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1.12.2.1: cytochrome-c3 hydrogenase

This is an abbreviated version!
For detailed information about cytochrome-c3 hydrogenase, go to the full flat file.

Word Map on EC 1.12.2.1

Reaction

H2
+ 2 ferricytochrome c3 = 2 H+ + 2 ferrocytochrome c3

Synonyms

cytochrome c3 hydrogenase, cytochrome c3 reductase, cytochrome hydrogenase, cytochrome-c3 hydrogenase, Fe-only hydrogenase, H2:ferricytochrome c3 oxidoreductase, H2ase, hyd B, HydAB, hydrogenase, hydrogenase, cytochrome, Hyn-1B, Hyn1 hydrogenase, HynAB-1, HysAB, [FeFe] hydrogenase, [Fe] hydrogenase, [Fe]-hydrogenase, [NiFeSe] Hase, [NiFeSe] hydrogenase, [NiFe] Hase, [NiFe] hydrogenase, [NiFe] hydrogenase 1, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.2 With a cytochrome as acceptor
                1.12.2.1 cytochrome-c3 hydrogenase

Temperature Stability

Temperature Stability on EC 1.12.2.1 - cytochrome-c3 hydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
10 min, H2 evolution in presence of methyl viologen, complete loss of activity
23
-
atmosphere of N2, air or H2, 40% loss of activity after 2 weeks, complete loss of activity after 4 weeks
35 - 40
-
at optimal temperature range of 35-40°C, a minimal activity loss during the first 10 to 20 min and thereafter, activity steadily decreases as incubation time increases. After 80 min of incubation, the enzyme loses 75-80% of all of its activity
4
-
4 weeks, atmosphere of N2, air or H2, very little loss of activity
50
-
10 min, H2 evolution in presence of methyl viologen, no decrease in activity
55
-
completely denatured above
60
-
10 min, H2 evolution in presence of methyl viologen, 30% loss of activity
70
-
10 min, H2 evolution in presence of methyl viologen, 37% loss of activity
77
-
stable below, 2 h
90
-
10 min, H2 evolution in presence of methyl viologen, 99% loss of activity