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1.11.1.14: lignin peroxidase

This is an abbreviated version!
For detailed information about lignin peroxidase, go to the full flat file.

Word Map on EC 1.11.1.14

Reaction

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol
+
H2O2
=
3,4-dimethoxybenzaldehyde
+
2-methoxyphenol
+
glycolaldehyde
+
H2O

Synonyms

ALiP-P3, bacterial lignin peroxidase, diarylpropane oxygenase, diarylpropane peroxidase, diarylpropane:oxygen,hydrogen-peroxide oxidoreductase (C-C-bond-cleaving), DypB, fungal lignin peroxidase, Glg4, H2O2-dependent ligninase, heme-containing lignin peroxidase, heme-containing peroxidase, lignin peroxidase, lignin peroxidase H8, lignin peroxidase isozyme H8, lignin peroxidase LIII, ligninase, ligninase H2, ligninase H8, ligninase I, ligninase LG5, LIP, Lip1, LIP2, LiPH8, lipJ, LPA, LPOA, microbial lignin peroxidase, More, mushroom tyrosinase, oxygenase, diarylpropane, Pr-lip1, Pr-lip4

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.14 lignin peroxidase

Purification

Purification on EC 1.11.1.14 - lignin peroxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
10 kDa ultrafiltration, 3 M ammonium sulphate precipitation, hydrophobic interaction chromatography, reversed phase chromatography
-
16 isozymes: TvLP1-16, differing in primary structure
-
3 isozymes by ion-exchange chromatography
-
active solubilized recombinant enzyme expressed from Escherichia coli strain W3110 is purified by anion exchange chromatography using a 0-300 mM NaCl gradient (20 min, 2 ml/min flow rate) in 10 mM sodium tartrate, pH 5.5, containing 1 mM CaCl2
at least 7 isozymes, LiP-1-7
-
DEAE-cellulose column chromatography
-
extracellular enzyme partially from strain NK-1 by ammonium sulfate fractionation and gel filtration
-
extracellular native enzyme by ultrafiltration and anion exchange chromatography, followed by ultrafiltration and gel filtration
-
ion exchange chromatography, 4°C
-
native extracellular enzyme 21fold by immobilization on polyurethane foam cubes, ammonium sulfate fractionation, ultrafiltration, and gel filtration
-
native extracellular enzyme partially by ammonium sulfate fractionation and dialysis
-
purification from culture supernatant
-
recombinant His-tagged N246A mutant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Resource Q column chromatography, gel filtration
salt precipitation with 60% (NH4)2SO4, desalting column, Q FF ion exchange column and Sepharyl S-300 HR gel filtration
-
to homogeneity using Amicon concentration and DEAE cellulose chromatography
Loweporus lividus
-
ultrafiltration, anion-exchange and size exclusion chromatography