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1.13.11.1: catechol 1,2-dioxygenase

This is an abbreviated version!
For detailed information about catechol 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.1

Reaction

catechol
+
O2
=
cis,cis-muconate

Synonyms

(chloro-)catechol 1,2-dioxygenase, 1,2 CTD, 1,2-CTD, 1,2-pyrocatechase, 3-chlorocatechol 1,2-dioxygenase, BC-C23O, C1,2-DO, C1,2O, C12, C12D, C12DO, C12O, cat12, CatA, catechase, catechol 1,2 dioxygenase, catechol 1,2-dioxygenase, catechol 1,2-dioxygenase type I, catechol 1,2-dioxygenase type II, catechol 1,2-oxygenase, catechol dioxygenase, catechol oxygenase, catechol-oxygen 1,2-oxidoreductase, catechol:oxygen 1,2-oxidoreductase, CD I, CD II, CD-I, CD-II, CD-III-1, CD-III-2, CDI1, CDI2, chlorocatechol 1,2-dioxygenase, clCA, extradiol dioxygenase MhpB, IsoB, putative catechol 2,3-dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, TfdC

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.1 catechol 1,2-dioxygenase

Crystallization

Crystallization on EC 1.13.11.1 - catechol 1,2-dioxygenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, at 4°C
alpha alpha isoenzyme: orthorhombic space group C222 1, unit cell dimensions a = 62.7 A, b = 71.5 A, c = 187.1 A
-
hanging drop method, 2.65 A resolution
-
sitting drop vapor diffusion method, using 30% (w/v) PEG400, 0.1 M MES pH 6.5, 0.1 M magnesium chloride
sitting-drop vapour-difusion method, 1.9 A resolution
sitting-drop vapour-diffusion technique, X-ray characterization, space group C2, unit-cell dimensions: a = 111.9 a, b = 78.1 A, c = 134.6 A, beta = 100°
-