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1.13.11.52: indoleamine 2,3-dioxygenase

This is an abbreviated version!
For detailed information about indoleamine 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.52

Reaction

D-tryptophan
+
O2
=
N-formyl-D-kynurenine

Synonyms

31854, BRAFLDRAFT_126354, CG5163, EC 1.13.1.12, hIDO, hIDO1, hTDO, IDO, IDO-1, IDO-2, IDO-I, IDO-II, IDO-III, IDO-IV, IDO1, IDO2, INDO, INDOL1, indolamine 2,3-dioxygenase, indole 2,3-dioxygenase, indoleamine 2, 3-dioxygenase, indoleamine 2,3 dioxygenase, indoleamine 2,3-dioxygenase, indoleamine 2,3-dioxygenase 1, indoleamine 2,3-dioxygenase 2, indoleamine 2,3-dioxygenase-1, indoleamine 2,3-dioxygenase-2, indoleamine 2,3-dioxygenase-like protein, indoleamine-2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase, L-tryptophan 2,3-dioxygenase, L-tryptophan pyrrolase, mIDO, oxygenase, tryptophan 2,3-di-, proto-IDO, proto-indoleamine 2,3-dioxygenase, superoxygenase, TDO, TDO2, TioF, TO, TRPO, Tryptamin 2,3-dioxygenase, tryptamine 2,3-dioxygenase, tryptophan 2,3-dioxygenase, tryptophan dioxygenase, tryptophan oxygenase, tryptophan peroxidase, tryptophan pyrrolase, tryptophan-2,3-dioxygenase, tryptophanase, v1g244579, Vermilion protein

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.52 indoleamine 2,3-dioxygenase

Purification

Purification on EC 1.13.11.52 - indoleamine 2,3-dioxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
0-5°C, homogenization, Protein G-Sepharose
-
ammonium sulfate precipitation, hydroxyapatite column chromatography (two times), gel filtration, DEAE cellulose column chromatography, third hydroxyapatite column chromatography, Sephadex G-200 gel filtration, second DEAE cellulose column chromatography
-
cryosections deparaffinized, rehydrated, cooked in 0.01 M citrate buffer (pH 6.0), incubated with IDO antibody at room temperature for 30 min
-
dialysis, lyophilization
-
enzyme from intestine
-
expression of a fully functional enzyme in Escherichia coli
-
HisTrap column chromatography and Superdex 200 gel filtration
homogenization
-
homogenization, ammonium sulfate precipitation
-
homogenization, ammonium sulfate precipitation, gel filtration with Sephacryl S200, Bio-gel HT column, stored at -75°C
-
homogenization, filtered through cotton gauze, centrifugation, collection, stored at -20°C
-
IMAC nickel affinity column chromatography
Ni-NTA-agarose and gel filtration (Superdex 75)
-
nickel-affinity and gel filtration chromatography
phosphocellulose and Ni-NTA-agarose affinity chromatography
-
recombinant
-
recombinant enzyme
recombinant enzyme from Escherichia coli involving affinity chromatography
-
recombinant enzyme, fusion protein to a hexahistidyl tag
-
recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by two steps of nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged IDO1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged IDO2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged IDO1 from Escherichia coli strain BL21 Star by nickel affinity chromatography and gel filtration
F5H5G0, P14902
recombinant His6-tagged IDO2 from Escherichia coli strain BL21 Star by nickel affinity chromatography and gel filtration
F5H5G0, P14902
recombinant His6-tagged TDO from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to homogeneity
Superdex 200 gel filtration
-
the use of ALA, the biosynthetic precursor of protoporphyrin IX, coupled with metal-affinity chromatography and size exclusion chromatography produces 6His-IDP with a protein to heme ratio of 1:2.2
-
tissues thawed at room temperature, homogenized in 0.14 M potassium chloride plus 20 mM potassium phosphate, pH 7.0