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1.13.11.1: catechol 1,2-dioxygenase

This is an abbreviated version!
For detailed information about catechol 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.1

Reaction

catechol
+
O2
=
cis,cis-muconate

Synonyms

(chloro-)catechol 1,2-dioxygenase, 1,2 CTD, 1,2-CTD, 1,2-pyrocatechase, 3-chlorocatechol 1,2-dioxygenase, BC-C23O, C1,2-DO, C1,2O, C12, C12D, C12DO, C12O, cat12, CatA, catechase, catechol 1,2 dioxygenase, catechol 1,2-dioxygenase, catechol 1,2-dioxygenase type I, catechol 1,2-dioxygenase type II, catechol 1,2-oxygenase, catechol dioxygenase, catechol oxygenase, catechol-oxygen 1,2-oxidoreductase, catechol:oxygen 1,2-oxidoreductase, CD I, CD II, CD-I, CD-II, CD-III-1, CD-III-2, CDI1, CDI2, chlorocatechol 1,2-dioxygenase, clCA, extradiol dioxygenase MhpB, IsoB, putative catechol 2,3-dioxygenase, pyrocatechase, pyrocatechol 1,2-dioxygenase, TfdC

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.1 catechol 1,2-dioxygenase

Temperature Stability

Temperature Stability on EC 1.13.11.1 - catechol 1,2-dioxygenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
for 10 min, stable activity of CD I, activity of CD II decrease to 89%
37 - 55
-
the enzyme is stable at the optimal temperature at 37°C for 5 h and loses 10% of its activity after 2 h. As the temperature increases, the enzyme activity declines (37% of activity remains at 45°C under the assay conditions, while the enzyme loses approximately 90% activity in 1 h at 50 and 55°C, respectively)
4
-
2 month: 100% enzyme activity left
40 - 45
Stutzerimonas stutzeri
1 h, no loss of activity
43
-
30 min, 18% loss of activity
55
-
half-life of the enzyme from benzoate-grown cells is 27 min, half-life of the enzyme from p-toluate-grown cells is 14 min