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1.11.1.14: lignin peroxidase

This is an abbreviated version!
For detailed information about lignin peroxidase, go to the full flat file.

Word Map on EC 1.11.1.14

Reaction

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol
+
H2O2
=
3,4-dimethoxybenzaldehyde
 +
2-methoxyphenol
 +
glycolaldehyde
 +
H2O

Synonyms

ALiP-P3, bacterial lignin peroxidase, diarylpropane oxygenase, diarylpropane peroxidase, diarylpropane:oxygen,hydrogen-peroxide oxidoreductase (C-C-bond-cleaving), DypB, fungal lignin peroxidase, Glg4, H2O2-dependent ligninase, heme-containing lignin peroxidase, heme-containing peroxidase, lignin peroxidase, lignin peroxidase H8, lignin peroxidase isozyme H8, lignin peroxidase LIII, ligninase, ligninase H2, ligninase H8, ligninase I, ligninase LG5, LIP, Lip1, LIP2, LiPH8, lipJ, LPA, LPOA, microbial lignin peroxidase, More, mushroom tyrosinase, oxygenase, diarylpropane, Pr-lip1, Pr-lip4

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.14 lignin peroxidase

Temperature Stability

Temperature Stability on EC 1.11.1.14 - lignin peroxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 40
10 - 70
23
-
retains nearly 30% of initial activity after 18 h, in presence of veratryl alcohol
25
-
pH 7.0, 4 h, maximum activity loss of 10%
25 - 37
the recombinant enzyme maintains a large part of the starting activity following incubation for 24 h in this temperature range
25 - 75
-
pH 5, 100% lignin peroxidase activity is retained at 55°C after incubation. The lignin peroxidase activity drops more than 75% at temperatures below 35°C and loses about 66% of its activity at a temperature of 75°C
30 - 60
the recombinant enzyme is fully stable up to 30°C but unstable at 37°C, and is completely inactivated after 30 min at 60°C
40
21 h, pH 4.0-6.5, 80% residual activity
45
-
after 2 h incubation at 45°C, 55% of the initial activity of the immobilized lignin peroxidase (on nanoporous gold) is still retained while the free lignin peroxidase is completely deactivated, pH 3.5 citrate buffer
50
stable for 48 h up to 50°C
60
6 h, complete loss of activity
60 - 80
-
the purified enzyme exhibits a higher thermostability and retains over 50% of the activity, even after 120 min of incubation at both 60°C and 65°C. Loss of 25% of the activitiy after 120 min at 70°C. 25% activity is maintained after 100 min at both 75°C and 80°C and activity is completely lost after 120 min
63 - 65
the recombinant enzyme shows a good thermostability with a melting temperature of 63-65°C
70
min, complete loss of activity
additional information
an extra disulfide and ionic salt bridge improves the thermostability of lignin peroxidase H8 under acidic condition. Native LiP under mild conditions with half-life (t1/2) of 8.2 days at pH 6.0 exhibits a marked decline in thermostability under acidic conditions with t1/2 of only 14 min at pH 2.5