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1.13.11.33: arachidonate 15-lipoxygenase

This is an abbreviated version!
For detailed information about arachidonate 15-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.33

Reaction

arachidonate
+
O2
=
(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate

Synonyms

12/15 lipoxygenase, 12/15 Lox, 12/15-lipoxygenase, 12/15-LO, 12/15-LOX, 12/15LO, 15(S)-lipoxygenase-1, 15-hLO, 15-hLO-1, 15-hLO-2, 15-lipoxygenase, 15-lipoxygenase 1, 15-lipoxygenase 2, 15-lipoxygenase type 1, 15-lipoxygenase type 2, 15-lipoxygenase type-1, 15-lipoxygenase-1, 15-lipoxygenase-2, 15-lipoxygenase-I, 15-LO, 15-LO-1, 15-LO-2, 15-LO-I, 15-LOX, 15-LOX type 1, 15-LOX-1, 15-LOX-2, 15-LOX-A, 15-LOX2, 15-rLO, 15-rLO-1, 15-sLO, 15LO1, 15S-lipoxygenase, 15S-LOX1, ALOX12, Alox15, ALOX15-2, ALOX15B, arachidonate 12/15-lipoxygenase, arachidonate 15-lipoxygenase, arachidonate 15-lipoxygenase-1, arachidonic acid 15-lipoxygenase, arachidonic acid 15-lipoxygenase-1, cardiac 12/15-LOX, endothelial 15-lipoxygenase-1, epidermis-type 15-LOX, human prostate epithelial 15-lipoxygenase-2, linoleic acid omega-6-lipoxygenase, lipoxygenase L-1, lipoxygenase-1, LO-1, LOX-1, LOX2, LOX2:Hv:1, LoxA, More, omega-6 lipoxygenase, oxygenase, arachidonate 15-lip-, reticulocyte 15-lipoxygenase-1, reticulocyte 15-LOX, reticulocyte-type 15-human lipoxygenase, reticulocyte-type 15-lipoxygenase, reticulocyte-type 15-LO-1, reticulocyte-type of 15-LOX-1, SLO, soybean 15-lipoxygenase, soybean lipoxygenase-3

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.33 arachidonate 15-lipoxygenase

Cloned

Cloned on EC 1.13.11.33 - arachidonate 15-lipoxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
12/15LO stably overexpressed in mouse J774 macrophages
-
15-LO-1 expressed in Sf 9 cells
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15-LOX-1 cDNA fragment without 3'-UTR inserted into pLP-IRESneo, overexpression of 15-LOX-1 in A549 cells
-
15-LOX2 has at least six alternatively spliced isoforms
expressed as a fusion protein with GFP (pEGFP-15LOX-2) in DU145 and PC-3 cells
-
expressed in CV-1 cells
-
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli BL21 Star cells
expressed in Mus musculus C57BL/6
-
expressed in Mus musculus C57BL/6 prostate
-
expressed in rabbit aorta endothelium
-
expressed in Sf9 cells
-
expressed with hexa-His tags
-
expression in a human osteosarcoma cell line, expression of a CheY-15-lipoxygenase fusion protein in Escherichia coli
-
expression in CHO and in neuro-2 A neuroblastoma cells both stably expressing human APP carrying the K670N/M671L Swedish mutation, expression analysis
-
expression in Escherichia coli
expression in the baculovirus/insect cell system, expression as intracellular enzyme and after fusion with a signal peptide as export protein in High Five cells
-
expression of His-tagged isozyme 15-hLO-1, expression of isozyme 15-hLO-2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
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expression of isozyme 15-hLO-1 as His-tagged enzyme, and of untagged isozyme 15-hLO-2
-
expression of isozyme 15-hLO-2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, expression of N-terminally His6-tagged 15-hLO-1
-
expression to high levels, approximately 20% of cellular protein, in a baculovirus/insect cell expression system
-
gene ALOX15, DNA and amino acid sequence determination and analysis of wild-type and mutant T560M, promoter sequence analysis, genotyping, overview, expression of wild-type and mutant enzymes in Escherichia coli and HEK-297 cells, the mutant shows a low expression level in both systems compared to the wild-type enzyme
gene ALOX15, located on the short arm of chromosome 11
gene ALOX15, located on the short arm of chromosome 17
gene loxA, recombinant expression of N-terminally His6-tagged enzyme LoxA in Escherichia coli strain BL21(DE3)
HCT-116 and LoVo cells transfected with 15-LOX-1 vector
-
isoenzyme 15S-LOb and a splice variant of 15-LOb, baculovirus/insect cell expression system
-
ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue)
LOX isoforms ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue)
-
overexpressed as N-terminally, His6-tagged protein
-
overexpression of 15-LOX-2 in human macrophages by adenovirus-mediated gene delivery
-
overexpression of His-tagged enzyme in Escherichia coli
-
overexpression of isozyme 15-LO-1 in Oryctolagus cuniculus via adenovirus transfection method, the expression is restricted to aortic endothelial cells, overview
-
Pca cell clones stably expressing 15-LOX2 or splice variant 15-LOX2sc-b are estabilished. The 15-LOX2 clones express 15-LOX2 in the nuclei and possess robust enzymatic activity, whereas 15-LOX2sc-b clones show neither nuclear protein localization nor arachidonic acid-metabolizing activity
-
pcDNA3.1(1) vector carrying native or mutant 15-LOX-1 expressed in HCT-116 cells
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radiation-inducible 15-LOX-2 expression vector in which the full-length 15-LOX-2 cDNA is inserted downstream the recombinant Egr-1 promoter (pEgr-LOX2-GFP) and transfected in head-and-neck cancer cells
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RAW 267.4 murine macrophages stably transfected to overexpress 15-LO-1
-
recombinant expression of 15-LOX-1 in Spodoptera frugiperda SF9 cells
recombinant expression of His6-tagged isozyme 15-LOX-2 in Escherichia coli strain Rossetta 2 (DE3), coexpression with Escherichia coli YjgD protein
sequence comparisons and phylogenetic analysis
the recombinant wild-type enzyme and various mutants are expressed in Escherichia coli as His-tagged fusion proteins
transgenic mice models overexpressing 15-LOX in endothelial cells
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transgenic mice overexpressing 15-LO-1
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without a His6-tag
-