1.13.11.9: 2,5-dihydroxypyridine 5,6-dioxygenase

This is an abbreviated version!
For detailed information about 2,5-dihydroxypyridine 5,6-dioxygenase, go to the full flat file.

Reaction

Synonyms

2,5-DHP dioxygenase, 2,5-dihydroxypyridine dioxygenase, 2,5-dihydroxypyridine oxygenase, 2,5DHP dioxygenase, EC 1.13.1.9, NicX, NicX protein, oxygenase, 2,5-dihydroxypyridine 5,6-di-, pyridine-2,5-diol dioxygenase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.9 2,5-dihydroxypyridine 5,6-dioxygenase

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Results	in table
3	Activating Compound
278	AA Sequence
2	Application
1	CAS Registry Number
4	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
3	General Information
7	General Stability
24	Inhibitors
2	KM Value [mM]
2	Localization
10	Metals/Ions
7	Molecular Weight [Da]
16	Natural Substrates/ Products (Substrates)
136	Organism
1	Oxidation Stability
7	Pathway
18	PDB ID
5	pH Optimum
2	Protein Variants
5	Purification (Commentary)
1	Reaction
3	Reaction Type
14	Reference
2	Source Tissue
5	Specific Activity [micromol/min/mg]
5	Storage Stability
28	Substrates and Products (Substrate)
8	Subunits
23	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Temperature Stability [°C]

Storage Stability

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Storage Stability on EC 1.13.11.9 - 2,5-dihydroxypyridine 5,6-dioxygenase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, stable for at least several weeks, enzyme in crude extract

Achromobacter sp.

-

396336

-40°C, 3 months, 20% loss of activity

Pseudomonas putida

-

396334

0°C, 20 mM sodium phosphate, pH 7.5, half-life: 2-3 days, addition of dithiothreitol extends half-life to about 2 weeks

Pseudomonas putida

-

396334

4°C, purified recombinant enzyme, 20 mM HEPES pH 7.5, 0.1 M NaCl, and 2 mM DTT, maintains its activity for several days

Pseudomonas putida

-

710759

4°C, several days, enzyme in crude extract

Achromobacter sp.

-

396336