1.13.12.13: Oplophorus-luciferin 2-monooxygenase

This is an abbreviated version!
For detailed information about Oplophorus-luciferin 2-monooxygenase, go to the full flat file.

Reaction

Synonyms

19kOLase, CXXC-Oluc, imidazopyrazinone-type luciferase, KAZ, luciferase, nanoKAZ, NanoLuc, Oluc-19, Oplophorus luciferase

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.12 With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

                1.13.12.13 Oplophorus-luciferin 2-monooxygenase

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Results	in table
1	AA Sequence
3	Application
8	Cloned(Commentary)
1	Crystallization (Commentary)
4	General Information
21	Inhibitors
18	Ki Value [mM]
4	KM Value [mM]
4	Localization
4	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
14	Organism
15	PDB ID
4	pH Optimum
29	Protein Variants
6	Purification (Commentary)
1	Reaction
14	Reference
1	Renatured (Commentary)
1	Source Tissue
4	Specific Activity [micromol/min/mg]
44	Substrates and Products (Substrate)
5	Subunits
18	Synonyms
1	Systematic Name
2	Temperature Stability [°C]

Substrates Products

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Substrates Products on EC 1.13.12.13 - Oplophorus-luciferin 2-monooxygenase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

3-hydroxy-2-methylimidazol[1,2-a]pyridine + O2

oxidized 3-hydroxy-2-methylimidazol[1,2-a]pyridine + CO2 + hn

Oplophorus gracilirostris

-

-

654469

-

-

?

3iso-coelenterazine + O2

oxidized 3iso-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 78.2%

728741

-

-

?

3me-coelenterazine + O2

oxidized 3me-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 80%

728741

-

-

?

3meo-coelenterazine + O2

oxidized 3meo-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 189%

728741

-

-

?

6h-coelenterazine + O2

? + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

-

744220

-

-

?

6h-coelenterazine + O2

oxidized 6h-coelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

luminescence intensity (Imax): 0.8

726882

-

-

?

6h-f-coelenterazine + O2

? + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

-

744220

-

-

?

6h-f-coelenterazine + O2

oxidized 6h-f-coelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

luminescence intensity (Imax): 10.1

726882

-

-

?

alphameh-coelenterazine + O2

oxidized alphameh-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 15.7%

728741

-

-

?

bis-coelenterazine + O2

? + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

-

744220

-

-

?

bis-coelenterazine + O2

oxidized bis-coelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

luminescence intensity (Imax): 10.3

726882

-

-

?

bisdeoxycoelenterazine + O2

oxidized bisdeoxycoelenterazine + CO2 + hn

Oplophorus gracilirostris

-

-

657385

-

-

?

bisdeoxycoelenterazine + O2

oxidized bisdeoxycoelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

native enzyme, 79%, catalytic subunit 19kOLase, 114% of the activity with coelenterazine, respectively

689868

-

-

?

cf3-coelenterazine + O2

oxidized cf3-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 49.5%

728741

-

-

?

coelenterazine + O2

coelenteramide + CO2 + hv

2 entries

coelenterazine + O2

oxidized coelenterazine + CO2 + hn

3 entries

coelenterazine + O2

oxidized coelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

-

689868

-

-

?

et-coelenterazine + O2

oxidized et-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 21.5%

728741

-

-

?

f-coelenterazine + O2

? + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

-

744220

-

-

?

f-coelenterazine + O2

oxidized f-coelenterazine + CO2 + hnu

2 entries

furimazine + O2

?

2 entries

furimazine + O2

? + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

-

744220

-

-

?

h-coelenterazine + O2

? + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

-

744220

-

-

?

h-coelenterazine + O2

oxidized h-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 68.4%

728741

-

-

?

h-coelenterazine + O2

oxidized h-coelenterazine + CO2 + hnu

2 entries

i-coelenterazine + O2

oxidized i-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 32.3%

728741

-

-

?

me-coelenterazine + O2

oxidized me-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 46.6%

728741

-

-

?

meo-coelenterazine + O2

oxidized meo-coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 68.1%

728741

-

-

?

Oplophorus luciferin + O2

oxidized Oplophorus luciferin + CO2 + hv

3 entries

additional information

?

-

2 entries

coelenterazine + O2

coelenteramide + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

-

744220, 744223, 744231

-

-

?

coelenterazine + O2

coelenteramide + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

a luciferin substrate

744231

-

-

?

coelenterazine + O2

oxidized coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

-

657385, 727290

-

-

?

coelenterazine + O2

oxidized coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intensity (Imax): 1.0

726882

-

-

?

coelenterazine + O2

oxidized coelenterazine + CO2 + hn

Oplophorus gracilirostris

-

luminescence intesity (Imax): 100%

728741

-

-

?

f-coelenterazine + O2

oxidized f-coelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

native enzyme, 26%, catalytic subunit 19kOLase, 80% of the activity with coelenterazine, respectively

689868

-

-

?

f-coelenterazine + O2

oxidized f-coelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

luminescence intensity (Imax): 19.5

726882

-

-

?

furimazine + O2

?

Oplophorus gracilirostris

-

-

727290

-

-

?

furimazine + O2

?

Oplophorus gracilirostris

-

luminescence intensity (Imax): 10.1

726882

-

-

?

h-coelenterazine + O2

oxidized h-coelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

native enzyme, 97%, catalytic subunit 19kOLase, 58% of the activity with coelenterazine, respcetively

689868

-

-

?

h-coelenterazine + O2

oxidized h-coelenterazine + CO2 + hnu

Oplophorus gracilirostris

-

luminescence intensity (Imax): 17

726882

-

-

?

Oplophorus luciferin + O2

oxidized Oplophorus luciferin + CO2 + hv

Diaphus gigas

-

-

674080

-

-

?

Oplophorus luciferin + O2

oxidized Oplophorus luciferin + CO2 + hv

Oplophorus gracilirostris

Q9GV45; Q9GV46

-

744220, 744223, 744231, 765340

-

-

?

Oplophorus luciferin + O2

oxidized Oplophorus luciferin + CO2 + hv

Oplophorus gracilirostris

-

-

765340

-

-

?

additional information

?

-

Oplophorus gracilirostris

-

luminescence intensity of the catalytic subunit 19kOLase alone is seven times lower for coelenterazine and three times lower for biscoelenterazine than that of native Oplophorus luciferase, respectively

689868

-

-

?

additional information

?

-

Oplophorus gracilirostris

Q9GV45; Q9GV46

Oplophorus luciferase shows broad substrate specificities for various coelenterazine analogues, and the substrate specificity is distinct from other coelenterazine-type luciferases including Renilla and Gaussia luciferases and the Ca2+-binding photoprotein aequorin

744220

-

-

?