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1.13.11.41: 2,4'-dihydroxyacetophenone dioxygenase

This is an abbreviated version!
For detailed information about 2,4'-dihydroxyacetophenone dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.41

Reaction

2,4'-dihydroxyacetophenone
+
O2
=
4-hydroxybenzoate
+
formate

Synonyms

(4-hydroxybenzoyl)methanol oxygenase, DAD, DHAP dioxygenase

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.41 2,4'-dihydroxyacetophenone dioxygenase

Cloned

Cloned on EC 1.13.11.41 - 2,4'-dihydroxyacetophenone dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene cloned, sequenced and expressed in Escherichia coli N-4830, nucleotide sequence of the gene dad deposited in the GenBank (r)/EMBL/DDBJ nucleotide sequence databases
gene dad, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain JM109
mutant enzyme V1M is expressed in Escherichia coli BL21(DE3) cells
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)