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1.10.3.2: laccase

This is an abbreviated version!
For detailed information about laccase, go to the full flat file.

Word Map on EC 1.10.3.2

Reaction

4 benzenediol +

O2
= 4 benzosemiquinone + 2 H2O

Synonyms

ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.2 laccase

Storage Stability

Storage Stability on EC 1.10.3.2 - laccase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.01 M phosphate buffer, pH 7.0, at least 1 year
-
-20°C, 0.1 M phosphate buffer, pH 5
-
-20°C, several months stable
-
0-4°C, pH 5.0-7.0, concentrated or lyophilized enzyme, stable for 4 months
-
20°C, 2 days, pH 6.0-10.0, the enzyme retains about 70% residual activity
2°C, several weeks stable
-
40°C, 24 h, concentrated or lyophilized enzyme, stable in presence of 1% acetonitrile
-
4°C, 8 days, soluble enzyme loses 45% of its activity. Sol–gel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively
-
4°C, accase retained more than 50% activity in the phosphate-buffered saline, stable for 1 week
4°C, immobilized, 2 years, no loss of activity
-
4°C, in deionized water, stable for more than 20 days
4°C, pH 4.5-8.0, stable for several days
-
4°C, pH 7, several days, or frozen several months stable
-
4°C, purified enzyme, 50 mM citrate phosphate, pH 5.0, no loss in activity after 1 month
4°C, purified isozymes in 10 mM Na acetate buffer, pH 5.0, isozyme Lacc II is stable for at least 6 months, isozyme Lacc I shows 40% remaining activity after 6 months
-
5°C, glass-distilled water, pH 5.5, stable
-
highest recovery of enzymatic activity after freezing and thawing is observed by storing both laccase in 50 mM sodium phosphate buffer at pH 8.0 with the addition of 30 % (w/v) glycerol and 3.0 % sucrose
the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4
-