1.13.11.15: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

This is an abbreviated version!
For detailed information about 3,4-dihydroxyphenylacetate 2,3-dioxygenase, go to the full flat file.

Reaction

Synonyms

2,3-HPCD, 3,4-dihydroxyphenylacetic acid 2,3-dioxygenase, Bf 2,3-HPCD, DHPAO, Fe-HPCD, Fe-MndD, FeHPCD, homoprotocatechuate 2,3 dioxygenase, homoprotocatechuate 2,3-dioxygenase, homoprotocatechuate dioxygenase, HPADO, HPC 2,3-dioxygenase, HPC dioxygenase, HPCA 2,3-dioxygenase, HPCD, Mn(II)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenase, Mn-HPCD, Mn-MndD, MndD, MnHPCD, oxygenase, homoprotocatechuate 2,3-di-, PaDHPAO

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.15 3,4-dihydroxyphenylacetate 2,3-dioxygenase

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Results	in table
3	Activating Compound
2227	AA Sequence
2	Application
1	CAS Registry Number
8	Cloned(Commentary)
8	Crystallization (Commentary)
2	Expression
7	General Information
4	General Stability
46	Inhibitors
2	kcat/KM [mM/s]
9	Ki Value [mM]
40	KM Value [mM]
41	Metals/Ions
16	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
84	Organism
6	Pathway
72	PDB ID
18	pH Optimum
2	pH Range
2	pH Stability
29	Protein Variants
15	Purification (Commentary)
4	Reaction
3	Reaction Type
60	Reference
3	Source Tissue
8	Specific Activity [micromol/min/mg]
2	Storage Stability
102	Substrates and Products (Substrate)
14	Subunits
49	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
6	Temperature Stability [°C]
22	Turnover Number [1/s]

Expression

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Expression on EC 1.13.11.15 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

induction of fumarylacetoacetate hydrolase HmgB in Burkholderia xenovorans LB400 during growth on hydroxyphenylacetates

Paraburkholderia xenovorans

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726271

LB400 cells grown with 3-hydroxyphenylacetate and 4-hydroxyphenylacetate both show induction of the homoprotocatechuate pathway, they degrade homoprotocatechuate and show homoprotocatechuate 2,3-dioxygenase activity. The hmgA1 gene is upregulated by 3-hydroxyphenylacetate in strain LB400. The expression of the hpaD gene, encoding HPC 2,3-dioxygenase, is induced in 3-hydroxyphenylacetate and 4-hydroxyphenylacetate grown-cells

Paraburkholderia xenovorans

-

726271