1.12.98.1: coenzyme F420 hydrogenase

This is an abbreviated version!
For detailed information about coenzyme F420 hydrogenase, go to the full flat file.

Reaction

Synonyms

8-hydroxy-5-deazaflavin-reactive hydrogenase, 8-hydroxy-5-deazaflavin-reducing hydrogenase, coenzyme F420-dependent hydrogenase, coenzyme F420-reducing dehydrogenase, deazaflavin-reducing hydrogenase, EC 1.12.99.1, F420-reducing hydrogenase, F420-reducing [NiFe] hydrogenase, F420-reducing [NiFe]-hydrogenase, F420H2 dehydrogenase, Fpo, FRH, FrhABC, FrhABG, frhAGB-encoded hydrogenase, hydrogen:(acceptor) oxidoreductase, Mbar_A0449, Mbar_A0450, Mbar_A0452, TON_1559, TON_1560, TON_1561, VhuD

ECTree

     1 Oxidoreductases

         1.12 Acting on hydrogen as donor

             1.12.98 With other, known, physiological acceptors

                1.12.98.1 coenzyme F420 hydrogenase

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Results	in table
8	Activating Compound
1025	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
25	Cofactor
1	Crystallization (Commentary)
1	Expression
11	General Information
4	General Stability
5	Inhibitors
2	kcat/KM [mM/s]
1	Ki Value [mM]
20	KM Value [mM]
9	Localization
37	Metals/Ions
40	Molecular Weight [Da]
41	Natural Substrates/ Products (Substrates)
82	Organism
5	Oxidation Stability
6	Pathway
14	PDB ID
8	pH Optimum
5	pH Range
1	pH Stability
1	pI Value
2	Protein Variants
18	Purification (Commentary)
1	Reaction
3	Reaction Type
43	Reference
24	Specific Activity [micromol/min/mg]
6	Storage Stability
97	Substrates and Products (Substrate)
26	Subunits
39	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
10	Turnover Number [1/s]

Oxidation Stability

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Oxidation Stability on EC 1.12.98.1 - coenzyme F420 hydrogenase

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OXIDATION STABILITY

ORGANISM

UNIPROT

LITERATURE

aerobic purification yields the stable but inactive enzyme, can be reactivated under reducing conditions

Methanothermobacter thermautotrophicus

-

395086

enzyme activity is unstable under reducing conditions

Methanosarcina barkeri

P80490; P80489; P80491

727399

extremely oxygen sensitive, can be reactivated by incubation with molecular hydrogen and dithiothreitol

Methanococcus vannielii

-

395079

more stable under aerobic conditions, reversible loss of activity with oxygen can be restored with dithionite

Methanobacterium formicicum

-

395082

purified enzyme requires reductive reactivation before assay, reactivation possible with H2 or glucose and glucose oxidase

Methanobacterium formicicum

-

395093