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1.13.11.B6: linoleate 9/13-lipoxygenase

This is an abbreviated version!
For detailed information about linoleate 9/13-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.B6

Reaction

2 linoleate + 2 O2 =

(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
+
(9Z,11E)-13-hydroperoxy-9,11-octadecadienoate

Synonyms

13/9 lipoxygenase, 13/9-LOX, 9-/13-LOXN2, 9-lipoxygenase, 9-LOX, 9/13-lipoxygenase, 9/13-LOX, Ca-LOC-1, dual positional specific LOX, linoleate 9 S-lipoxygenase, linoleate oxygen oxidoreductase, linolenate 9R-dioxygenase, lipoxygenase, LOX, lox1-St-2, LOX1-Zm1, LOX1:Md:1a, LOX1a, LOX2:Md:2a, LOX2a, manganese 9S-lipoxygenase, MoLOX1, OsLOX1, PnLOX1, S-LOX

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.B6 linoleate 9/13-lipoxygenase

Cloned

Cloned on EC 1.13.11.B6 - linoleate 9/13-lipoxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein in Escherichia coli
expression in Escherichia col BL21(DE3)
expression in Escherichia coli
expression in Pichia pastoris
expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3)pLysS
gene LOX1a, RT-PCR and real-time quantitative PCR expression analysis, 22 putative LOX genes in apple, gene expression analysis throughout ripening reveals that only six LOXs are expressed in a ripening-dependent manner, overview. DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged or untagged LOX genes endoing for isozymes LOX1:Md:1a, L, X1:Md:1c, LOX2:Md:2a, and LOX2:Md:2b, containing an enterokinase cleavage recognition site in the first case, in Saccharomyces cerevisiae strain INVSc1, recombinant expression of YFP-tagged enzyme
gene LOX2a, RT-PCR and real-time quantitative PCR expression analysis, 22 putative LOX genes in apple, gene expression analysis throughout ripening reveals that only six LOXs are expressed in a ripening-dependent manner, overview. DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged or untagged LOX genes endoing for isozymes LOX1:Md:1a, L, X1:Md:1c, LOX2:Md:2a, and LOX2:Md:2b, containing an enterokinase cleavage recognition site in the first case, in Saccharomyces cerevisiae strain INVSc1, recombinant expression of YFP-tagged enzyme
recombinant expression in Pichia pastoris
-
sequence comparison and homology modelling
-