dimethoxyphenol as a substrate in the presence of clorgiline or pargyline (specific amine oxidase inhibitors)
-
additional information
the recombinant His-tagged MmPPOA-695 shows a strong resistance to NaCl, DMSO, and Tween-80
-
additional information
-
the recombinant His-tagged MmPPOA-695 shows a strong resistance to NaCl, DMSO, and Tween-80
-
additional information
FJ560721
poor inhibition by NaF and NaBr
-
additional information
-
no inhibition by iodoacetate and chloroacetamide
-
additional information
-
no inhibition by iodoacetate and chloroacetamide
-
additional information
-
no inhibition: SDS, NaBr
-
additional information
-
EDTA and DMSO have almost no inhibitory effect
-
additional information
-
no inhibition by EDTA at 1-10 mM
-
additional information
the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF
-
additional information
-
the enzyme is resistant to specific inhibitors of copper-containing oxidases, such as NaN3 and NaF
-
additional information
-
no inhibition by iodoacetate and chloroacetamide
-
additional information
Thermochaetoides thermophila
-
strongly inhibited by Cu-chelating agents
-
additional information
-
SDS and EDTA did not inhibit enzyme activity
-
additional information
-
no inhibition by DMSO up to 25 mM
-
additional information
-
study on inhibitors and inhibition
-
additional information
-
no or poor inhibition by Co2+, Zn2+, Sn2+, Mn2+, Mg2+, K+, Fe2+, Ba2+, and Na+
-
additional information
-
soluble laccase shows more than 80% relative activity in organic solvents like ethanol, methanol, toluene and acetonitrile
-
1.10.3.2 laccase
results (
results (
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