all ehb mutants have severe growth deficiencies except the DeltaehbO::pac strain: The membrane-spanning ion translocator (DELTAehbF) and the large hydrogenase subunit (DELTAehbN) deletion strains display the severest growth defects. The DELTAehbN mutant strain is sensitive to growth inhibition by aryl acids
the Ech hydrogenase deletion mutant shows 50% decreased ferredoxin-dependent membrane-bound electron transport activity, a lower growth rate, and 2fold faster substrate (trimethylamine) consumption compared to the wild type enzyme
Ech H2 evolving activity is only delayed when hyp1 is deleted. Deletion of hyp1FCDE alone still results in H2 production and CO and H2 uptake, albeit in a delayed manner compared to wild-type
hyp2 deletion completely disrupts MBH H2 uptake. When both hyp1FCDE and hyp2 are deleted (hyp1/2DELTA), there is no significant CO uptake and no H2 detected, indicating that the maturation and activity of both hydrogenases is likely disrupted. At least some of the absent Hyp2 proteins, and not HypA1 and HypB1 alone, are required for rescue of maturation of the Ech hydrogenase
Ech H2 evolving activity is only delayed when hyp1 is deleted. Deletion of hyp1FCDE alone still results in H2 production and CO and H2 uptake, albeit in a delayed manner compared to wild-type
hyp2 deletion completely disrupts MBH H2 uptake. When both hyp1FCDE and hyp2 are deleted (hyp1/2DELTA), there is no significant CO uptake and no H2 detected, indicating that the maturation and activity of both hydrogenases is likely disrupted. At least some of the absent Hyp2 proteins, and not HypA1 and HypB1 alone, are required for rescue of maturation of the Ech hydrogenase
photosynthetic induction of Chlamydomonas reinhardtii in anaerobic conditions is facilitated by the presence of hydrogenase as transient electron sink. Lack of hydrogenase activity in a state 1 locked mutant is detrimental to its ability to induce photosynthesis in an anaerobic environment
Ech hydrogenase acts as primary proton pump in a ferredoxin-dependent electron transport system. Ech hydrogenase is necessary to generate an electrochemical ion gradient when ferredoxin is the only reducing equivalent and heterodisulfide is absent
Ech hydrogenase acts as primary proton pump in a ferredoxin-dependent electron transport system. Ech hydrogenase is necessary to generate an electrochemical ion gradient when ferredoxin is the only reducing equivalent and heterodisulfide is absent