1.13.12.13: Oplophorus-luciferin 2-monooxygenase

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For detailed information about Oplophorus-luciferin 2-monooxygenase, go to the full flat file.

Reaction

Synonyms

19kOLase, CXXC-Oluc, imidazopyrazinone-type luciferase, KAZ, luciferase, nanoKAZ, NanoLuc, Oluc-19, Oplophorus luciferase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.13 Oplophorus-luciferin 2-monooxygenase

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Results	in table
1	AA Sequence
3	Application
8	Cloned(Commentary)
1	Crystallization (Commentary)
4	General Information
21	Inhibitors
18	Ki Value [mM]
4	KM Value [mM]
4	Localization
4	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
14	Organism
15	PDB ID
4	pH Optimum
29	Protein Variants
6	Purification (Commentary)
1	Reaction
14	Reference
1	Renatured (Commentary)
1	Source Tissue
4	Specific Activity [micromol/min/mg]
44	Substrates and Products (Substrate)
5	Subunits
18	Synonyms
1	Systematic Name
2	Temperature Stability [°C]

Cloned

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Cloned on EC 1.13.12.13 - Oplophorus-luciferin 2-monooxygenase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

construction of a cold induced expression vector in Escherichia coli cells that consists of a histidine tag sequence for nickel chelate affinity purification, IgG-binding domain of protein A (ZZ-domain) and the multiple cloning sites, the role of ZZ-domain as a solubilizing partner at 15°C is demonstrated by expressing the imidazopyrazinone-type luciferase of Oplophorus

Oplophorus gracilirostris

Q9GV45

696008

expressed in Escherichia coli

Oplophorus gracilirostris

-

727290

expression of 19 kDa subunit 19kOLase in Escherichia coli

Oplophorus gracilirostris

-

689868

recombinant expression and secretion of 19 kDa catalytic subunit mutant nanoKAZ from CHO-K1 cells. Mutant nanoKAZ lacks the amino-terminal signal peptide, is expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway. Recombinant expression of His-tagged wild-type and mutant KAZs in Escherichia coli. The secreted proteins form aggregates

Oplophorus gracilirostris

Q9GV45; Q9GV46

744223

recombinant expression and secretion, using the signal peptide sequence of Gaussia luciferase, of catalytic 19 kDa protein KAZ of Oplophorus luciferase and its mutants in Escherichia coli and recombinant expression of His-tagged wild-type and mutant enzymes and KAZs in CHO-K1 cells

Oplophorus gracilirostris

Q9GV45; Q9GV46

744220

recombinant expression of His-tagged 19 kDa catalytic subunit mutant nanoKAZ in Escherichia coli strain BL21. In bacterial cells, wild-type KAZ is mainly expressed as inclusion bodies

Oplophorus gracilirostris

Q9GV45; Q9GV46

744231

the catalytic domain of Oplophorus luciferase (19kOLase) is expressed in Escherichia coli cells using the cold-induced vectors of pCold-KAZ. Cold induction for Escherichia coli cells is carried out at 15°C

Oplophorus gracilirostris

-

728741

the fused proteinof nanoKAZ with IgG-binding domain (ZZ domain) is expressed in the cytoplasm of Escherichia coli cells as a soluble form and purified. A secretory expression of nanoKAZ in CHO-K1 cells is performed using nanoKAZ fused with the signal peptide sequence of Gaussia luciferase

Oplophorus gracilirostris

-

726882