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1.12.7.2: ferredoxin hydrogenase

This is an abbreviated version!
For detailed information about ferredoxin hydrogenase, go to the full flat file.

Word Map on EC 1.12.7.2

Reaction

H2
+
oxidized ferredoxin
=
reduced ferredoxin
+ 2 H+

Synonyms

bidirectional hydrogenase, C-MBH, CooH, CpI, DDH, DdHydAB, EC 1.12.1.1, EC 1.12.7.1, EC 1.18.3.1, EC 1.18.99.1, EC 1.98.1.1, Ech hydrogenase, EHb, EhbM, EhbN, energy-conserving hydrogenase B, energy-converting hydrogenase, Fe hydrogenlyase, Fe-Fe hydrogenase, Fe-H2ase, Fe-hydrogenase, ferredoxin-dependent [FeFe] hydrogenase, ferredoxin-dependent [FeFe]-hydrogenase, H2 oxidizing hydrogenase, H2 producing hydrogenase [ambiguous], HYD1, HYD2, hydA, HydA1, HydA2, hydrogen-lyase [ambiguous], hydrogenase (ferredoxin), hydrogenase I, hydrogenase II, hydrogenase of the Fe-only type, hydrogenlyase, hydrogenlyase [ambiguous], Mbh, MBH hydrogenase, MbhJ, MbhJKLN, MbhK, MbhL, MDH, membrane bound NiFe hydrogenase, membrane-bound hydrogenase, NiFe hydrogenase, reversible Fe-hydrogenase, uptake hydrogenase [ambiguous], [FeFe] hydrogenase, [FeFe] hydrogenase HydA1, [FeFe]-hydrogenase, [Fe]-hydrogenase, [Ni-Fe] hydrogenase, [NiFeSe] Hase, [NiFeSe] hydrogenase

ECTree

     1 Oxidoreductases
         1.12 Acting on hydrogen as donor
             1.12.7 With an iron-sulfur protein as acceptor
                1.12.7.2 ferredoxin hydrogenase

Purification

Purification on EC 1.12.7.2 - ferredoxin hydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1284fold with a yield of 16.17%, by ultracentrifugation, ion exchange chromatography, gel filtration and hydrophobic-interaction chromatography
-
aerobically purified hydrogenase is inactive and requires reductive activation
-
aerobically using different ion-exchange chromatography columns
-
affinity column chromatography with immobilised ferredoxin
-
Ni-NTA column chromatography
-
Ni-Sepharose 6 Fast Flow chromatography
-
partially
partially, 40% pure
-
Procion Red-agarose purification
purification of the engineered and expressed His-tagged 4-subunit cytoplasmic subcomplex of respiratory membrane-bound hydrogenase
purified under aerobic conditions, requires reductive activation
-
Q-sepharose HiLoad, hydroxyapatite, Superdex 200
-
Strep-Tactin-Sepharose column chromatography and Superdex 200 gel filtration
-
strictly under anaerobic conditions
-
under anaerobic conditions, solubilized with n-dodecyl-beta-D-maltoside
-