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1.13.11.4: gentisate 1,2-dioxygenase

This is an abbreviated version!
For detailed information about gentisate 1,2-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.4

Reaction

2,5-Dihydroxybenzoate
+
O2
=
maleylpyruvate

Synonyms

2,5-dihydroxybenzoate dioxygenase, DsmD, EC 1.13.1.4, EC 1.99.2.4, eGDO, GDO, GDO-II, gdoA, GDOsp, GenH, gentisate dioxygenase, gentisate oxygenase, gentisate-1,2-dioxygenase, gentisic acid oxidase, gtdA-2, hbzE, Nagl1, Nagl2, Nagl3, NarI, oxygenase, gentisate 1,2-di-, P25X gentisate 1,2-dioxygenase, P35X gentisate 1,2-dioxygenase, salicylate 1,2-dioxygenase, SDO

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.4 gentisate 1,2-dioxygenase

Cloned

Cloned on EC 1.13.11.4 - gentisate 1,2-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
deduced amino acid sequence contains 4 novel histidine clusters and a possible extradiol fingerprint region
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta cells
expressed in Escherichia coli strain BL21 (DE3)
expressed in Escherichia coli strain BL21/pET28ancg12920
-
expression in Escherichia coli
expression of wild-type and mutant enzymes
-
gene dbdB, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic analysis, overview
gene genH, DNA and amino acid sequence determination and analysis, gene genH belongs to the gen gene cluster, RT-PCR expression and genomic analysis, overview
gene narI, DNA and amino acid sequence determination and analysis of the gentisate catabolic gene cluster. Among the 13 complete open reading frames deduced from this fragment, three narIKL encode the enzymes involved in the reactions of gentisate catabolism, of these, NarI is gentisate 1,2-dioxygenase which converts gentisate to maleylpyruvate. Genes narI, narK, and narL genes are transcribed as a single operon only in the presence of naphthalene. Sequence comparisons, overview. Expression in Escherichia coli strain Rosetta (DE3) pLysS
genes nagI1, nagI2, and nagI3, the latter encoded in the third cluster, nagl3 is polycistronic and is expressed at a relatively high level, genetic organization, DNA and amino acid sequence determination and analysis, real-time RT-PCR for gene expression analysis of genes nagI1, nagI2 and nagI3 and the third catabolic gene cluster, overview. Overexpression of all three genes in Escherichia coli strain BL21(DE3). The transformation efficiency of the pET21bnagI1 ligation product is very low compared with that of the nagI2 and nagI3 genes
-
heterologously expressed in Escherichia coli as His-tagged enzyme
overexpressed as a hexahistidine fusion protein from Escherichia coli
-
overexpressed in Escherichia coli
-
overexpression of His6-tagged enzyme in Escherichia coli BL21(DE3)
-